Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 275-809-7 | CAS number: 71662-46-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994-02-01 to 1994-02-04
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): WITAMOL 118
- Substance type: product
- Physical state: liquid
- Stability under test conditions: no data
- Storage condition of test material: no data
- Other: none - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: WAF
- Sampling method: A sample for analysis was prepared identically to the test solution but without algal inoculation.
- Sample storage conditions before analysis: not mentioned - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1 g/L test substance was mixed with deionised water, stirred for 18 hours and subsequently filtered.
- Controls: yes, synthetic freshwater - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green algae
- Strain: Scenedesmus subspicatus (CHODAT (86.81 SAG))
- Source (laboratory, culture collection): Institut für Wasser-, Boden- und Lufthygiene, Berlin; subsequently in-house breeding
- Method of cultivation: in sterile-aerated Erlenmeyer flasks on light benches
ACCLIMATION
- Acclimation period: A preculture is withdrawn from a stock culture by tranfer 3 days prior to the beginning of the test from which preculture the test cultures are inoculated at a cell density of approximately 20 000 cells/mL.
- Culturing media and conditions (same as test or not): not mentioned
- Any deformed or abnormal cells observed: no data - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- none
- Hardness:
- Growth/test medium chemistry as described in guideline 92/69/EEC (C.3.), no further details mentioned
- Test temperature:
- 24 +/- 2 °C
- pH:
- 8.0 - 8.6 (start of test), 8.7 - 9.3 (end of test)
- Dissolved oxygen:
- not mentioned
- Salinity:
- not mentioned
- Nominal and measured concentrations:
- Nominal: WAF
Measured: 0.83 mg/L (geometric mean of values from the beginning and the end of the test) - Details on test conditions:
- TEST SYSTEM
- Test vessel: sterile-aerated Erlenmeyer flasks
- Initial cells density: 2x10E4 cells/mL
- Control end cells density: 88x10E4 cells/mL
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 8
- No. of vessels per vehicle control (replicates): not applicable
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
no details mentioned
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: not adjusted
- Photoperiod: not specified
- Light intensity and quality: approx. 8000 lux, white
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Cell numbers for exposure times of 24, 48, 72 h
- Determination of cell concentrations: The cell numbers were determined photometrically at a wavelenght of 685 nm
- Chlorophyll measurement: no
- Other: none
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not mentioned
- Range finding study: not applicable - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.83 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.83 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no observations made
- Any stimulation of growth found in any treatment: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no
Effective concentrations on the basis of cell growth:
EbC50 (72 h) > 0.83 mg/L
EbC10 (72 h) > 0.83 mg/L
EbC90 (72 h) > 0.83 mg/L
Effective concentrations based on the specific growth rate µ:
ErC50 (0-72 h) > 0.83 mg/L
ErC10 (0-72 h) > 0.83 mg/L
ErC90 (0-72 h) > 0.83 mg/L - Results with reference substance (positive control):
- no reference substance tested
- Reported statistics and error estimates:
- no statistical evaluation performed
- Validity criteria fulfilled:
- yes
- Conclusions:
- In the range of water solubility not toxic under test conditions.
- Executive summary:
The substance was n the range of water solubility not toxic under test conditions. The EC50 of 1,2-Benzenedicarboxylic acid, di-C8-10-alkyl esters was in excess of 0.81 mg/L.
Reference
Table #1: Cell density data:
concentration [mg/L] |
cell number (x 10E4 cells/ml) after | |||
0 h | 24 h | 48 h | 72 h | |
control | 2 | 13 | 28 | 88 |
0.83 | 2 | 10 | 37 | 114 |
Table #2: Growth curves:
concentration[mg/L] | biomass (area under the growth curve) | inhibition of biomass [%] | growth rate µ (0 - 72 h) | inhibition of growth rate [%] |
control | 80 | - | 1.261 | - |
0.83 | 99 | -23.8 | 1.348 | -6.9 |
The cloudiness of the test solution due to the product was considered
Description of key information
The substance was in the range of water solubility not toxic under test conditions. The EC50 of 1,2-Benzenedicarboxylic acid, di-C8-10-alkyl esters was in excess of 0.81 mg/L.
Key value for chemical safety assessment
Additional information
The substance was in the range of water solubility not toxic under test conditions. The EC50 of 1,2-Benzenedicarboxylic acid, di-C8-10-alkyl esters was in excess of 0.81 mg/L. Furthermore the toxicity of structurally related phthalates has been evaluated in several algae tests according to accepted guidelines with similar findings.
The ErC 50 for 1,2-Benzenedicarboxylic acid, di-C6-C10-alkyl esters in algae is above the limit of water solubility (ErC50 > 2.8 mg/l). These findings are supported by published data for this substance (Adams et al., 1995), resulting in EC50(96) > than the limit of water solubility. For 1,2-Benzenedicarboxylic acid, diundecyl ester there is only an acute effect level based on the cell number available resulting in EbC50 (96h) >2.1 mg/L. And the 96h-EC50 for 1,2-Benzenedicarboxylic acid, dihexyl ester was found to be greater than the highest concentration tested (>0.33 mg/l) and hence above the limit of water solubility.
The results are supported by results summarized in review articles for phthalates with an alkyl chain length of C6 and above.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.