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EC number: 500-457-0 | CAS number: 160901-19-9 1 - 2.5 moles ethoxylated
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 Nov 2020 - 29 Dec 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Version / remarks:
- adopted October 2012
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all concentrations
- Sampling method: Analytical samples taken at respective time points during the study course (exposure start, day 1 (aged), day 7 (fresh), day 8(aged), day 16 (fresh), day 17 (aged), day 20 (fresh), day 21 (aged))
- Sample storage conditions before analysis: The samples were mixed with 3 mL toluene and shaken by hand for 60 seconds. In the analytical laboratory, the toluene phase was separated (for aged samples sodium chloride was added for better phase separation, partly the extracts were centrifuged at 1800 rpm for 2 min.) and stored deep-frozen (≤ - 18 °C) until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution with a nominal concentration of 25 mg/L was prepared by adding the necessary amount of test item to a volumetric flask. Test medium was added and the solution was homogenised by shaking. The solution was observed to be clear and transparent and some foam was observed. Test item concentrations were prepared by dilution with test medium. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Clone V
- Age at study initiation (mean and range, SD): < 24 h
- Method of breeding: Daphnia magna was bred as single culture (1 daphnid per 100 mL) in Elendt M4 medium. pH-value: 6.0 – 9.0, dissolved oxygen: > 60 % saturation, total hardness 140 - 250 mg/L (as CaCO3), fed with single cell green algae (Desmodesmus subspicatus, formerly Scenedesmus subspicatus) at least three times a week.
- Source: bred at testing faciltiy, originally obtained from the Federal Environment Agency in Berlin/Germany.
- Feeding during test
- Food type: unicellular green algae (D. subspicatus) and additional nutrients (Tetra Min Baby®)
- Amount:
0.1 mg carbon / daphnia / day from day 0 to 7
0.15 mg carbon / daphnia / day from day 8 to 14
0.2 mg carbon / daphnia / day from day 15 to 20
- Frequency: daily
ACCLIMATION
- Acclimation period: none - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 214 - 250 mg/L CaCO3.
- Test temperature:
- 19.5 – 20.4 °C
- pH:
- 7.52 – 8.45
- Dissolved oxygen:
- 5.87 - 9.03 mg/L
- Nominal and measured concentrations:
- Nominal test substance concentrations: 0.00238, 0.00763, 0.0244, 0.0781, and 0.250 mg/L
Time weighted mean concentrations: 0.0019, 0.00437, 0.0123, 0.0401 and 0.131 mg/L (based on 3 representative constituents) - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers (nominal: 100 mL)
- Type (delete if not applicable): covered by glass plate
- Volume of solution: ≥ 50 mL
- Aeration: none
- Renewal rate of test solution (frequency): every 24 h
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Biomass loading rate: ≥ 50 mL / animal
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium
- Culture medium different from test medium: no
- Intervals of water quality measurement: Temperature, pH-value, oxygen concentration and total hardness was measured at test start and end and each weekday in all test item concentrations and control from fresh and aged solutions.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light and 8 hours darkness
- Light intensity: 1020 – 1498 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Dead animals were counted and removed daily. Observations (e.g. presence of eggs in the brood pouch, males or winter eggs, condition and size of parental generation) were recorded. Offsprings were counted and removed daily after appearance of first brood.
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.001, 0.0100, 0.100, and 1.00 mg/L
- Results used to determine the conditions for the definitive study: Mean number of alive offspring per alive adult at test end: 100 % mortality at 1 mg/L - Reference substance (positive control):
- no
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.012 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: based on three representative constituents
- Remarks:
- C12EO2, C12EO3, C12EO6
- Basis for effect:
- mortality: Number of living offspring produced per surviving parental animal {for Daphnia magna, TG 211}
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.043 mg/L
- 95% CI:
- >= 0.026 - <= 0.061
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: based on three representative constituents
- Remarks:
- C12EO2, C12EO3, C12EO6
- Basis for effect:
- mortality: Number of living offspring produced per surviving parental animal {for Daphnia magna, TG 211}
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.131 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: based on three representative constituents
- Remarks:
- C12EO2, C12EO3, C12EO6
- Basis for effect:
- mortality
- Remarks:
- of adult daphnia
- Details on results:
- - Other biological observations:
At nominal test item concentrations 2.38 to 78.1 µg/L no mortality above the allowed control mortality was observed. At the highest nominal test item concentration of 250 µg/L 30 % of the adult daphnids were dead at the end of exposure
- Mortality of control: no
- Other adverse effects control: no
- Immobilisation of control: no - Reported statistics and error estimates:
- The NOEC and LOEC were determined by using a multiple comparison method (Fisher’s exact test for mortality of adult daphnia, multiple Welch’s t-test with Bonferroni-Holm adjustment for alive offspring per adult from test start, Williams’ test for alive offspring per alive adult at test end). The EC20, 50- value for alive offspring per alive adult from test start and alive offspring per alive adult at test end was determined with 3 parameter logistic cumulative distribution function. The statistical program was not able to derive EC10-value of reproduction based on the data presented. Due to a weak dose response for EC20-values no reliable values were calculable and the EC20-values were not applicable. The LC50 for mortality of adult daphnia was not determined statistically.
- Validity criteria fulfilled:
- yes
- Remarks:
- Please see table 1 at "any other information on results incl. tables"
Reference
Table 1: Validity criteria for OECD 211
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The mortality of the parent animals in the control (female Daphnia) does not exceed 20% at the end of the test. |
0 % |
yes |
The mean number of living offspring produced per parent animalsurvivingat the end of the test is ≥ 60 in the control. |
69.3 |
yes |
Table 2: Sum of alive offspring at test end per adult from test start
Nominal conc. |
Mean number of alive offspring per adult |
Inhibition to control [%] |
|
|
|
Control |
69.3 |
- |
2.38 µg/L |
57.5 |
17.0 |
7.63 µg/L |
47.3 |
31.7 |
24.4 µg/L |
57.8 |
16.6 |
78.1 µg/L |
34.1* |
50.8 |
250 µg/L |
1.3* |
98.1 |
- not calculated
* statistically significant different from control
Table 3: Sum of alive offspring per surviving adult at the end of the test
Nominal conc. |
Mean number of alive offspring per alive adult at test end |
Inhibition to control [%] |
|
|
|
Control |
69.3 |
- |
2.38 µg/L |
61.6 |
11.1 |
7.63 µg/L |
47.3 |
31.7 |
24.4 µg/L |
57.8 |
16.6 |
78.1 µg/L |
33.0* |
52.4 |
250 µg/L |
1.9* |
97.3 |
- not calculated
* statistically significant different from control
Description of key information
NOEC (21 d): 0.0123 mg/L (TWA)
Key value for chemical safety assessment
Additional information
The chronic toxicity Alcohols, C12-13, branched and linear, ethoxylated to Daphnia magna was investigated in a GLP guideline study following OECD 211. The study was conducted under semi-static conditions with a daily renewal of test solutions. During the test period of 21 days the mortality of test organisms, time of first production of offspring and the number of offspring were assessed. The test organisms were exposed to nominal test item concentrations of 0.00238, 0.00763, 0.0244, 0.0781, and 0.250 mg/L. The test concentrations were analytically monitored by HPLC-MS/MS. The time weighted mean measured concentrations, based on three representative constituents of the UVCB substance, were 0.0019, 0.00437, 0.0123, 0.0401 and 0.131 mg/L. Based on the number of living offspring produced per surviving parental animal a NOEC (21 d) of 0.0123 mg/L (TWA) was observed.
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