Nickel sulphide

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study (OECD)

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

not specified

GLP compliance:

yes

Test type:

other:

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Nickel sulfide

EC Number:

234-349-7

EC Name:

Nickel sulfide

IUPAC Name:

Nickel sulfide

Details on test material:

- Name of test material (as cited in study report): Nickel sulfide
- Substance type: amorphous nickel sulfide, aqueos slurry
- Elemental composition: 52.4% Ni, 31.85% S

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River Breeding Laboratories, Inc., Wilmington, MA
- Age at study initiation: adult
- Weight at study initiation: ~ 292 g (males); ~199 (females)
- Fasting period before study: night before study
- Housing: Animals were individually housed in wire mesh bottom cages in environment controlled rooms.
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 5 days

Administration / exposure

Route of administration:

oral: unspecified

Vehicle:

not specified

Details on oral exposure:

MAXIMUM DOSE VOLUME APPLIED: 5000 mg/kg



CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose:
used a range finder

Doses:

Range Finding Study: 100, 266, 707, 1880, 5000 mg/kg
Mortality Study: 5000 mg/kg

No. of animals per sex per dose:

5 male; 5 females

Control animals:

no

Details on study design:

- Duration of observation period following administration: 15 days
- Frequency of observations and weighing: "frequently" on the day of exposure; twice daily thereafter
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Statistics:

Not reported.

Results and discussion

Preliminary study:

No mortality in rat groups (n=10) exposed to 5000 mg/kg at 15 days post-exposure.

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Mortality:

None.

Clinical signs:

other: Diarrhea (1/5 males; 0/5 females)

Gross pathology:

No noteworthy findings.

Other findings:

None.

Applicant's summary and conclusion

Conclusions:

Based on the data obtained from this study, the acute oral LD50 is considered to be greater than 5000 mg/kg bodyweight.