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EC number: 206-557-8 | CAS number: 354-33-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Basic toxicokinetics
Administrative data
- Endpoint:
- basic toxicokinetics in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: 2e Study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Pentahalotane-based chlorofluorocarbon substitutes and halotane: correlation of in vivo hepatic protein trifluoroacetylation and urinary trifluoro acetic acid excretion with calculated enthalpies of activation
- Author:
- Harris, J.W., Jones, J.P., Martin, J.L., La Rossa, A.C., Olson, M.J., Pohl, L.R. and Anders, M.W.
- Year:
- 1 992
- Bibliographic source:
- Chem. Res. Toxicol. 5, 720-25
Materials and methods
- Objective of study:
- metabolism
- GLP compliance:
- no
Test material
- Reference substance name:
- Pentafluoroethane
- EC Number:
- 206-557-8
- EC Name:
- Pentafluoroethane
- Cas Number:
- 354-33-6
- Molecular formula:
- C2HF5
- IUPAC Name:
- 1,1,1,2,2-pentafluoroethane
Constituent 1
- Radiolabelling:
- no
Test animals
- Species:
- rat
- Strain:
- Fischer 344
- Sex:
- male/female
Administration / exposure
- Route of administration:
- inhalation
- Vehicle:
- other: air
- Duration and frequency of treatment / exposure:
- 6 hour(s)
Doses / concentrations
- Remarks:
- Doses / Concentrations:
Males: 1%
- No. of animals per sex per dose / concentration:
- Males: 6
Results and discussion
Toxicokinetic / pharmacokinetic studies
Toxicokinetic parametersopen allclose all
- Toxicokinetic parameters:
- half-life 1st:
- Toxicokinetic parameters:
- half-life 2nd:
- Toxicokinetic parameters:
- half-life 3rd:
Metabolite characterisation studies
- Metabolites identified:
- yes
- Details on metabolites:
- Low metabolisation to trifluoroacetic acid (TFA) was observed
Any other information on results incl. tables
The
semi-quantitative analysis of immunoblotted TFA-proteins indicated a
decreasing potential to form TFA-proteins, in this order:
Halotane>=HCFC-123>>HCFC-124>HFC-125.
No quantitative analysis of TFA-protein amount was carried out.
TFA-proteins were not detected in samples from rats exposed to HFC-134a.
19F-NMR analysis of urinary TFA excretion after 12 hrs from the end of
exposure confirmed the previous data:
Halocarbon |
TFA excretion (umol/kg) |
halothane |
65 +/- 16 |
HCFC-123 |
82 +/- 20 |
HCFC-124 |
16 +-/ 2 |
HFC-125 |
1.7 +/- 1.7 |
Applicant's summary and conclusion
- Executive summary:
The study was aimed to determine the potential of various halogenated ethanes to form trifluoroacetylated-proteins following their metabolisation in the liver. Fisher rats were exposed to halothane (1.1%, n=6), HCFC-124 (1%, n=6), HFC-125 (0.97%, n=6), HCFC-123 (1.1%, n=6) and HFC-134a (1%, n=3) for 6 hours. At the end of the exposure, the urine were collected and analysed by 19F-NMR to measure the TFA excretion. 12 hours after after the exposure period termination, animals were sacrificed, liver was homogenized and cytosolic and microsomal fractions prepared. Protein of the subcellular fractions were separated by SDS-PAGE and immunoblotted with anti-TFA-protein serum.
HFC-125 showed a low potential to form TFA in liver, compared to other halogenated ethanes.
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