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EC number: 260-906-9 | CAS number: 57693-14-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Specific investigations: other studies
Administrative data
- Endpoint:
- cytotoxicity
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 10993, EN 7405: 1997
- Principles of method if other than guideline:
- The XTT test is based on the cleavage of the yellow tetrazolium salt XTT [= (sodium-3'-(1phenylaminocarbonyl)-3,4-tetrazolium)-bis-(4-metoxy-6-nitro)-benzenesulfonic acid hydrate)] to form an orange water soluble formazan dye by dehydrogenase activity in active mitochondria. This method was first described 1988 by SCUDIERO et al. (1,2) and improved in subsequent years by several other investigators.
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- in vitro
Test material
- Reference substance name:
- Trisodium bis[3-hydroxy-4-[(2-hydroxy-1-naphthyl)azo]-7-nitronaphthalene-1-sulphonato(3-)]chromate(3-)
- EC Number:
- 260-906-9
- EC Name:
- Trisodium bis[3-hydroxy-4-[(2-hydroxy-1-naphthyl)azo]-7-nitronaphthalene-1-sulphonato(3-)]chromate(3-)
- Cas Number:
- 57693-14-8
- Molecular formula:
- C40H20CrN6O14S2.3Na
- IUPAC Name:
- trisodium bis[3-hydroxy-4-[(2-hydroxy-1-naphthyl)azo]-7-nitronaphthalene-1-sulphonato(3-)]chromate(3-)
- Test material form:
- solid: particulate/powder
Constituent 1
Test animals
- Strain:
- other: ATCC, CCl 1 NCTC clone 929 (clone of strain l, mouse connective tissue) cellline
Administration / exposure
- Route of administration:
- other: in vitro culture
- Vehicle:
- not specified
- Duration of treatment / exposure:
- 24 hours
- Frequency of treatment:
- once
Doses / concentrationsopen allclose all
- Dose / conc.:
- 39.1 other: µg/ml
- Dose / conc.:
- 78.1 other: µg/ml
- Dose / conc.:
- 156.3 other: µg/ml
- Dose / conc.:
- 312.5 other: µg/ml
- Dose / conc.:
- 625 other: µg/ml
- Dose / conc.:
- 1 250 other: µg/ml
- Dose / conc.:
- 2 500 other: µg/ml
- Dose / conc.:
- 5 000 other: µg/ml
Examinations
- Examinations:
- Photometric determination of formazan dye from the cleavage of the yellow tetrazolium salt XTT [= (sodium-3'-(1phenylaminocarbonyl)-3,4-tetrazolium)-bis-(4-metoxy-6-nitro)-benzenesulfonic acid hydrate)]
- Positive control:
- 1. Solvent control for positive control: RPMI 1640 + 10 % (v/v) FCS + 10.0 % (v/v) deion. water
2. Solvent control for test item: RPMI + 10 % (v/v) FCS
3. Negative control: RPMI + 10 % (v/v) FCS
4. Positive control: SDS:
3.125 µg/ml; 6.25 µg/ml; 12.5 µg/ml; 25 µg/ml; 50 µg/ml; 100 µg/ml; 125 µg/ml; 250 µg/ml;
Results and discussion
- Details on results:
- Toxic effects were observed following incubation with test substance from 312.5 µg/ml up to the highest tested concentration (5000 µg/ml). The calculated XTT so value ís 464.4 µg/ml. Even after stringent washing not all of the test ítem could be removed from the cells in the higher concentrations leading to high chemical blank values and there with freak high viability values.
Applicant's summary and conclusion
- Conclusions:
- The test item possesses a cytotoxic potential.
- Executive summary:
Method
This in vitro study was performed to assess the cytotoxic potential of the test item by means of the XTT test using the mouse cell line L929. The following concentrations of the test item were tested: 39.1, 78.1, 156.3, 312.5, 625, 1250, 2500, 5000 µg/ml.
Complete medium (RPMI containing 10 % (v/v) FCS) was used as negative control.
The solvent control for the positive control was also RPMI medium containing 10 % (v/v) FCS and 10.0 % (v/v) deion. water. SDS was used as positive control.
The following concentrations were applied: 3.125, 6.25, 12.5, 25, 50, 100, 125, 250 µg/ml. The incubation time was 24 hours at 37 ± 1.5 °C.
Results
Negatíve control and solvent control showed no reduction in cell viability. The posítíve control (SDS) induced a distinct dose-related reduction in cell viabilíty.
Toxic effects were observed following incubation with the Test item from 312.5 µg/ml up to the highest tested concentration (5000 µg/ml). The calculated XTT50 value ís 464.4 µg/ml. Even after stringent washing not all of the test ítem could be removed from the cells in the higher concentrations leading to high chemical blank values and therewith freak high viability values. In conclusion, ít can be stated that in this study and under the experimental conditions reported, test item possesses a cytotoxic potential.
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