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EC number: 295-523-6 | CAS number: 92062-09-4 A complex combination of hydrocarbons obtained by treating slack wax with hydrogen in the presence of a catalyst. It consists predominantly of saturated straight and branched chain hydrocarbons having carbon numbers predominantly greater than C20.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 1985-11-05 to 1987-01-26
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study is classified as reliable without restriction because it was conducted according to or similar to guideline study OECD TG 473, without exceptions.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
- GLP compliance:
- no
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- 64741-52-2
- Cas Number:
- 64741-52-2
- IUPAC Name:
- 64741-52-2
- Reference substance name:
- light hydrotreated feedstock, insufficiently refined, IP 346 ≥ 3%
- IUPAC Name:
- light hydrotreated feedstock, insufficiently refined, IP 346 ≥ 3%
- Test material form:
- other: oily liquid
- Details on test material:
- Read across to unrefined and acid treated oils
- Name of test material (as cited in study report): L-06 Light Hydrotreated Feedstock
- Physical state: liquid
- Lot/batch No.: 96524
- Expiration date of the lot/batch: 09/19/90
- Stability under test conditions: not determined by Microbiological Associates, Inc.
- Storage condition of test material: room temperature
Constituent 1
Constituent 2
Method
- Target gene:
- ovary
Species / strain
- Species / strain / cell type:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 rat liver
- Test concentrations with justification for top dose:
- Dose levels of 0.2, 0.1, 0.05 and 0.02 uL/mL without S-9 activation and 0.03, 0.15, 0.08 and 0.03 uL/mL with S-9 activation were used.
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- yes
- Remarks:
- untreated cells
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- triethylenemelamine
- Remarks:
- Migrated to IUCLID6: Cyclophosphamide used for S-9 activated study
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Preincubation period: 16-24 hours
- Exposure duration: 10 non-activation, two hours for S-9 activated
- Expression time (cells in growth medium): 8 hours
- Fixation time (start of exposure up to fixation or harvest of cells): 10 hours
NUMBER OF CELLS EVALUATED: 100 metaphase cells were examined for each dose level.
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth - Evaluation criteria:
- The number of types of aberrations found was presented for each treatment group. The percentage of damaged cells in the total population of cells examined was calculated for each group. Frequency of structural aberrations per cell was calculated for each group.
- Statistics:
- Student's t test was used to analyze the frequency of structural aberrations per cell. Chi-square analysis using a 2x2 table was used to ascertain differences between the percentage of cells with numerical aberrations and each treatment group relative to the vehicle control.
Results and discussion
Test results
- Species / strain:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
-
RANGE-FINDING/SCREENING STUDIES: Dose levels for chromosome aberration assay were selected based on preliminary toxicity tests based on cell survival after treatment relative to the solvent control. CHO Cells were exposed to nine concentrations of test article ranging from 1.0 uL/mL to 0.0001 uL/mL in the presence and absence of an S-9 reaction mixture. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The positive and negative controls fulfilled the requirements for a valid test. Under the conditions of the assay, L-06 did not induce a statistically significant increase in the frequency of structural or numerical aberrations in the CHO cells, in the absence or presence of metabolic activation system. - Executive summary:
Read across justification
No in vitro chromosome aberration tests have been reported for slack waxes (carcinogenic or unknown feed-stock), but such studies have been reported for unrefined / acid treated lubricant base oils, materials similar to the oil entrained in slack waxes (carcinogenic or unknown feed-stock).
In a mammalian cell chromosome aberration study, CHO cell cultures were exposed to L-06 in DMSO at concentrations of 0.2, 0.1, 0.05 and 0.02 µL/mL without S-9 activation and 0.03, 0.15, 0.08 and 0.03 µL/mL with S-9 activation for 10 hours for non-activation and two hours for activation. L-06 was tested up to precipitating concentrations. Positive controls induced the appropriate response. The test material did not demonstrate mutagenciity in the chromosomal aberration assay using Chinese hamster ovary cells.
This study received a Klimisch score of 1 and is classified as reliable without restriction because it was conducted according to or similar to guideline study OECD TG 473.
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