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EC number: 915-673-4 | CAS number: 211519-85-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-09-03 to 2009-09-03
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: 46ul of the test substance which corresponds to 50 mg, was pipetted below the surface of the Milli-RO water and stirred briefly to mix (5 minutes). Synthetic sewage was subsequently feed and sludge added (final volume 500 ml) and the test was initiated. A loading rate of 100 mg/l was tested in duplicate.
- Sample storage conditions before analysis: Test substance stored at room temperature in the dark - Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Pretreatment: The sludge was coarsely sieved to remove large particles. The sludge was then washed by centrifuging at 1000 g for 10 minutes. The supernatent was decanted and replaced with ISO-medium. The washing step was performed three times. Small amount of the sludge was weighed and dried overnight at ca. 105 C to determine the amount of suspended solids.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2°C
- pH:
- 7.4
- Dissolved oxygen:
- 46 ul of test substance, Milli-RO water, synthetic sewage feed (16 ml) and activated sludge (200 ml) were mixed and made up to 500 ml with Milli-RO water in a 1 L bottle. The mixture was aerated during the contact time, using a pipette as an aeration device.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: All glass, approximately 300 ml oxygen bottles and 1 L test bottles
- Type (delete if not applicable): open
- Aeration: Strong aeration with clean, oil-free air
- Type of flow-through (e.g. peristaltic or proportional diluter): peristaltic
- No. of organisms per vessel: Number of micro-organisms was determined as the amount of mixed liquor suspended solids (MLSS) per litre of test medium.
- Biomass loading rate: 100 mg/l
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap-water was purified by reverse osmosis (Mill-RO) and subsequently passed over activated carbon and ion-exchange cartridges (Mill-Q).
- Reference substance (positive control):
- yes
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Reported statistics and error estimates:
- Regression line: Y = 43.61 X + 9.02
- Validity criteria fulfilled:
- yes
- Conclusions:
- An activated sludge respiratory inhibition test value EC50 exceeding a loading rate of 100 mg/L has been determined for Bis[3-(triethoxysilyl)propyl]polysulfides in a test conducted according to OECD 209. The result is considered reliable.
Reference
Table 2: Study plan
Flask |
Test Substance (µL) |
Synthetic sewage feed (mL) |
Stock sol. R 0.5 g/l (mL) |
Activated sludge (ml) |
Made up to 500 ml with Mill-RO water |
Exposure time (h) |
Conc/ loading rate (mg/L) |
C start R |
- |
16 |
- |
200 |
+ |
8.00 – 11.00 |
0 |
|
|||||||
R1 |
- |
16 |
5.0 |
200 |
+ |
8.15 – 11.15 |
5.0 |
R2 |
- |
16 |
12 |
200 |
+ |
8.30 – 11.30 |
12 |
R3 |
- |
16 |
30 |
200 |
+ |
8.45 – 11.45 |
30 |
|
|||||||
C end R |
- |
16 |
-- |
200 |
+ |
9.00 – 12.00 |
0 |
|
|||||||
C start T |
- |
16 |
- |
200 |
+ |
9.45 – 12.45 |
0 |
|
|||||||
T1 |
46 (=50 mg) |
16 |
- |
200 |
+ |
10.00 – 13.00 |
100 |
T2 |
46 (=50 mg) |
16 |
- |
200 |
+ |
10.15 – 13.15 |
100 |
|
|||||||
C end T |
- |
16 |
- |
200 |
+ |
10.30 – 13.30 |
0 |
C: Control
R: Reference substance, 3,5-dichlorophenol
T: Test substance, Bis[3-(triethoxysilyl)propyl]polysulfides
Table 3: Controls (C) and Bis[3-(triethoxysilyl)propyl]polysulfides (T): pH, oxygen concentration, oxygen consumption and percentage inhibition of the respiration rate
Flask |
loading rate (mg/l) |
Oxygen conc. At the start (~mg O2/l) |
Oxygen consumption (mg O2/l/h) |
% Respiration rate |
pH |
C start T |
0 |
7.4 |
29 |
- |
8.2 |
C end T |
0 |
7.9 |
28 |
- |
8.3 |
|
|||||
T1 |
100 |
5.3 |
29 |
-21 |
8.1 |
T2 |
100 |
7.1 |
30 |
-51 |
8.2 |
1: Negative values indicate stimulation of the respiration rate sludge. The values are considered not significant
Description of key information
ASRI 3-hour EC50: >100 mg/l (loading rate) (activated sludge respiration inhibition, OECD 209). Organisms were likely predominantly exposed to the parent test substance
Key value for chemical safety assessment
Additional information
An activated sludge respiration inhibition 3-hour EC50 value of >100 mg/l (loading rate) was determined for the substance in a reliable study conducted according to an appropriate test protocol, and in compliance with GLP. This indicates that the test substance was not toxic to waste water (activated sludge) bacteria at a loading rate of 100 mg/l. This study was chosen as the key study as it uses a more standard methodology than that used in the supporting study.
For the supporting study, a bacterial growth inhibition 16-hour EL10 value of >10 g/l (>10,000 mg/l) (nominal) was determined in a reliable study conducted according to an appropriate test protocol, and in compliance with GLP.
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