Dodecene, branched

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study (OECD 403 Inhalation Hazard Test). Deviation: 6 animals (3 males and 3 females) were used

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

yes

Remarks:

6 animals (3 males and 3 females) were used

GLP compliance:

no

Test type:

other: Inhalation Hazard Test

Test material

Test material information

Constituent 1

Reference substance name:

Dodecene, branched

EC Number:

306-479-5

EC Name:

Dodecene, branched

Cas Number:

97280-83-6

Molecular formula:

C12 H24

IUPAC Name:

(2Z)-4-methylundec-2-ene

Details on test material:

- Name of test material (as cited in study report): Isododecene
- Analytical purity: 99 %

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Dr. K. Thomae GmbH, 7950 Biberach, FRG
- Age at study initiation: about 8-9 weeks
- Weight at study initiation: mean weight of males was 283 g, mean weight of females was 192 g
- Housing: in groups of three in type D III wire mesh cages (without bedding)
- Diet (e.g. ad libitum): ad libitum in the period without exposure (KLIBA 24-343-4 rat/mouse laboratory diet)
- Water (e.g. ad libitum): ad libitum in the period without exposure
- Acclimation period: at least 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 24°C
- Humidity (%): 30 - 70%
- Photoperiod (hrs dark / hrs light): 12/12
There were no deviations from these set conditions which might have adversely affected the results of the study.

Animal identification: by dye marks at the tail

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

nose/head only

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: The product was introduced to a height of 5 cm into a glass bottle (generator) with a sintered glass disk (pore-size 90 - 150
pm, diameter 30 mm), and the weight was determined . The generator containing the product was placed in a waterbath maintained at 20°C by a the rmostat, and a stream of 200 l/h compressed air was supplied to a downstream mixing chamber .
- Method of holding animals in test chamber: The mixture of air and test substance was passed through a glass distributor to 6 glass tubes in which 3 male and 3 female animals had been placed .
- Source and rate of air: 200 1/h compressed air

Suitable measures were applied to ensure that the air supply had the same temperature as in the laboratories, so that the temperature
in the exposure apparatus was between 19 and 25°C .
There were no deviations from these set conditions which might have adversely affected the results of the study .
After 30 minutes, the generator was replaced by a new one containing fresh test substance as described above . This generator was then used for the remainder of the test .

Analytical verification of test atmosphere concentrations:

yes

Remarks:

The amount of test substance used was determined by reweighing the generators . The nominal concentration was calculated from the amount of test substance consumed and the air volume .

Duration of exposure:

7 h

Concentrations:

3 .8 mg/l (calculated) .

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: Clinical examinations took place each workday . Lethality was checked each day
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 3.8 mg/L air (nominal)

Exp. duration:

7 h

Remarks on result:

other: vapour

Mortality:

All animals survived.

Clinical signs:

other: During exposure: Clinical signs and findings which were observed during exposure are presented in the following table. After exposure and during observation period: No abnormalities were detected in the animals

Gross pathology:

No pathologic findings were noted.

Any other information on results incl. tables

Time after beginning of exposure	1 min	15 min	30 min	1 h	2 h	3 h	4 h	5 h	6 h	7 h
accelarated respiration	6	6	6	6	6	6	6	6	6	6
eyelid closure	6
attempts to escapte	6

The numbers indicate, how many female and male animals showed these clinical signs and findings

.