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EC number: 203-299-8 | CAS number: 105-45-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- December 2008 until January 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: study performed in accordance with the corresponding OECD-/EU-testing guidelines
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Methyl acetoacetate
- EC Number:
- 203-299-8
- EC Name:
- Methyl acetoacetate
- Cas Number:
- 105-45-3
- Molecular formula:
- C5H8O3
- IUPAC Name:
- methyl 3-oxobutanoate
- Test material form:
- other: non-viscous clear liquid
- Details on test material:
- - Physical state: Colourless liquid
- Storage condition of test material: Room temperature, protected from light
- Stability in solvent: > 72 h in DMSO and ethanol at room temperature
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: Harlan LAboratories BV, 5960 AD Horst, The NEtherlands
- Age at study initiation: 8-12 weeks
- Weight at study initiation: 21.8-23.0 grams priotr treatment with 3HTdR
- Housing: single housing
- Diet (e.g. ad libitum): Pelleted standard diet, Harlan Lab. GmbH, 33178 Borchen, Germany
- Water (e.g. ad libitum): Tap water
- Acclimation period: At least 5 days prior to the start of dosing
ENVIRONMENTAL CONDITIONS
- Temperature: 22°C (+/- 3°C)
- Humidity: 30-70%
- Photoperiod: artificial light 06:00 a.m. - 06:00 p.m.
Study design: in vivo (LLNA)
- Vehicle:
- dimethyl sulphoxide
- Concentration:
- Doses of 25, 50 and 100& (in DMSO) were applied.
- No. of animals per dose:
- 4 animals per dose group
- Details on study design:
- Administration and exposure:
For determination of the highest non-irritant and technically applicable test item concentration, a pretest was performed on two mice with concentrations of 50 and 100 % (w/v). The top dose of the test item is the highest technically achievable concentration whilst avoiding systemic toxicity and excessive local irritation. Four female mice were treated with different concentrations of the test item and vehicle alone by topical application at the dorsum of each ear lobe (left and right) on three consecutive days. Five days after the first topical application, the mice were intravenously injected into a tail vein with radiolabelled thymidine (3H-methyl thymidine). Five hours after intravenous injection, the mice were sacrificed and the draining auricular lymph nodes excised and pooled per group. Single cell suspensions of lymph node cells were prepared from pooled lymph nodes which were subsequently washed and incubated with trichloroacetic acid overnight. The proliferative capacity of pooled lymph node cells was determined by the incorporation of 3H-methylthymidine measured in a ß-scintillation counter.
Data and Observations:
The proliferative response of lymph node cells is expressed as the number of radioactive disintegrations per minute per lymph node and as the ratio of 3HTdR incorporated into lymph node cells of test lymph nodes relative to that recorded for control lymph nodes (stimulation index). In addition to the sensitising reactions the following observations and data were recorded: mortality/viability once daily, body weights prior to the first application and prior to necropsy, clinical signs (local/systemic) daily from start to the termination of in-life phase. - Positive control substance(s):
- hexyl cinnamic aldehyde (CAS No 101-86-0)
- Statistics:
- The mean values amd statndard deviations were calculated in the body and ear weight tables.
Results and discussion
- Positive control results:
- Results of the GLP Positive Control:
- Experiment performed in July 2008.
- Positive control substance: Hexylcinnamaldehyde
- Vehicle: acetone: olive oil (4+1)
- Results: at dose concentrations of 5, 10 and 25% (w/v), stimulation indices of 5.24, 7.38 and 9.32 were observed
The EC3 value was estimated to be < 5%. A precise calculation of the EC3 value was not possible since the S.I. was above the threshold of 3 even at the lowest tested concentration. The obtained values are within the expected range and the positive control experiment is considered fully valid.
In vivo (LLNA)
Resultsopen allclose all
- Key result
- Parameter:
- SI
- Value:
- 1.53
- Test group / Remarks:
- Test concentration 25%
- Remarks on result:
- other: A calculation of the EC3 value was not possible since none of the tested concentrations induced a S.I. greater than 3.
- Key result
- Parameter:
- SI
- Value:
- 1.91
- Test group / Remarks:
- Test concentration 50%
- Remarks on result:
- other: A calculation of the EC3 value was not possible since none of the tested concentrations induced a S.I. greater than 3.
- Key result
- Parameter:
- SI
- Value:
- 0.7
- Test group / Remarks:
- Test concentration 100%
- Remarks on result:
- other: A calculation of the EC3 value was not possible since none of the tested concentrations induced a S.I. greater than 3.
Any other information on results incl. tables
Test item concentration % (w/v) |
Group |
Measurement DPM |
Calculation |
Result |
||
DPM-BG a)
|
number of lymph nodes |
DPM per lymph node b) |
S.I.
|
|||
--- |
BG I |
33 |
--- |
--- |
--- |
--- |
--- |
BG II |
37 |
--- |
--- |
--- |
--- |
--- |
1 |
4571 |
4536 |
8 |
567.0 |
|
25 |
2 |
6966 |
6931 |
8 |
866.4 |
1.53 |
50 |
3 |
8719 |
8684 |
8 |
1085.5 |
1.91 |
100 |
4 |
3198 |
3163 |
8 |
395.4 |
0.70 |
BG = Background (1 ml 5% trichloroacetic acid) in duplicate
1 = Control Group
2-4 = Test Group
S.I. = Stimulation Index
a) = The mean value was taken from the figures BG I and BG II
b) = Since the lymph nodes of the animals of a dose group were pooled, DPM/node was determined by dividing the measured value by the number
of lymph nodes pooled
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The test item Methyl acetoacetate was not a skin sensitiser under the described conditions.
- Executive summary:
The LLNA-study was performed in 2008/2009 under GLP. In order to study a possible contact allergenic potential of Methyl acetoacetate, three groups each of four female mice were treated daily with the test item at concentrations of 25, 50, and 100 % (w/v) in dimethylsulfoxide by topical application to the dorsum of each ear lobe (left and right) for three consecutive days. A control group of four mice was treated with the vehicle (dimethylsulfoxide) only. Five days after the first topical application the mice were injected intravenously into a tail vein with radio-labelled thymidine (3H-methyl thymidine). Approximately five hours after intravenous injection, the mice were sacrificed, the draining auricular lymph nodes excised and pooled per group. Single cell suspensions of lymph node cells were prepared from pooled lymph nodes, which were subsequently washed and incubated with trichloroacetic acid overnight. The proliferative capacity of pooled lymph node cells was determined by the incorporation of 3H-methyl thymidine measured in a beta-scintillation counter.
All treated animals survived the scheduled study period and no signs of toxicity were observed. An increase in the ear weights of the treated animals was also not observed. In this study Stimulation Indices of 1.53, 1.91, and 0.70 were determined with the test item at concentrations of 25, 50, and 100 % in dimethylsulfoxide. The EC3 value could not be calculated, since none of the tested concentrations induced an S.I. greater than 3.
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