Chromium trinitrate

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: summarized in this publication without detailed information, contributing to assessment

Data source

Reference

Reference Type:

publication

Title:

Differential toxicity and clearance kinetics of chromium(III) or (VI) in mice.

Author:

Bryson WG, Goodall CM (1983)

Year:

1983

Bibliographic source:

Carcinogenesis, 4:1535–1539.

Report date:

1983

Materials and methods

Test guideline

Qualifier:

no guideline available

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

chromium nitrate

IUPAC Name:

chromium nitrate

Test material form:

not specified

Details on test material:

These solutions were sufficiently concentrated to maintain pH below 2.5 by hydrolysis and thus prevent the olation and oxolation reactions and subsequent polymerisation that can otherwise occur.

Test animals

Species:

mouse

Strain:

other: NZC

Sex:

male

Details on test animals or test system and environmental conditions:

Mice were (CxO) Fl hybrids ( 9 NZC/BlGd x a NZO/BlGd) of both sexes and NZC males from the original Cancer Research Department colonies (21).
After weaning at age 4 weeks they were kept in polycarbonate cages29 x 18 x 13 cm) with untreated wood shavings as litter in a room thermostatted at 22 °C and supplied with positive pressure, humidified and filtered entilation.

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

not specified

Details on exposure:

exposed mice to 6 dosage levels of Cr(NO3)3.9H2O, calculated LD50 10 days after interperitoneal injection
Male and female mice aged 5—6 months and averaging 40 g body weight were randomised into groups of 3, and group treatments then fixed by a further randomisation using Fisher and Yates tables (26). Treatments involved i.p. injections of equimolar solutions of chromium(III) (3.25 /µg/g mouse) given either once or at weekly intervals. Dosages corresponded to one-sixth of the distal LD50 per injection.
Mice treated once were killed at 3, 7 or 21 days after treatment, and those injected repeatedly were killed at 1 —4, 8, 10, 12 and 14 weeks. After 8 weeks
the injections were every 2 weeks instead of weekly.

Doses:

6 dose levels, detailed information is not available

No. of animals per sex per dose:

4

Control animals:

not specified

Details on study design:

Mice were weighed and examined weekly. Treatments began at age 5-6 months and at various times mice were killed under ether anaesthesia and the carcasses stored sealed in plastic bags.

Statistics:

utilising at least 5 dose levels and 4 mice per level, Statistical comparisons were by Student's t-test.

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

39.1 mg/kg bw

Based on:

element

Remarks:

chromium(III)

95% CL:

> 8.6 - < 36.9

Remarks on result:

other: for Cr(NO3)3 no LD50 was determined but an average of 39.1 mg/kg was reported as average chromium(III) sulphate and chromium trichloride.

Effect levels 2

Sex:

male/female

Dose descriptor:

other: distal median lethal dose

Effect level:

17.8 mg/kg bw

Based on:

element

Remarks:

chromium(III)

95% CL:

>= 8.6 - <= 36.9

Remarks on result:

other: for chromium trinitrate

Mortality:

No death

Clinical signs:

N/A

Body weight:

N/A

Gross pathology:

N/A

Applicant's summary and conclusion

Conclusions:

LD50 of chromium trinitrate for acute toxicity is concluded to be 17.8 mg chromium (III)/kg bw.

Executive summary:

The acute toxicities of the chromium(III) nitrate after i.p. injection were determined in NZC male mice according to Weil's method. The distal LD5o 10 days after injection is calculated to be 17.8 (8.6 -36.9) mg chromium (III)/kg body weight, which were summarized in this report without detailed information.