3-(methylthio)propionaldehyde

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Remarks:

Duration of exposure only 24 h.

Qualifier:

according to guideline

Guideline:

ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 1: Static Method)

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was directly added the te test medium. The solution was homogenised with an ultra-turrax.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

.TEST ORGANISM
- Common name: zebrafish
- Source: Centre de Recherche de la Station de Lagunage de Meze, Meze, France
- Length at study initiation (length definition, mean, range and SD): 3.5 ± 0.3 cm
- Weight at study initiation (mean and range, SD): 0.44 ± 0.21 g

ACCLIMATION
- Acclimation period: 14 d
- Acclimation conditions (same as test or not): dilution water at 23 °C ± 1°C
- Type and amount of food during acclimation: not given
- Feeding frequency during acclimation: daily except 24 h before the test
- Health during acclimation (any mortality observed): No desease was observed.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Test temperature:

21.9 - 22.8 °C

pH:

7.44 - 7.84

Dissolved oxygen:

93 - 96 % at 0 h
82 - 86 at 24 h

Nominal and measured concentrations:

Nominal test substance concentrations: 0, 5.5, 9.1, 12.5, 18.1, 27.7, 40.3, 57.7 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria 28x28x28cm
- Fill volume: 10 L
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionised water, reconstitued with
CaCl2 x 2H2O = 0.294 g/L
MgSO4 x 7H2O = 0.12325 g/L
NaHCO3 = 0.06475 g/L
KCl =0.00575 g/L
- Intervals of water quality measurement: pH and qxygen at 0 and 24 h

OTHER TEST CONDITIONS
- Light intensity: 1000 to 2000 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable)
- Mortality after 24 h

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 1.1, 3.7, 10.1, 42.2, 106, 372, 1076 mg/L
- Results used to determine the conditions for the definitive study: LC50 (24 h): 21 mg/L

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

24 h

Dose descriptor:

LC0

Effect conc.:

9.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

14 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

24 h

Dose descriptor:

LC100

Effect conc.:

18.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: starting at 4 h and a concentration of 40.3 mg/L slower swimming was observed. At 4h and a concentration of 57.7 mg/L swimming at the bottom of the test vessel and immobilised test fish were observed.
- Observations on body length and weight: lenght 3.5 ± 0.3 cm, weight: 0.44 ± 0.21 g

Results with reference substance (positive control):

LC50 (24 h): 210 mg/L

Reported statistics and error estimates:

log-probit analysis

Sublethal observations / clinical signs:

Table with the cumulative mortality:

concentration (mg/L)	Mortality (number of fish)	Mortality (%)
0	0	0
5.5	0	0
9.1	0	0
12.5	1	10
18.1	10	100
27.7	10	100
40.3	10	100
57.7	10	100

 

Validity criteria fulfilled:

no

Conclusions:

In a test conducted with Danio rerio the determined LC50 after 24 h was 14 mg/L.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992

GLP compliance:

not specified

Analytical monitoring:

yes

Details on sampling:

- Sampling method: At the beginning of exposure, before water change

Vehicle:

not specified

Test organisms (species):

Oryzias latipes

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

24 ± 1°C

Nominal and measured concentrations:

Nominal test substance concentrations: 3.95, 5.93, 8.89, 13.3 and 20.0 mg/L

Details on test conditions:

TEST SYSTEM
- Fill volume: 25 L
- Renewal rate of test solution (frequency): every 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light / 8 hours dark
- Light intensity: Indoor light

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.5

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

6.95 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% confidence limit: 5.95 to 8.33 mg/L

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

3.19 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

12.3 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Description of key information

LC50 (96 h): 6.95 mg/L (meas., OECD 203)

Additional information

Two studies on the acute toxicity of the substance to freshwater fish are available. One study was conducted according to OECD 203 with Oryzas latipes in a semi-static test system. The nominal test substance concentrations of 3.95, 5.93, 8.89, 13.3 and 20.0 mg/L were re-newed every 24 hours. An analytical dose verification by HPLC analysis was performed. The determined LC50 (96 h) was 6.95 mg/L (measured). A second available test was performed according to ISO guideline 7346-1 (GLP). Zebrafish (Danio rerio) were exposed to nominal test substance concentrations of 0, 5.5, 9.1, 12.5, 18.1, 27.7, 40.3, 57.7 mg/L for 24 h. No analytical verification of the test item concentrations was performed. The mortality of fish was measured after 24 h resulting in a LC50 of 14 mg/L based on nominal concentrations.

However, since the test duration was only 24 h the study is not sufficient and this result is not used for the assessment.