Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-269-1 | CAS number: 93-70-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990-03-05 to 1990-03-25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study documented and performed according to GLP standards and in compliance with OECD Guideline 471.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable.
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2'-chloroacetoacetanilide
- EC Number:
- 202-269-1
- EC Name:
- 2'-chloroacetoacetanilide
- Cas Number:
- 93-70-9
- Molecular formula:
- C10H10ClNO2
- IUPAC Name:
- 2'-chloroacetoacetanilide
- Details on test material:
- Identity: P0005
Storage: RT
Expiry date: August 13, 1992
Appearance: White powder
Constituent 1
Method
- Target gene:
- Not applicable.
Species / strain
- Species / strain / cell type:
- other: TA 1535, TA 1537, TA 1538, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- TA 1535 his G46 rfa uvrB
TA 1537 his C3076 rfa uvrB
TA 1538 his D3052 rfa uvrB
TA 98 his D3052 rfa uvrB pKM 101
TA 100 his G46 rfa uvrB pKM 101 - Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- Mixed-function oxidase systems in the rat liver were stimulated following a single i/p injection of Aroclor 1254
- Test concentrations with justification for top dose:
- Dose range finding test: 5000, 500, 50, 5 microg/plate.
Mutation tests: 5000, 1500, 500, 150, 50 microg/plate. - Vehicle / solvent:
- Dimethylsulphoxide
Controls
- Untreated negative controls:
- yes
- Remarks:
- dimethylsulphoxide
- Negative solvent / vehicle controls:
- yes
- Remarks:
- dimethylsulphoxide
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene, 9-Aminoacridine, N-Ethyl-N'-nitro-N-nitrosoguanidine, 2-Nitrofluorene
- Details on test system and experimental conditions:
- For use in tests sub-cultures were grown in Nutrient Broth No. 2 (Oxoid) at 37°C for 10 hours. This culture provided approximately 2 x 10x9
organisms per ml which was assessed photometrically.
Preparation of liver homogenate S—9 fraction:
Species: Rat.
Strain: Sprague—Dawley derived.
Source: Bantin and Kingman Ltd., The Field
Station, Grimston, Aldbrough, Hull,
North Humberside, HU11 4QE.
Age range: 7 — 8 weeks.
Weight range: <300 g.
Diet: Labsure Rodent Diet LAD 1.
Stimulation of rat liver enzymes:
Mixed—function oxidase systems in the rat liver were stimulated following a single i/p injection of Aroclor 1254 (diluted in Arachis oil to 200 mg/ml) at a dosage of 500 mg/kg. On the fifth day of induction, following an overnight starvation, the rats were killed and their livers aseptically removed. - Evaluation criteria:
- The mean number of revertant colonies for all treatment groups is compared with those obtained for solvent control groups. The mutagenic activity
of a test material is assessed by applying different criteria.
Revertant colonies were counted using a Biotran Automatic Colony Counter. Any toxic effects of the test substance can be detected by a substantial
reduction in revertant colony counts or by the absence of a complete background bacterial lawn. - Statistics:
- Not applicable.
Results and discussion
Test results
- Species / strain:
- other: TA 1535, TA 1537, TA 1538, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- In the preliminary toxicity test P0005 was not toxic towards the tester strains.
Therefore, 5000 microg/plate was chosen as the top dose level in the mutation tests.
The mean number of revertant colonies, together with the individual plate counts for P0005 obtained in the first and second mutation test with the
tester strains are shown in the Table below.
No substantial increases in revertant colony numbers of any of the tester strains were observed following treatment with P0005 at any dose level,
either in the presence or absence of S-9 mix. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Strain |
Dose level (microg/plate) |
Metabolic activation -/+ |
Mean revertant colony counts |
TA 1535 |
5000 1500 500 150 50 0 Solvent |
- / + - / + - / + - / + - / + - / + - / + |
7 / 9 7 / 12 8 / 11 9 / 10 10 / 9 8 / 9 7 / 11 |
TA 1537 |
5000 1500 500 150 50 0 Solvent |
- / + - / + - / + - / + - / + - / + - / + |
9 / 7 7 / 7 12 / 10 6 / 6 7 / 9 5 / 7 7 / 6 |
TA 1538 |
5000 1500 500 150 50 0 Solvent |
- / + - / + - / + - / + - / + - / + - / + |
7 / 7 7 / 7 7 / 11 5 / 7 8 / 9 7 / 9 11 / 13 |
TA 98 |
5000 1500 500 150 50 0 Solvent |
- / + - / + - / + - / + - / + - / + - / + |
16 / 11 18 / 18 21 / 17 18 / 19 20 / 17 20 / 17 19 / 18 |
TA 100 |
5000 1500 500 150 50 0 Solvent |
- / + - / + - / + - / + - / + - / + - / + |
61 / 55 68 / 78 69 / 78 81 / 88 69 / 83 72 / 95 81 / 97 |
- Absence
+ Presence
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative without metabolic activation
negative with metabolic activation
lt is concluded that, when tested at dose levels up to 5000 microg/plate in dimethylsulphoxide, P0005 was not mutagenic in this bacterial test system. - Executive summary:
The genetic toxicity study in vivo (Ames) was performed in year 1990 according to GLP standards and in compliance with OECD Guideline 471.
In the preliminary dose range finding study with dose levels of up to 5000 microg/plate no toxicity was observed. A top dose level of 5000 g/plate was chosen for the subsequent mutation study. Other dose levels used in the mutation assays were: 1500, 500, 150, 50 microg/plate. The concurrent positive control compounds demonstrated the sensitivity of the assay and the metabolising activity of the liver preparations. No evidence of mutagenic activity was seen at any dose level of P0005 in either mutation test.
Therefore, it is concluded that the test item is not mutagenic in this bacterial system.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.