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EC number: 210-871-0 | CAS number: 624-92-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: other routes
Administrative data
- Endpoint:
- acute toxicity: other routes
- Type of information:
- experimental study
- Study period:
- 22 March 2005 to 13 April 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- GLP compliance:
- yes
- Limit test:
- no
Test material
- Reference substance name:
- Dimethyl disulphide
- EC Number:
- 210-871-0
- EC Name:
- Dimethyl disulphide
- Cas Number:
- 624-92-0
- Molecular formula:
- C2H6S2
- IUPAC Name:
- (methyldisulfanyl)methane
- Details on test material:
- Test article : Dimethyl disulphide
Batch number: 06025PB (TM 16-1-2)
Purity (%): 99.8
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River (UK) Ltd, Margate
- Age at study initiation: approximately 7 to 9 weeks old on Day 1
- Weight at study initiation: 217 to 254 g (males) and 185 to 227 g (females) on Day -1
- Fasting period before study: no data
- Housing: up to five during acclimatisation and in pairs during the study
- Diet (ad libitum): SQC Rat and Mouse Maintenance Diet No 1, Expanded
- Water (ad libitum): Mains water
- Acclimation period: 7 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19 to 25
- Humidity (%): 40 to 70
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- intravenous
- Vehicle:
- other: Intralipid 20, Fresenius, Basingstoke, UK.
- Details on exposure:
- Each rat was dosed once on Day 1, by single intravenous bolus injection into a tail vein. The rat was held in a restraint device throughout the dosing procedure. Plastic syringes and disposable hypodermic needles were used.
Individual dose volumes (mL) were calculated using the body weights of the rats on the morning of dosing (Day 1) and the selected dose volume (2 mL/kg). - Doses:
- 1, 5 and 50 mg/kg bw
- No. of animals per sex per dose:
- 2
- Control animals:
- no
- Details on study design:
- EXPERIMENTAL OBSERVATIONS
- Clinical signs
Treated rats were observed closely for clinical signs of reaction to treatment. Clinical signs were recorded immediately post-dose (50 mg/kg dose group only), at approximately 15 and 30 minutes post-dose, hourly between 1 and 4 hours post-dose (inclusive), twice daily on Days 2, 3 and 4 and once daily from the fifth to last day of the observation period. Individual records of clinical signs and times of death were maintained for each treated rat.
- Body weights
Rats were weighed on Day –1, on Day 1, Day 4 and Day 8. The weight of the carcass of each animal found dead or killed before Day 8 was also recorded.
TERMINAL PROCEDURES
- Necropsy
Animals dying during the observation period were subject to necropsy with the least possible delay. Carcasses may be stored in a refrigerator until necropsy commences. The necropsy procedure included inspection of external surfaces and orifices, all viscera and tissue within the abdominal, thoracic and cranial cavities, free-hand sectioning of the liver and kidneys and examination of representative sections of mucosal surfaces of the stomach, small and large intestines.
Rats surviving treatment were killed by intraperitoneal injection of sodium pentobarbitone on Day 8. No necropsies were performed on these animals.
Results and discussion
- Mortality:
- One male dosed at 50 mg/kg died approximately 5 minutes after dosing. The second male dosed at 50 mg/kg died approximately 10 minutes after dosing. No other mortalities were noted.
- Clinical signs:
- Clinical signs of reaction to treatment noted in animals dosed at 50 mg/kg were prone posture, limpness, cyanosis, dyspnoea, lethargy and hunched posture. Recovery of the females, as judged by external appearance and behaviour, was complete by one hour after dosing.
No clinical signs were noted in animals dosed at 1 mg/kg or 5 mg/kg except for an isolated incident of bloated abdomen noted in on Day 3 in one male dosed at 1 mg/kg. - Body weight:
- All surviving rats achieved body weight gains during the study period.
- Gross pathology:
- Macroscopic changes noted at necropsy of animals that died during the study were mottled and dark liver and enlarged atria of the heart.
Applicant's summary and conclusion
- Conclusions:
- Dimethyl disulphide, was found to be well-tolerated in female rats at the dose levels administered, but was not tolerated in male rats at 50 mg/kg.
- Executive summary:
Three groups of two male and two female rats were given the test article as a single dose on Day 1 by intravenous injection at dose levels of 1, 5 and 50 mg/kg. Dimethyl disulphide was dispersed in vehicle ( Intralipid 20) and administered at a dose volume of 2 mL/kg. Animals surviving treatment were killed on Day 8. Necropsies were only performed on animals that died during the observation period.
Both males dosed at 50 mg/kg died within 10 minutes of dosing. No other mortalities were noted. Clinical signs of reaction to treatment noted in animals dosed at 50 mg/kg were prone posture, limpness, cyanosis, dyspnoea, lethargy and hunched posture. Recovery of the females, as judged by external appearance and behaviour, was complete by one hour after dosing. No clinical signs were noted in animals dosed at 1 mg/kg or 5 mg/kg except for an isolated incident of bloated abdomen noted in on Day 3 in one male dosed at 1 mg/kg. All surviving rats achieved body weight gains during the study period. Macroscopic changes noted at necropsy of animals that died during the study were mottled and dark liver and enlarged atria of the heart. Dimethyl disulphide, was found to be well-tolerated in female rats at the dose levels administered, but was not tolerated in male rats at 50 mg/kg.
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