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Diss Factsheets
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EC number: 201-758-7 | CAS number: 87-62-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian germ cell study: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Basic data given, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Chemical Mutagenesis Testing in Drosophila . X. Results of 70 Coded Chemicals Tested for the National Toxicology Program
- Author:
- Foureman P, et al.
- Year:
- 1 994
- Bibliographic source:
- Environ. Mol. Mutagen. 23, 208-227
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 477 (Genetic Toxicology: Sex-linked Recessive Lethal Test in Drosophila melanogaster)
- Principles of method if other than guideline:
- Seventy chemicals were tested for the ability to induce sex-linked recessive lethal (SLRL) mutations in postmeiotic and meiotic germ cells of male Drosophila melanogaster.
- GLP compliance:
- not specified
- Type of assay:
- Drosophila SLRL assay
Test material
- Reference substance name:
- 2,6-xylidine
- EC Number:
- 201-758-7
- EC Name:
- 2,6-xylidine
- Cas Number:
- 87-62-7
- Molecular formula:
- C8H11N
- IUPAC Name:
- 2,6-dimethylaniline
- Test material form:
- liquid
- Details on test material:
- Name of test material: 2,6-dimethylaniline
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Xylidine
- Supplier: Ethyl Corporation,
- purity: 99.1 %
- Batch No.: E121279/01,
- no further data
Test animals
- Species:
- Drosophila melanogaster
- Strain:
- other: Canton-S and Basc
- Sex:
- male
Administration / exposure
- Route of administration:
- other: feeding and injection
- Duration of treatment / exposure:
- 3 x 2 - 3 days or single injection
Doses / concentrationsopen allclose all
- Dose / conc.:
- 100 other: mg/L (feeding)
- Dose / conc.:
- 4 000 other: mg/L (injection)
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- A total number of 70 chemicals were included in this study among them a number of test chemicals considered positive in the experiment including 4,4'-methylenedianiline 2HCI, 3-(chloromethyl) pyridine HCI, HC blue 1, maloxon, cis-dichlorodiaminoplatinum, 1,2-pro-dibromoethane, dibromomannitol, 1,2-epoxypropane, glycidol, myleran, toluene diisocyanate.
.
Examinations
- Statistics:
- For the SLRL assay, a minimum of about 5,000 chromosomes was scored from each of the treated and concurrent control groups unless the mutant frequency exceeded 1 %. If two or more lethals were recovered among the progeny of one male, a Poisson analysis [Owen, 19621 was performed to determine if these were part of a "cluster ." (A cluster is defined as a group of mutated sperm cells derived from a single mutational event occurring in a spermatogonial cell.) Clusters must be spontaneous in origin, because only meiotic and postmeiotic stages of spermatogenesis were treated. Therefore in those cases in which a male was determined to have produced a cluster the lethal and nonlethal tests for that P1 male were removed from the data. The corrected treated and control data were compared using a normal approximation to the binomial distribution, as suggested by Margolin et al. [1983]. In addition, the treated data were compared to the historical control as described by Mason et al. [1992]. For a compound to be considered mutagenic, the mutant frequency in the treated series (treated frequency must exceed 0.15 % with a P value of < 0.05, or the treated frequency exceed 0.10 % with a P value of < 0.01. If the treated frequency is between 0.10 % and 0.15 % and the P value is between 0.1 and 0.01, or if the treated frequency is higher than 0.15 % and the P value is between 0.1 and 0.05, the result is considered equivocal. All other results are considered negative. Translocation data for each treated sample were compared to the historical control data for that laboratory using a conditional binomial test [Kastenbaum and Bowman, 1970]. As a rule, at least two translocations required among about 5,000 tests in the treated series for a compound considered positive. As a comparison, the combined historical translocation rate for three laboratories, including 33.783 new tests is 4/149,946 (0.0027%).
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- yes
- Vehicle controls validity:
- valid
- Negative controls validity:
- not examined
Any other information on results incl. tables
Table 1: Summary of results
Dose of 2,6-Xylidine |
ROA |
% mortality |
% sterility |
Lethals |
Tests |
Total lethals |
Total tests |
% lethals |
||||
BR1 |
BR2 |
BR3 |
BR1 |
BR2 |
BR3 |
|||||||
100 |
feeding |
5 |
0 |
0 |
2 |
6 |
2,211 |
1,942 |
1,821 |
8 |
5,974 |
0,13 |
0 |
|
|
4 |
2 |
10 |
1,934 |
2,128 |
2,046 |
16 |
6,108 |
0,26 |
|
4000 |
injection |
14 |
0 |
4 |
1 |
3 |
1,911 |
1,793 |
1,669 |
8 |
5,373 |
0,15 |
0 |
|
|
0 |
0 |
6 |
1,999 |
1,821 |
1,811 |
6 |
5,631 |
0,11 |
One cluster of 4 lethals in treated injection experiment .
Applicant's summary and conclusion
- Conclusions:
- Negative: no increase in the frequency of sex-linked recessive lethals was found.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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