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EC number: 203-699-2 | CAS number: 109-73-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Remarks:
- Pre-guideline study performed equivalent to OECD 301 (1981)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Version / remarks:
- 1981
- Deviations:
- no
- Remarks:
- no obvious deviations based on study report
- GLP compliance:
- not specified
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- mixture of sewage, soil and natural water
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
Sludge was taken from 10 different sites in Japan (municipal STPs, industrial STPs, lakes, rivers and bays)
- Laboratory culture: yes
- Method of cultivation: fresh and old sludge was mixed at regular intervals under sufficient aeration;
about 30 minutes after ceasing the aeration the supernatant corresponding to about 1/3 of whole volume was removed.
Then the equal volume of dechlorination water was added to the remaining portion and aerated again, followed by addition
of synthetic sewage* (0.1% w/v). This procedure was repeated once every day. The culturing was carried out at 25 °C.
- Storage conditions: see above
- Storage length: see above
- Preparation of inoculum for exposure: see above
- Pretreatment: see above
- Concentration of sludge: 30 mg/L
* Synthetic sewage: each 5% (w/v) glucose, peptone and monopotassium phosphate were dissolved in dechlorinatded water,
adjusted to pH 7.0 ± 1.0 with sodium hydroxide. - Duration of test (contact time):
- 14 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: basal mineral culture medium (according to the guideline)
- Additional substrate: no
- Solubilising agent (type and concentration if used): not necessary
- Continuous darkness: yes
- Test temperature: 25 °C +/- 1°C- pH: 7.0
- pH adjusted: yes
- Aeration of dilution water: pre-filtered air was used for aeration
- Suspended solids concentration: 30 mg/L
- Replicates for test item samples (with inoculum): n= 2
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes (n=1)
- Abiotic sterile control: yes (n= 2)
- positive control (reference): yes (Aniline), n=1;
TEST SYSTEM
- Culturing apparatus: 300 mL vessels ( Coulometer: Ohkura Electric Co., Ltd)
- Measuring equipment: Closed system oxygen consumption measuring apparatus
- Details of trap for CO2 and volatile organics if used: Soda lime No.1 (extra pure reagent) - Reference substance:
- aniline
- Parameter:
- % degradation (O2 consumption)
- Value:
- 85
- Sampling time:
- 14 d
- Remarks on result:
- other: BOD / ThOD (without NH3 oxidation)
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 66
- Sampling time:
- 14 d
- Remarks on result:
- other: BOD /ThOD (including NH3 oxidation)
- Parameter:
- % degradation (TOC removal)
- Value:
- 98
- Sampling time:
- 14 d
- Parameter:
- other: HPLC analysis
- Value:
- 100
- Sampling time:
- 14 d
- Details on results:
- BOD test item flask A (300 ml; 100 mg/L test item; day 14) = BOD test item flask B (300 ml; 100 mg/L test item) = 68.4 mg;
BOD incoculum control (300 ml; day 14): 1.5 mg
Net-BOD (day 14) = 68.4 mg - 1.5 mg = 66.9 mg
ThOD_NH3: 2.63 mg O2/mg, i.e. 78.9 mg per 30 mg test item;
ThOD_NO2: 3.39 mg O2/mg, i.e. 101.7 mg per 30 mg test item;
% biodegradation (NH3) = 85%
% biodegradation (NO2) = 66%
Aniline:
BOD (day 14; 30 mg) = 69 mg; Net-BOD = 69 mg - 1.5 mg = 67.5 mg;
ThOD_NH3 Aniline: 2.405 mg O2/mg, i.e. 72.15 mg O2 per 30 mg for the test.
ThOD_NO2 Aniline: 3.01 mg O2/mg, i.e. 90.3 mg O2 per 30 mg for the test
% biodegradation (NH3) = 93.5%
% biodegradation (NO2) = 76.4%
10-day window requirement (based on plotted data within the report):
Biodegradation extent after 10 days:
BOD test item flask A (300 ml; 100 mg/L test item; day 14) = BOD test item flask B (300 ml; 100 mg/L test item) = 64.3 mg;
BOD incoculum control (300 ml; day 14): <1.5 mg
Net-BOD (day 14) = 64.3 mg - 1.5 mg = 62.8 mg
% biodegradation (NO2) = 62% - Results with reference substance:
- Within 14 days:
% biodegradation (NH3) = 93.5%
% biodegradation (NO2) = 76.4% - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Based on reliable MITI-test I (OECD 301 C) the test item is readily biodegradable, fulfilling the 10-day window requirement.
- Executive summary:
The test item n-butylamine was tested for ready biodegradability in a reliable study performed according to the MITI-I protocol (equivalent to OECD 301 C, 1981). All relevant data are given in the study report. Biodegradation extent (BOD) reached 85% and 66% without and including nitrification, respectively, within 14 days. The 10 day-window requirement was met (62% biodegradation incl. nitrification within 10 days). All validity criteria (based on OECD 301, 1992) were fulfilled.
Reference
The pass value has been reached in a 10-d window within the 28-d period of the test for all 7 replicates of the test substance.
Description of key information
Based on a reliable MITI-test I (OECD 301 C) the test item is readily biodegradable, fulfilling the 10-day window requirement.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
In the key study (CITI/NITE, 1979), the test item n-butylamine was tested for ready biodegradability in a reliable study performed according to the MITI-I protocol (equivalent to OECD 301 C, 1981). All relevant data are given in the study report. Biodegradation extent (BOD) reached 85% and 66% without and including nitrification, respectively, within 14 days. The 10 day-window requirement was met (62%
biodegradation incl. nitrification within 10 days). All validity criteria (based on OECD 301, 1992) were met.
The following supporting data are available, corroborating the result of the key study:
In a test on inherent biodegradability (Hoechst / Wellens, 1977), n-Butylamine underwent about 90 % and 100% biodegration within 2 and 5 days, respectively, based on determination of COD in the pre-guideline test equivalent to OECD 302B. This result is consitent with ready biodegradability as determined within the key study.
Further, biodegradation of n-butylamine was examined in a pre-guideline study (Yoshimura, 1980) that is comparable to the OECD TG 301-C (MITI-I) test for ready biodegradation. The BOD accounted for more than 50% of the ThOD (theoretical oxygen demand) after 5 days, and for more than 60 % after 12 days of culture.
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