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EC number: 601-101-8 | CAS number: 111497-86-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11 October 1999 - 14 October 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION- Method: Test material was stirred in demineralized water for about 48 h to prepare a stock solution (nominal concentration = 125 mg/L)
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM- Source (laboratory, culture collection): Obtained at regular intervals from SAG (Collection of algal cultures in Göttingen) and was kept in liquid culture in the Laboratory of Ecology at BASF AG Ludwigshafen
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 21-25 °C
- pH:
- 8.0-8.8
- Nominal and measured concentrations:
- Nominal concentrations: 6.25, 12.5, 25, 50 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM- Test vessel: - Material, size, headspace, fill volume: Erlenmeyer flasks (nominal volume 250 mL) plugged with gas permeable silicon sponge caps- Initial cells density: 10,000 cells/mL- No. of vessels per concentration (replicates): Triplicate- No. of vessels per control (replicates): Triplicate- No. of vessels per vehicle control (replicates): TriplicateGROWTH MEDIUM- Standard medium used: YesOTHER TEST CONDITIONS- Photoperiod: Artificial Iight, type universal white (e.g. OSRAM L 25), permanent illumination- Light intensity and quality: 60-120 µE/m2.s at a wavelength of 400-700 nmEFFECT PARAMETERS MEASURED (with observation intervals if applicable) : - Determination of cell concentrations: After 72 hours in a counting chamber (Neubauer improved) in replicate 1 of the inoculated control.- Chlorophyll measurement: In vivo chlorophyll-a-fluorescence (pulsed excitation with Iight flashes having a wavelength of 435 nm); fluorescence measured after 0, 24, 48 and 72 hours.
- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- other: EbC10
- Effect conc.:
- 23.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- other: EbC50
- Effect conc.:
- 41.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- other: EbC90
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: ErC10
- Effect conc.:
- 31 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: ErC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- other: ErC90
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Results with reference substance (positive control):
- EC50 (72 h) = 0.35 mg/L
- Reported statistics and error estimates:
- Duncan multiple range test was carried out at a 95 % significance level.
- Validity criteria fulfilled:
- yes
- Remarks:
- EC50 (72 h) of the control substance potassium dichromate was 0.35 mg/L; cell multiplication factor in the untreated control was 180 fold after 72 h; variation of the pH-values during the test was not more than 2 units
- Conclusions:
- Under the test conditions, the 72 h EbC50 of the test substance to the algae Scenedesmus subspicatus was 41.6 mg/L (nominal). The 72 h ErC50 was > 100 mg/L (nominal). The 72 h NOEC and LOEC were estimated to be 12.5 or 25 mg/L (nominal), respectively.
- Executive summary:
A study was performed to assess the acute toxicity of amine synergist to the green algae Scenedesmus subspicatus according to OECD Guideline 201, EU Method C.3 and EPA OPPTS 850.5400. No analytical determination of dose levels was carried out as the substance hydrolyses rapidly. Results were presented based on nominal concentrations. Under the test conditions, the 72 h EbC50 of the test substance was 41.6 mg/L (nominal) based on biomass. The 72 h ErC50 was > 100 mg/L (nominal) based on growth rate. The 72 h EC10 was estimated to be 31 mg/L (nominal) based on growth rate (Reuschenbach, 1999).
Reference
Description of key information
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 31 mg/L
Additional information
A study was performed to assess the acute toxicity of amine synergist to the green algae Scenedesmus subspicatus according to OECD Guideline 201, EU Method C.3 and EPA OPPTS 850.5400. No analytical determination of dose levels was carried out as the substance hydrolyses rapidly. Results were presented based on nominal concentrations. Under the test conditions, the 72 h EbC50 of the test substance was 41.6 mg/L (nominal) based on biomass. The 72 h ErC50 was > 100 mg/L (nominal) based on growth rate. The 72 h EC10 was estimated to be 31 mg/L (nominal) based on growth rate (Reuschenbach, 1999).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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