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EC number: 205-205-0 | CAS number: 135-61-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-11-06 to 2009-11-13
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: study performed according to current guideline and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-hydroxy-2'-methyl-2-naphthanilide
- EC Number:
- 205-205-0
- EC Name:
- 3-hydroxy-2'-methyl-2-naphthanilide
- Cas Number:
- 135-61-5
- Molecular formula:
- C18H15NO2
- IUPAC Name:
- 3-hydroxy-N-(2-methylphenyl)naphthalene-2-carboxamide
- Reference substance name:
- Naphtol AS-D
- IUPAC Name:
- Naphtol AS-D
- Details on test material:
- - Name of test material (as cited in study report): Naphtol AS-D
- Molecular formula: C18H15NO2
- Molecular weight: 277.33 g/mol
- Physical state: Solid, beige powder
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: The limit concentration and the control were analytically verified at the beginning and the end of the test via HPLC-DAD analysis.
- Sampling method: Samples at test start and end were taken directly from the test replicates.
- Sample storage conditions before analysis: All samples were stored at 6 ± 2 °C until start of analysis, if necessary.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A saturated solution* was prepared. A dispersion of 100 mg/L prepared in dilution water was shaken with 20 rpm for 48 h at ambient temperature and centrifuged at 10000 rpm for 10 min.
- Eluate: Dilution water
- Differential loading: The saturated solution was tested in a limit test corresponding to a
geometric mean measured concentration of 0.711 mg/L.
- Controls: Six replicates without test item were tested under the same test conditions as the test replicates.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Desmodesmus subspicatus Chodat
- Strain: SAG 86.81
- Source (laboratory, culture collection): SAG Pflanzenphysiologisches Institut der Universitaet Goettingen, Nikolausberger Weg 18, D-37073 Goettingen, Germany
- Age of inoculum (at test initiation): 4 days
- Method of cultivation: Fresh stocks were prepared from Z-Agar. Light intensity amounted 35 - 70 µE x m-2 x s-1 for 24 h per day.
ACCLIMATION
- Culturing media and conditions (same as test or not): Same as test
- Any deformed or abnormal cells observed: No
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- Not measured
- Test temperature:
- Mean: 22 °C, Min: 21 °C, Max: 23 °C
- pH:
- Geometric mean measured test item concentration pH-value
[mg/L] Start; 0 h End; 72 h
0.711 8.02 8.53
Control 8.12 8.54 - Dissolved oxygen:
- Not measured
- Salinity:
- Not measured, freshwater
- Nominal and measured concentrations:
- Please refer to "any other information on materials and methods incl. tables"
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: sterile 250 mL Erlenmeyer flasks, test volume 100 mL
- Aeration: Test containers were placed on a rotary shaker and oscillated at appr. 70 rpm
- Type of flow-through (e.g. peristaltic or proportional diluter): None
- Renewal rate of test solution (frequency/flow rate): One application at test start
- Initial cells density: 5074 cells/mL
- Control end cells density: Mean 378432 cells/mL
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): None
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water, composition acc. to the guideline
- Hardness: 0.24 mmol Ca+Mg/L
- pH-value: 8.1 +/- 0.2
- Culture medium: Nutrient Medium Z acc. to LÜTTGE et al. (1994)
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: 24 h
- Light intensity and quality: 60 - 120 µE x m-2 x s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter] Fluorimeter, via Chlorophyll-a fluorescence (excitation at 436 nm, emission at 685 nm), cell concentrations were related to all cell density values acc. to a calibration curve.
TEST CONCENTRATIONS
- Range finding study: Yes
Results of the Range Finding Test (0 – 72 h)
Saturated solution [%] Specific Growth Rate Inhibition [%] Yield Inhibition [%]
100 - 6 - 34
10 - 5 - 27
1 - 2 - 13 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.711 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks:
- and yield inhibition
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test):
- Unusual cell shape: No
- Colour differences: No
- Flocculation: No
- Adherence to test vessels: No
- Aggregation of algal cells: No
- Other: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50:
Rate-related inhibition: 0.600 (CI 0.545 - 0.653) mg/L
Yield Inhibition: 0.280 (CI 0.266 - 0.299) mg/L - Reported statistics and error estimates:
- The NOEC and LOEC were determined by calculation of statistical significance of growth rates and yield.
t-test was carried out for the determination of statistically significant differences compared to control replicates. When running a t-test a Normality Test and an Equal Variance Test were done first. P-values for both Normality and Equal Variance Tests were 0.05. The a-value was a=0.05. Detection of statistically significant differences were considered negligible when inhibition values were ≤ 10 %. These findings were marked biological not significant.
Any other information on results incl. tables
NOEC, LOEC and EC-Values of Naphtol AS-D (0-72 h)
based on the geometric mean measured test item concentration [mg/L]
EC-Values |
Rate-related inhibition |
NOEC |
0.711 |
LOEC |
> 0.711 |
ErC10 |
> 0.711 |
ErC20 |
> 0.711 |
ErC50 |
> 0.711 |
Yield inhibition |
|
NOEC |
0.711 |
LOEC |
> 0.711 |
EyC10 |
> 0.711 |
EyC20 |
> 0.711 |
EyC50 |
> 0.711 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study the effects of the test item Naphtol AS-D on the growth of the freshwater green alga Desmodesmus subspicatus were tested. No inhibiting effects on specific growth rate and yield were found in the limit concentration of 0.711 mg/L. The EC50-values for specific growth rate inhibition (ErC50) and inhibition of yield (EyC50) after 72 h were > 0.711 mg/L.
All effect levels are given based on the geometric mean measured test item concentration. - Executive summary:
The toxicity of Naphtol AS-D (Batch no.: Changshu Huayi, Batch 040 267) to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD 201.
The concentration of Naphtol AS-D was determined at test start and end via HPLC-DAD analysis. The measured concentration at test end was 41 % of the initially measured test item concentration. All effect levels are given based on the geometric mean measured test item concentration.NOEC, LOEC and EC-Values of Naphtol AS-D (0-72 h)
based on the geometric mean measured test item concentration [mg/L]
EC-Values
Rate-related inhibition
NOEC
0.711
LOEC
> 0.711
ErC10
> 0.711
ErC20
> 0.711
ErC50
> 0.711
Yield inhibition
NOEC
0.711
LOEC
> 0.711
EyC10
> 0.711
EyC20
> 0.711
EyC50
> 0.711
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