Registration Dossier
Registration Dossier
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EC number: 212-110-8 | CAS number: 763-32-6
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study. Scientifically acceptable
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- no
Test material
- Reference substance name:
- 3-methylbut-3-en-1-ol
- EC Number:
- 212-110-8
- EC Name:
- 3-methylbut-3-en-1-ol
- Cas Number:
- 763-32-6
- Molecular formula:
- C5H10O
- IUPAC Name:
- 3-methylbut-3-en-1-ol
- Details on test material:
- - Name of test material (as cited in study report): 3-Methylbuten-3-ol-1
no further details on the test substance characteristics given.
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 136 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST SYSTEM
- Culturing apparatus: Sapromat
- Number of culture flasks/concentration: 3
CONTROL AND BLANK SYSTEM
Number of replicates: Test substance: 3 (6 other replicates contained another test substance) Blank control: 2 Positive control: 1
Test substance: 85 and 136 mg/l, corresponds to 93 and 58 mg/l DOC, respectively. Tap water: 240 ml Total liquid volume: 250 ml Inoculum (750 mg/l stock solution): 10 ml Inoculum concentration in the test: 30 mg/l dry weight Incubation: at room temperature (22 +/- 2 °C) on a magnetic stirrer. Measurements: DOC-content in the controls and each treatment group at daily intervals from day 0 to day 28
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 70 - 80
- Sampling time:
- 28 d
- Details on results:
- Kinetic of test substance (in %):
< 10 after 3 day(s)
ca. 30 after 4 day(s)
ca. 61 after 14 day(s)
ca. 68 after 21 day(s)
ca. 71 after 28 day(s)
BOD5 / COD results
- Results with reference substance:
- Kinetic of control substance (in %):
after 4 day(s)
after 28 day(s)
Applicant's summary and conclusion
- Interpretation of results:
- readily biodegradable
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