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EC number: 203-475-4 | CAS number: 107-25-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Repeated dose toxicity: inhalation
Administrative data
- Endpoint:
- short-term repeated dose toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 412 (Subacute Inhalation Toxicity: 28-Day Study)
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- (CIVO TNO)
- Limit test:
- no
Test material
- Reference substance name:
- Methyl vinyl ether
- EC Number:
- 203-475-4
- EC Name:
- Methyl vinyl ether
- Cas Number:
- 107-25-5
- Molecular formula:
- C3H6O
- IUPAC Name:
- methoxyethene
- Details on test material:
- Methyl vinyl ether, obtained from BASF AG;
purity 99.7%
impurities: ethylvinylether 0.25%, methanol < 0 .1% , and water 0.05%
The stability of the test material was confirmed by reanalysis
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- breeder: Winkelmann, Versuchstierzucht GmbH & Co. KG, Borchen, Germany)
Mean body weights: 140 g and 121 g for males and females, respectively, at start of the study.
Feeding: the Institute's standard diet ad libitum, not during exposure
Drinking water: tap water ad libitum, not during exposure
acclimatisation: 10 days
no further data
Administration / exposure
- Route of administration:
- inhalation
- Type of inhalation exposure:
- whole body
- Vehicle:
- other: air
- Remarks on MMAD:
- MMAD / GSD: not applicable
- Details on inhalation exposure:
- Whole-body exposure in glass cylinders (length 0.90 m, diameter 15 cm). Temperature during exposure: 21 °C. Relative humidity during exposure: 43 - 51%. Test atmosphere generation: mixing an adjustable flow of methyl vinyl ether vapour with the main air flow to the glass cylinders.
- Analytical verification of doses or concentrations:
- yes
- Details on analytical verification of doses or concentrations:
- Exposure levels: analyses of the test atmospheres were performed by infra-red spectroscopy at least four times per hour. See Table below.
- Duration of treatment / exposure:
- 28 days
- Frequency of treatment:
- 6 hrs/day, 5 days/week
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
0, 500, 3500, 25000 ppm (0, 1.2, 8.4, or 60 mg/L)
Basis:
nominal conc.
- Remarks:
- Doses / Concentrations:
0, 500, 3480, 25080 ppm
Basis:
analytical conc.
- No. of animals per sex per dose:
- 5
- Control animals:
- yes, concurrent vehicle
- Details on study design:
- Post-exposure period: none
- Positive control:
- no
Examinations
- Observations and examinations performed and frequency:
- clinical signs: twice a day (once before the start of the exposure and once after the exposure); during the weekends once a day.
Body weights: recorded just prior to the start of the study and then weekly.
Haematology: red and white blood cells, haemoglobin, diff. white blood cell counts, haematocrit, prothrombin time, reticulocyte counts, MCV, MCH, MCHC. Blood samling: tip of the tail at the end of the exposure period.
Clinical chemistry: albumin, GOT (or ASAT), alkaline phosphatase, GPT (or ALAT), gamma-GT, urea, total protein, creatinine, total bilirubin, Ca, K, Na, inorganic phosphate, Cl, glucose; blood samples of abdominal aorta at autopsy. - Sacrifice and pathology:
- Pathology
Animals killed at day 28, autopsied and examined for gross pathological changes.
Organ weights of adrenals, brain, heart, kidneys, liver, lungs with mediastinal lymph nodes, trachea and larynx, spleen, testes measured
Histopathological examination was done on all organs and tissues collected of all rats (adrenals, brain, heart, kidneys, liver, lungs with mediastinal lymph nodes, trachea and larynx, spleen, testes, sternum, nose). - Other examinations:
- no
- Statistics:
- Statistical data analysis included analysis of co-variance followed by the Dunnett test (body weights), Fischer Exact test (incidences of histopathological changes), and analysis of variance and Dunnett test (organ weights, hematological and biochemical data).
Level of significance: p<=0.05
Results and discussion
Results of examinations
- Clinical signs:
- effects observed, treatment-related
- Mortality:
- mortality observed, treatment-related
- Body weight and weight changes:
- effects observed, treatment-related
- Food consumption and compound intake (if feeding study):
- not specified
- Food efficiency:
- not specified
- Water consumption and compound intake (if drinking water study):
- not specified
- Ophthalmological findings:
- not specified
- Haematological findings:
- no effects observed
- Clinical biochemistry findings:
- effects observed, treatment-related
- Urinalysis findings:
- not specified
- Behaviour (functional findings):
- not specified
- Organ weight findings including organ / body weight ratios:
- effects observed, treatment-related
- Gross pathological findings:
- no effects observed
- Histopathological findings: non-neoplastic:
- effects observed, treatment-related
- Histopathological findings: neoplastic:
- no effects observed
- Details on results:
- Mortalities: none in either group.
Animals exposed to 25000 ppm showed transient restlessness and pilo-erection.
Body weights: Statistically significantly (p <= 0.05) reduced body weights in high-dose males throughout the study. Reduced weight in mid-dose males and high-dose females gaining statistical significance at week 1 (males) and week 3 (females).
Hematology (all data means +-SEM): The number of white blood cells was reduced in males, significantly at 500 and 3500 ppm (16.5+-1.5, 11.4+-0.6, 12.2+-1.1, 13.1+-0.8, respectively); the effect was not dose-related. Prothrombin times were increased (not significant) compared to controls in all exposed males and in high-dose females.
Blood chemistry: Total protein was decreased in all male dose groups (p <= 0.01; 56.7+-0.5, 53.8+-0.3, 53.4+-0.2, 52.8+-0.8 g/L, respectively); GOT was decreased in the high-dose group (p < 0.01; 51.1+-0.7 versus 60.3+-2.0 U/L in control).
Organ weights: absolute, but not relative, spleen and lung weights were decreased (p <= 0.05) in male groups at the intermediate and the high-dose-level. Relative liver weight of the high-dose female group was significantly (p <0.05) increased. It tended to be higher in females than in males. Testes weights unaffected at all dose levels.
Gross pathology: no treatment-related findings noted.
Histopathology: decrease of the number of olfactory epithelial cells in high-dose groups. The effect was more pronounced in females than in males. No incidence of reduced spermatogenesis, interstitial edema, or multinucleated giant-cells in any treatment group; incidence in control group animals 1/5 each.
Effect levels
open allclose all
- Dose descriptor:
- NOAEC
- Effect level:
- 3 500 ppm
- Sex:
- female
- Basis for effect level:
- other: (8.4 mg/L)
- Dose descriptor:
- LOAEC
- Effect level:
- 25 000 ppm
- Sex:
- female
- Basis for effect level:
- other: reduced body weight, clinical signs and local effects (atrophy of olfactory epithelium)
- Dose descriptor:
- LOAEC
- Effect level:
- 500 ppm
- Sex:
- male
- Basis for effect level:
- other: (1.2 mg/L); lowest dose tested; increased prothrombin time, decrease in total protein
Target system / organ toxicity
- Critical effects observed:
- not specified
Any other information on results incl. tables
No data available on historical control data for haematology and clinical chemistry of this lab and this strain.
Applicant's summary and conclusion
- Conclusions:
- The NOAEC was 3500 ppm for females, local effects on the olfactory epithelium, clinical signs and reduced body weight were detected at 25000 ppm.
No NOAEC could be established for males because of the
increased prothrombin time and the decreases in total
protein in all male dose groups. - Executive summary:
GLP guideline study.
A 4 -week inhalation toxicity study in rats was carried out with methyl-vinyl ether. Four groups of 5 male and 5 female Wistar rats each, were exposed (whole body) to target concentrations of 0, 500, 3500, 25000 ppm (0, 1.2, 8.4, or 60 mg/L) for 6 hours a day, five days a week during a period of four weeks. Clinical signs, body weights, weekly food consumption, haematological and biochemical parameters, gross- and microscopic pathology were used to examine the toxicological potency of the test substance. No NOAEC was identified for male rats; increased prothrombin time and decrease in total protein was found in all treatment groups. Therefore a 2nd study in male rats was performed (see next study record). The NOAEC for females was 3500 ppm; at 25000 ppm local effects on the olfactory epithelium, clinical signs and reduced body weight were found.
Conclusion: The NOAEC was 3500 ppm for females, local effects on the olfactory epithelium, clinical signs and reduced body weight were detected at 25000 ppm. No NOAEC could be established for males because of the increased prothrombin time and the decreases in total protein in all male dose groups.
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