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EC number: 247-045-4 | CAS number: 25498-49-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 1983
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP study, equivalent to OECD Guideline no 473.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
- Version / remarks:
- for the absence of S9positive control was not used.
- Deviations:
- yes
- Remarks:
- for the absence of S9, positive control was not used.
- GLP compliance:
- yes
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- 1-methoxypropan-2-ol
- EC Number:
- 203-539-1
- EC Name:
- 1-methoxypropan-2-ol
- Cas Number:
- 107-98-2
- IUPAC Name:
- 1-methoxypropan-2-ol
- Details on test material:
- - Name of test material (as cited in study report): Dowanol PM
- Physical state: clear liquid
Constituent 1
Method
- Target gene:
- Induction of chromosomal damage (clastogenicity).
Species / strain
- Species / strain / cell type:
- Chinese hamster Ovary (CHO)
- Details on mammalian cell type (if applicable):
- - Type and identity of media: Mc Conys 5A medium supplemented with 15% fetal calf serum and antibiotic ( Penicillin and streptomycin)
- Periodically checked for Mycoplasma contamination: no data
- Periodically checked for karyotype stability: no data
- Periodically "cleansed" against high spontaneous background: no data - Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 liver homogenate prepared from AROCLOR 1254 treated Sprague-Dawley rats
- Test concentrations with justification for top dose:
- 1.25, 2.5, 5.0, 10.0 mg/ml
- Vehicle / solvent:
- the test material was dissolved directly in the culture medium
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- cyclophosphamide
- Evaluation criteria:
- Structural chromosomal abnormalities that were scored included chromatid and chromosome gaps, chromatid breaks and exchanges, chromosome breaks and exchanges, and chromosomal disintegration. Chromatid and chromosome gaps were not included in the number of total aberrations.
The final interpretation of biological significance of the cytogenetic responses was based on both statistical outcome and sound scientific judgement.
Results and discussion
Test results
- Species / strain:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: PM was not toxic to CHO cells up to 5 mg/ml and reduced survival to approximatale 50% at 10 mg/ml.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- Cultures treated with 500 ug/ml cyclophosphamide served as positive controls for activation assays. Negative control cultures were treated with culture medium (the solvent used to dissolve the test material). PM was not toxic to CHO cells up to 5 mg/ml and reduced survival to approximatale 50% at 10 mg/ml.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Results are shown in the table below:
Dose Level With/without Cytotoxicity Aberrations
(ug/ml) S-9
------------------------------------------------------
0 Dowanol PM +/- Negative Negative
1.25 Dowanol PM +/- Negative Negative
2.5 Dowanol P +/- Negative Negative
5.0 Dowanol PM +/- Negative Negative
10 Dowanol PM +/ - slight toxic Negative
0.5 CP + no data Positive
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Dowanol PM was non-clastogenic to cultured CHO cells under the experimental conditions used. - Executive summary:
Dowanol Propylene glyocl methyl ether (Dowanol PM) was evaluated in an vitro chromosomal aberration assay utilizing Chinese hamster ovary (CHO) cells. The clastogenicity of the test material was assessed in the absence and presence of an externally supplied metabolic activation (S-9) system at dose levels of 1.25, 2.5, 5.0, 10.0 mg/ml of Dowanol Pm. prior to main expiriment a cytotoxicity assay was performed for the dose range finding. Cultures treated with 500 µg/ml cyclophosphamide served as positive controls for the activation assays. Negative control cultures were treated with culture medium (the solvent used to dissolve the test material). There were no statistically significant increases in the frequencies of chromosomal aberrations in cultures treated with the test material either in the absence or presence of of S-9 as compared to the negative conrol cultures. The positive control chemicals induced the epxected increases in aberration frequencies. Hence, under the experimental conditions used,
Dowanol PM was considered to be non-clastogenic to CHO cells in culture.
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