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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Currently viewing:

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Adequacy of study:
other information

Data source

Reference
Reference Type:
other: Body responsible for the test
Title:
Unnamed

Materials and methods

Test guideline
Qualifier:
according to guideline
Principles of method if other than guideline:
European Economic Community (EEC), EEC Directive 92/69, Part
C: Methods for the determination of ecotoxicity,
Publication No. L383, C-3: "Algal Inhibition Test" adopted
December 1992.

Organization for Economic Co-operation and Development
(OECD), OECD guideline for Testing of Chemicals, guideline
No. 201: "Algae, Growth Inhibition Test", Adopted June 7,
1984.
GLP compliance:
yes

Test solutions

Details on test solutions:
Identity and concentration of auxiliary solvent for dispersal: Preparation of test solutions started with a stock solution
of 100 mg/l applying 30 minutes of magnetic stirring. For
the range-finding test the resulting dispersion was filtered
through a paper filter Schleicher and Schuell 604, (ca. 5
um). For both the limit test and the final test the
resulting dispersions were filtered through a glass fibre
pre-filter (Schleicher and Schuell GF 92) to remove the
larger undissolved test substance particles and subsequently
through a membrane filter (Schleicher and Schuell RC55,
0.45um). The lower test concentrations for the range-finding
test and the final test were prepared by subsequent
dilutions of the filtrate in test medium. The final test
solutions were all clear and colourless.

Test organisms

Test organisms (species):
other: Selenastrum capricornutum, strain: NIVA CHL1

Study design

Limit test:
no
Total exposure duration:
72 h

Test conditions

Hardness:
24 mg CaCO3/L

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
ca. 0.57 mg/L
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 0.73 mg/L
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.34 mg/L
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.15 mg/L
Basis for effect:
biomass

Applicant's summary and conclusion