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EC number: 265-111-0 | CAS number: 64742-11-6 A complex combination of hydrocarbons obtained as the extract from a solvent extraction process. It consists predominantly of aromatic hydrocarbons having carbon numbers predominantly in the range of C20 through C50. This stream is likely to contain 5 wt. % or more of 4- to 6-membered condensed ring aromatic hydrocarbons.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 1985-11-26 to 1986-02-05
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study is classified as reliable without restriction because it was conducted according to or similar to guideline study OECD TG 476.
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
- Reference Type:
- publication
- Title:
- Laboratory procedure for assessing specific locus mutations at the TK locus in cultured L5178Y mouse lymphoma cells
- Author:
- Clive, D. and Spector, J.F.S.
- Year:
- 1 975
- Bibliographic source:
- Mutation Res., vol. 31, pp. 17-29
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- GLP compliance:
- yes
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- 64742-05-8
- IUPAC Name:
- 64742-05-8
- Reference substance name:
- Light paraffinic distillate solvent extract
- IUPAC Name:
- Light paraffinic distillate solvent extract
- Test material form:
- liquid: viscous
- Details on test material:
- - Name of test material (as cited in study report): API 83-16
- Substance type: Petroleum
- Physical state: Clear dark brown liquid
- Viscosity, SSU: 67.2 at 100°F
- API Gravity: 16.7
- Flash Point: (°F) 335
- Pour Point (°F): 20
- Aniline Point (°F): 95.5
- Sulfur, Wt %: 2.64
- Composition of test material ASTM D-2007, Wt. %:
Saturates: 29.2
Aromatics 68.6
Constituent 1
Constituent 2
Method
Species / strain
- Species / strain / cell type:
- mouse lymphoma L5178Y cells
- Details on mammalian cell type (if applicable):
- - Type and identity of media: RPMI-1640 medium supplemented with pluronic solution, L-glutamine, sodium pyruvate, antibiotics, and horse serum (10% by volume)
- Properly maintained: yes
- Periodically checked for Mycoplasma contamination: yes
- Periodically checked for karyotype stability: yes/no No data
- Periodically "cleansed" against high spontaneous background: yes/no No data - Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Test concentrations with justification for top dose:
- 25, 50, 75, 100, 150 and 200 µL/mL without activation and at 12.5, 25, 50, 75, 100 and 150 µL/mL with activation
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Ethanol
- Justification for choice of solvent/vehicle: More compatible with cell viability than acetone.
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- ethanol
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- ethylmethanesulphonate
- Remarks:
- Migrated to IUCLID6: for nonactivation studies
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: In medium
DURATION
- Preincubation period: 10 to 14 days
- Expression time (cells in growth medium): 2 days
SELECTION AGENT (mutation assays): 3µg/mL 5-trifluorothymidine (TFT)
NUMBER OF REPLICATIONS: not reported
NUMBER OF CELLS EVALUATED: not reported - Evaluation criteria:
- The minimum criterion considered necessary to demonstrate mutagenesis for any given treatment will be a mutant frequency that is at least 150% of the concurrent background frequency plus 10 x10 e-6. The background frequency is defined as the average mutant frequency of the solvent negative controls.
Results and discussion
Test results
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- not examined
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- not examined
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive with metabolic activation
positive without metabolic activation
In the absence of metabolic activation, a dose-response relationship was observed from 75 µL/mL to 200 µL/mL DAE with decreasing relative growth percentages and increasing mutant frequency (in 10E-6 units). The minimum criterion for mutagenesis in this assay was a mutant frequency exceeding 67.9x10-6. Concentrations of 150 µL/mL and 200 µL/mL induced mutant frequencies that exceeded the minimum criterion. Hence the tested material was considered mutagenic without activation.
In the presence of metabolic activation, a dose-response relationship was observed from 75 µL/mL to 150 µL/mL DAE with decreasing relative growth percentages and increasing mutant frequency (in 10E-6 units). The minimum criterion for mutagenesis in this assay was a mutant frequency exceeding 73.5x10-6. Treatments from 25 µL/mL to 150 µL/mL induced increases in the mutant frequency, which ranged from 1.8-fold to 3.2-fold above the minimum criterion. There was a general trend toward higher mutant frequencies at higher concentrations of the test material. The tested material was therefore considered mutagenic with activation.
Light paraffinic distillate solvent extract is therefore mutagenic with and without activation under the conditions specified in this assay. - Executive summary:
In a mammalian cell gene mutation assay mouse lymphoma L5178Y cells cultured in vitro were exposed to light paraffinic distillate solvent extract in ethanol at concentrations of 25, 50, 75, 100, 150 and 200 µL/mL without activation and at 12.5, 25, 50, 75, 100 and 150 µL/mL with activation.
In the absence of metabolic activation, a dose-response relationship was observed from 75 µL/mL to 200 µL/mL DAE with decreasing relative growth percentages (i.e., 119.7% to 2.2%) and increasing mutant frequency (in 10E-6 units). The minimum criterion for mutagenesis in this assay was a mutant frequency exceeding 67.9x10-6. Concentrations of 150 µL/mL and 200 µL/mL induced mutant frequencies that exceeded the minimum criterion. Hence the tested material was considered mutagenic without activation. In the presence of metabolic activation, a dose-response relationship was observed from 75 µL/mL to 150 µL/mL DAE with decreasing relative growth percentages and increasing mutant frequency (in 10E-6 units). The minimum criterion for mutagenesis in this assay was a mutant frequency exceeding 73.5x10-6. Treatments from 25 mL/mL to 150 mL/mL induced increases in the mutant frequency, which ranged from 1.8-fold to 3.2-fold above the minimum criterion.
There was a general trend toward higher mutant frequencies at higher concentrations of the test material. The tested material was therefore considered mutagenic with activation. Light paraffinic distillate solvent extract is therefore mutagenic with and without activation under the conditions specified in this assay.
This study received a Klimisch score of one and is considered reliable without restriction because it was conducted according to or similar to guideline study OECD TG 476.
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