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EC number: 203-148-6 | CAS number: 103-82-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Qualifier:
- according to guideline
- Guideline:
- other:
- Principles of method if other than guideline:
- Data is from publication
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Details on test solutions:
- The carrier of fluoranthene and its metabolites was a recommended mineral medium, composed for the test as follows (mg/l):
NH4Cl ¼ 15; MgCl2 6H2O ¼ 12; CaCl2 2H2O ¼ 18; MgSO4 7H2O ¼ 15; KH2PO4 ¼ 1.6; NaHCO3 ¼ 50 and (lg/l): FeCl3 6H2O ¼ 80; Na2EDTA 2H2O ¼ 100;
H3BO3 ¼ 185; MnCl2 4H2O ¼ 415; ZnCl2 ¼ 3; CoCl2
6H2O ¼ 1.5; CuCl2 2H2O ¼ 0.01 and Na2MoO4
2H2O ¼ 7. The pH was adjusted to 8.30.2. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Source: National Institute of Biology (NIB, Ljubljana, Slovenia).
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
- Details on test conditions:
- Method: In mineral medium; algal culture incubated with geometric dilution of 10 concentrations of title comp. for 7 days; at day 0 and after 7 days algal cells counted, growth rate with interpolation used.
Further Details : 12 h day/night photoperiod with blue and red wavelengths; for each concentration four replicate samples and one blank sample prepared - Reference substance (positive control):
- not specified
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 177 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth inhibition
- Conclusions:
- The effective concentration (EC50) value of test material in aquatic green algae [Scenedesmus subspicatus] on the basis of growth inhibition effect was found to be 177mg/L.Thus,considering the CLP Criteria for aquatic classification of the substance , it isconcluded that test material does not exhibit toxicity to aquatic green algae [Scenedesmus subspicatus].
- Executive summary:
The effective concentration (EC50) value of test material in aquatic green algae [Scenedesmus subspicatus] on the basis of growth inhibition effect was found to be 177 mg/L.Thus,considering the CLP Criteria for aquatic classification of the substance , it isconcluded that test material does not exhibit toxicity to aquatic green algae [Scenedesmus subspicatus].
Reference
Description of key information
Toxicity to aquatic algae and cyanobacteria:
The effective concentration (EC50) value of test material in aquatic green algae [Scenedesmus subspicatus] on the basis of growth inhibition effect was found to be 177 mg/L.Thus,considering the CLP Criteria for aquatic classification of the substance , it isconcluded that test material does not exhibit toxicity to aquatic green algae [Scenedesmus subspicatus].
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 177 mg/L
Additional information
Toxicity to aquatic algae and cyanobacteria:
The effect of test material on aquatic algae was described in a peer reviewed journal , the effective concentration (EC50) value of test material in aquatic green algae [Scenedesmus subspicatus] on the basis of growth inhibition effect was found to be 177 mg/L.Thus,considering the CLP Criteria for aquatic classification of the substance , it isconcluded that test material does not exhibit toxicity to aquatic green algae [Scenedesmus subspicatus].
The above data was further supported by data for structurally similar read across substance , the effect of test substance was studied on the growth of fresh water green algaChlorella vulgaris.The study was conducted following OECD guideline 201- Alga growth inhibition test.The test substance , was prepared by adding 106.25 mg of test substance in 250 ml of BBM to get the final concentration of 425 mg/L. The sock solution was ultrasonically agitated for 30 minutes to obtain a homogenous solution for the experiment. The remaining test solutions were prepared by dilution from the above stock solution, under aseptic condition. To have a better growth and visibility of cells, the initial cell density of the culture was kept 1 X 104 cells/ml. Care was taken to have a homogeneous solution for the experiment.
The test concentration chosen for the study were 4.32mg/l, 10.88 mg/l, 27.2mg/l, 68mg/l, 170mg/l and 425mg/l were prepared using stock solution of the test substance using de-ionized water. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of test substance. EC50 calculated from equation through probit analysis was observed to be 138.96 mg/L.
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