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EC number: 207-330-6 | CAS number: 462-95-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February 17-November 11, 1986
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to OECD guideline 403 and following GLP principles
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
Test material
- Reference substance name:
- Diethoxymethane
- EC Number:
- 207-330-6
- EC Name:
- Diethoxymethane
- Cas Number:
- 462-95-3
- Molecular formula:
- C5H12O2
- IUPAC Name:
- diethoxymethane
- Details on test material:
- - Name of test material (as cited in study report): diethoxymethane
- Analytical purity: 99.9%
- Impurities (identity and concentrations): pyrogallol (10 ppm)
- Lot/batch No.: X-19085-9
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: CRL:CD(SD)BR
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Laboratories, MA
- Age at study initiation:
- Weight at study initiation: 212-250 g (males) and 192-224 g (females)
- Fasting period before study:
- Housing: Animals were singly housed in multicompartmented stainless steel mesh cages
- Diet (e.g. ad libitum): Certified feed (Agway Prolab Animal Diet (RMH 3000, pellets)) available ad lib
- Water (e.g. ad libitum): Water (Monroe County Water Authority) ad lib
- Acclimation period:
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21
- Humidity (%): 36-43
- Air changes (per hr): 15-20
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: From: To:
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- clean air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
Exposures were conducted in 20 L glass bell jar inhalation chambers contained in a hood. Chambers were maintained under slight positive pressure and at 15-20 air changes per hour. Atmospheres were produced by passing metered dried oil-free compressed air over the surface of the test material contained in a 500 ml round bottom flask. Controls were treated identically to the test groups, except that exposure was to filtered air only. Temperature was determined hourly and nominal chamber concentration for the exposure was calculated.
TEST ATMOSPHERE
- Brief description of analytical method used: Chamber vapor concentrations were determined at least once per hour by a Miran IA infrared analyser equipped for automated sampling and analysis. On the morning and afternoon of the exposure, concentration of background nongaseous material was measured in the high concentration (20000 ppm) chamber relative to the control chamber in order to insure that exposure were to vapor and not aerosol.
- Samples taken from breathing zone: yes - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 6 h
- Concentrations:
- 0, 5000, 10000 and 20000 ppm
- No. of animals per sex per dose:
- 5
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: before and after exposure and twice daily thereafter for observations; on days 0, 3, 7, 10, 14 and at necropsy for body weight
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, histopathology - Statistics:
- One-way analysis of variance (ANOVA), Bartlett's test and Duncan's multiple range test using a P value ≤ 0.05 to indicate statistical significance. LC50 and its 95% CL were estimated by probit analysis using SAS Institute Inc. Version 5, 1985 (Cary, NC)
Results and discussion
- Preliminary study:
- Not relevant
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- 6 643 ppm
- Based on:
- test mat.
- 95% CL:
- 5 117 - 8 476
- Exp. duration:
- 6 h
- Mortality:
- Males: death of 5/5 at 20000 ppm (within 3 hours into exposure); death of 5/5 at 10000 ppm (during the 6-hour exposure); no death at 5000 ppm.
Females: death of 5/5 at 20000 ppm (within 5 hours into exposure); death of 4/5 at 10000 ppm (during the 6-hour exposure); death of 2/5 at 5000 ppm (shortly after exposure). - Clinical signs:
- other: During exposure: wobbly gait, lethargy and narcosis. Following exposure: For males: lethargy and gait disturbance at 5000 ppm. Corneal opacy for 1/5 on Day 7 to the end. For females: lethargy at 5000 ppm; narcosis following exposure at 10000 ppm All the
- Body weight:
- BW gain of all the surviving animals was comparable to control values.
- Gross pathology:
- Treatment-related changes were observed in the lungs and livers of all 3 exposure groups in females and the high- and middle-exposure groups of males. They were characterized as the incomplete collapse of lungs on thoratomy and enlarged livers. Failure of the lungs to collapse upon thoracotomy was probably caused by edema. The cause of the enlarged livers was not determined. Tissues were not collected for microscopic evaluation.
Inflammatory eye changes were observed in 4 female rats from the high dose group. Microscopic evaluation of the affected eyes revealed unilateral or bilateral congestion and edema of the ciliary body and hemorrhage of the choroid. - Other findings:
- Aerosol measurements:
Measurements obtained with the Roco Model 225 Aerosol Particle Conter (HIAC/Royco Instruments Division, Pacific Scientific, Menlo Park, CA) revealed that an aerosol was not present.
Any other information on results incl. tables
Nominal concentrations differed from the analytical ones:
Concentration | Low (ppm) | Intermediate (ppm) | High (ppm) |
Nominal | 5000 | 10000 | 20000 |
Analytical (males) | 5357 ± 413 | 9802 ± 814 | 20029 ± 219 |
Analytical (females) | 5060 ± 181 | 9393 ± 840 | 16802 ± 4054 |
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The 6-hour LC50 value for males and females rats combined is 6643 ppm with a 95% confidence interval of 5117 to 8476 ppm in the acute inhalation toxicity study of diethoxymethane.
- Executive summary:
An acute inhalation toxicity test was performed in the rat according to OECD guideline 401 and GLP compliance.
Groups of 5 male and 5 female rats were exposed to target vapor concentrations of 20000, 10000, 5000 or 0 ppm diethoxymethane (DEM) for 6 hours and then held for 14 days of observation. DEm caused lethargy, gait disturbance, narcosis and death in all rats, within 5 hours during exposure to 20000 ppm, and by 6 hours in all but one rat exposed to 10000 ppm. All the males and females survived exposure to 5000 ppm but 2 females died shorthly thereafter. Treatment-related gross changes in animals dying spontaneously were incomplete collapse of lungs and enlarged livers. No microscopic examinations were conducted. Other than transient narcosis, lethargy or gait disturbance following exposure, there were no compound-related clinical signs of toxicity or changes in bw gain in females which survived exposure to 5000 ppm. No treatment-related gross changes were seen in the surviving female exposed to 10000 ppm or in males exposed to 5000 ppm. Incomplete collapse of the lungs and enlarged livers were seen in females surviving exposure to 5000 ppm. Except for the microscopic examination of eyes which showed incidental inflammatory changes in 4 females exposed to 20000 ppm, no other microscopic examonations were performed. The lung and liver are sites of toxicity at these high concentrations. A no-effect level of toxicity was not determined.
The 6-hour LC50 value for males and females rats combined is 6643 ppm with a 95% confidence interval of 5117 to 8476 ppm in the acute inhalation toxicity study of DEM.
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