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EC number: 203-544-9 | CAS number: 108-03-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation
- Remarks:
- in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1981
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: No data on GLP, comparable to a guideline study
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- According to Landsteiner and Jacobs procedure "Studies on Sensitization of Animals with Simple Chemical Compounds", J. Exp. Med. 61:643-656, 1935.
- GLP compliance:
- not specified
- Type of study:
- other: Intradermal sensitization test
Test material
- Reference substance name:
- 1-nitropropane
- EC Number:
- 203-544-9
- EC Name:
- 1-nitropropane
- Cas Number:
- 108-03-2
- Molecular formula:
- C3H7NO2
- IUPAC Name:
- 1-nitropropane
- Details on test material:
- The analytical report submitted with the sample indicated that the purity of the test material was 96.12%. The test material also contained (by weight) 2.44% 2-nitropropane, 0.64% 1-nitro-2-methyl propane, 0.42% 2-nitrobutane, 0.35% 2-nitro-2-methyl propane, and 0.013% water. Other minor impurities were not listed.
Prodution lot number 9A17-7B
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- not specified
- Sex:
- male
- Details on test animals and environmental conditions:
- Body weights 250-300 g
Study design: in vivo (non-LLNA)
Inductionopen allclose all
- Route:
- intradermal
- Vehicle:
- other: saline
- Concentration / amount:
- 5% and 1% solutions of test material in saline
Challengeopen allclose all
- Route:
- intradermal
- Vehicle:
- other: saline
- Concentration / amount:
- 5% and 1% solutions of test material in saline
- No. of animals per dose:
- 10/group
- Details on study design:
- Thirty male guinea pigs (250-300 g) were divided into 3 groups of 10 each. The animals' backs and flanks were shaved free of hair. One group was intradermally injected with 0.05 ml of a 5% solution of test material in saline. The second group (positive control) was similarly injected with 0.05 ml of a 0.3% dinitro-chlorobenzene (DNCB) solution that was solubilized in a minimum volume of alcohol and made to volume with saline. The third group (negative control) was injected with 0.05 ml of saline. After 24 hours, the injected sites were scored for erythema and edema according to the method of Draize (Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics, Association of Food and Drug Officials of the United States, p.48, 1957). at 48 hours the animals were again injected with 0.1 ml of their respective solutions. The injections were repeated 2-3 times a week until 10 injections were made.
The animals were allowed to rest for 2 weeks after the last injection. On the first day of the third week (or the 43nd day after the first injection), the animals in each group were challenged intradermally with 0.1 ml of solution at a new site. Animals from the first, second and third groups were challenged with 1% test material, DNCB solution (0.03% and 0.3%), and test material and both DNCB solutions (at different sites), respectively. After 24 hours, the injected sites were depilated with "Nair". The injected sites were scored for erythema and edema 3 hours after hair removal. The sites were rescored at 48 hours.
The test material was considered a sensitizer if the number of animals exhibiting skin reactions was higher in Group 1 than in the negative control group. The positive control group was an internal control for the test. - Challenge controls:
- Yes, dinitro-chlorobenzene
- Positive control substance(s):
- yes
- Remarks:
- dinitro-chlorobenzene
Study design: in vivo (LLNA)
- Concentration:
- Not applicable
- No. of animals per dose:
- Not applicable
- Details on study design:
- Not applicable
- Statistics:
- None
Results and discussion
- Positive control results:
- Positive control animals exhibited necrosis during the entire induction period.
In vivo (non-LLNA)
Resultsopen allclose all
- Reading:
- 1st reading
- Hours after challenge:
- 27
- Group:
- test chemical
- Dose level:
- Challenged with 0. 1 ml of 1% solution of test material
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 27.0. Group: test group. Dose level: Challenged with 0. 1 ml of 1% solution of test material. No with. + reactions: 0.0. Total no. in groups: 10.0.
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- Challenged with 0.1 ml of 1% solution of test material
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: test group. Dose level: Challenged with 0.1 ml of 1% solution of test material. No with. + reactions: 0.0. Total no. in groups: 10.0.
- Reading:
- 1st reading
- Hours after challenge:
- 27
- Group:
- positive control
- Dose level:
- Challenged with 0.1 ml of 0.3 % solution of DNCB
- No. with + reactions:
- 10
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 27.0. Group: positive control. Dose level: Challenged with 0.1 ml of 0.3 % solution of DNCB. No with. + reactions: 10.0. Total no. in groups: 10.0.
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- positive control
- Dose level:
- Challenged with 0.1 ml of 0.3% solution of DNCB
- No. with + reactions:
- 10
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: positive control. Dose level: Challenged with 0.1 ml of 0.3% solution of DNCB. No with. + reactions: 10.0. Total no. in groups: 10.0.
- Reading:
- 1st reading
- Hours after challenge:
- 27
- Group:
- positive control
- Dose level:
- Challenged with 0.1 ml of 0.03% solution of DNCB
- No. with + reactions:
- 8
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 27.0. Group: positive control. Dose level: Challenged with 0.1 ml of 0.03% solution of DNCB. No with. + reactions: 8.0. Total no. in groups: 10.0.
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- positive control
- Dose level:
- Challenged with 0.1 ml of 0.03% solution of DNCB
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: positive control. Dose level: Challenged with 0.1 ml of 0.03% solution of DNCB. No with. + reactions: 0.0. Total no. in groups: 10.0.
- Reading:
- 1st reading
- Hours after challenge:
- 27
- Group:
- negative control
- Dose level:
- Challenged with 0.1 ml of 1% solution of test material
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 27.0. Group: negative control. Dose level: Challenged with 0.1 ml of 1% solution of test material. No with. + reactions: 0.0. Total no. in groups: 10.0.
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- negative control
- Dose level:
- Challenged with 0.1 ml of 1% solution of test material
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: negative control. Dose level: Challenged with 0.1 ml of 1% solution of test material. No with. + reactions: 0.0. Total no. in groups: 10.0.
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- negative control
- Dose level:
- Challenged with 0.1 ml of 0.3% solution of DNCB
- No. with + reactions:
- 10
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 24.0. Group: negative control. Dose level: Challenged with 0.1 ml of 0.3% solution of DNCB. No with. + reactions: 10.0. Total no. in groups: 10.0.
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- negative control
- Dose level:
- Challenged with 0.1 ml of 0.3% solution of DNCB
- No. with + reactions:
- 10
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: negative control. Dose level: Challenged with 0.1 ml of 0.3% solution of DNCB. No with. + reactions: 10.0. Total no. in groups: 10.0.
- Reading:
- 1st reading
- Hours after challenge:
- 27
- Group:
- negative control
- Dose level:
- Challenged with 0.1 ml of 0.03 % DNCB
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 27.0. Group: negative control. Dose level: Challenged with 0.1 ml of 0.03 % DNCB. No with. + reactions: 0.0. Total no. in groups: 10.0.
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- negative control
- Dose level:
- Challenged with 0.1 ml of 0.03% solution of DNCB
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: negative control. Dose level: Challenged with 0.1 ml of 0.03% solution of DNCB. No with. + reactions: 0.0. Total no. in groups: 10.0.
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: Not applicable
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: Not applicable
Any other information on results incl. tables
During the
induction phase, the first 3 injections of a 5% solution of test
material caused necrosis. Therefore,
the last seven injections were made with 1%. Whether
or not any reactions were observed at this concentration was not listed.
Positive
control animals exhibited necrosis during the entire induction period.
None of the animals that were induced with test material reacted after
challenge with the test material. In
the positive control group 8/10 animals challenged with 0.03% DNCB and
all animals challenged with 0.3% DNCB had skin reactions at 27 hours. At
48 hours, all positive control animals injected with 0.3% DNCB and none
of the positive controls injected with 0.03% DNCB had positive reactions.
All
negative controls challenged with 0.3% DNCB had skin reactions at 27 and
48 hours, and none of the negative controls challenged with 0.03% DNCB
had skin reactions at 27 and 48 hours.
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information
- Conclusions:
- None of the animals that were induced with test material reacted after challenge with the test material. 1-Nitropropane is considered to be not sensitizing.
- Executive summary:
None
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