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EC number: 611-591-5 | CAS number: 57903-73-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation / corrosion
- Remarks:
- in vitro
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 431 (In Vitro Skin Corrosion: Human Skin Model Test)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- [[2-[2-[2-(3-chloro-2-hydroxy-propoxy)propoxymethyl]-3-(2-prop-2-enoyloxypropoxy)-2-(2-prop-2-enoyloxypropoxymethyl)propoxy]-1-methyl-ethyl] prop-2-enoate
- EC Number:
- 611-591-5
- Cas Number:
- 57903-73-8
- Molecular formula:
- Not specified UVCB - Reaction product of 1-chloro-2,3- epoxypropane (0-9 mol) with pentaerythritol and acrylic acid.
- IUPAC Name:
- [[2-[2-[2-(3-chloro-2-hydroxy-propoxy)propoxymethyl]-3-(2-prop-2-enoyloxypropoxy)-2-(2-prop-2-enoyloxypropoxymethyl)propoxy]-1-methyl-ethyl] prop-2-enoate
- Details on test material:
- - Name of test material (as cited in study report):Pentaerythritol, olig. react. prod. with 1-chloro-2,3-epoxypropane, react. prod. with acrylid acid
- Physical state:liquid, viscous
- Analytical purity: 100% UVCB
- Lot/batch No.: Mischcharge aus G44/082/11 und G44/085/11
Constituent 1
Test animals
- Species:
- other: EpiDerm TM Skin Corrosivity Tes
- Strain:
- other: in vitro
Test system
- Vehicle:
- unchanged (no vehicle)
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): a single topical application of 50 μL (corrosion test) or 30 μL (irritation test) - Duration of treatment / exposure:
- 3 min and 1 hour(s)
- Details on study design:
- TEST SYSTEM
The EpiDermTM model consists of normal, human-derived epidermal keratinocytes which have been cultured to form a multi layered, highly differentiated model of the human epidermis. It consists of organized basal, spinous and granular layers, and a multi-layered stratum corneum containing intercellular lamellar lipid layers arranged in patterns analogous to those found in vivo. The EpiDermTM tissues (surface 0.6 cm²) are cultured on specially prepared cell culture inserts (MILLICELLs, 10 mm ∅) and commercially available as kits (EpiDerm™ 200), containing 24 tissues on shipping agarose.
Tissue model: Epi-200
Origin: MatTek In Vitro Life Science Laboratories, Bratislava, Slovakia
TEST PROCEDURE
Corrosion test:
Two tissues per exposure time (3 minutes at room temperature or 1 hour in the incubator, as a rule) and test group (test material, negative control and positive control; 12 tissues per test) were used.
Fifty microliter (50 μL) of the undiluted liquid test substance was applied using a pipette. Control tissues were concurrently applied with 50 μL of de-ionized water (negative control, NC) or with 50 μL of 8 n potassium hydroxide (positive control, PC).
The tissues were washed with PBS to remove residual test material 3 minutes or 1 hour afterstart of the application treatment. Rinsed tissues were kept in 24-well plates (holding plates) at room temperature on assay medium until all tissues per application time were dosed and rinsed. The assay medium was then replaced by MTT solution and tissues were incubated for 3 hours.
After incubation, tissues were washed with PBS and the formazan produced by the tissues was extracted with isopropanol. The optical density at a wavelength of 570 nm (OD570) of the extracts was determined spectrophotometrically. Blank values were established of 6 microtiter wells filled with isopropanol for each microtiter plate.
Irritation test:
Three tissues were treated with the test substance, the PC and NC, respectively. Thirty microliter (30 μL) of the undiluted liquid test substance was applied using a pipette.
Control tissues were concurrently applied with 30 μL of sterile PBS (negative control, NC) or with 30 μL of 5% SDS (positive control, PC). A nylon mesh was placed carefully onto the tissue surface afterwards.
The tissues were washed with sterile PBS to remove residual test material 1 hour after start of application. Rinsed tissues were blotted on sterile absorbent paper and transferred into new 6-well plates, pre-filled with 0.9 mL fresh medium. When all tissues were rinsed, the surface of each tissue was carefully dried with a sterile cotton swab.
Subsequently, the tissues were incubated in the incubator at 37°C for 24 ± 2 hours.
After 24 ± 2 hours the tissues were transferred into new 6-well plates pre-filled with 0.9 mL of fresh medium and placed into the incubator for additional 18 ± 2 hours post-incubation period.
After the post-incubation period, the assay medium was replaced by 0.3 mL MTT solution and the tissues were incubated in the incubator for 3 hours. After incubation, the tissues were washed with PBS to stop the MTT-incubation. The formazan that was metabolically produced by the tissues was extracted by incubation of the tissues in isopropanol. The optical density at a wavelength of 570 nm (OD570) of the extracts was determined spectrophotometrically. Blank values were established of 6 microtiter wells filled with isopropanol for each microtiter plate.
CONTROLS
Negative control (NC): De-ionized water (corrosion test); PBS, sterile (irritation test)
Positive control (PC): 8-n potassium hydroxide solution (Sigma-Aldrich, Munich, Germany) for the corrosion test
5% (w/v) sodium dodecyl sulfate (SDS, Sigma, Germany) in deionized water, sterile for the irritation test
Results and discussion
Any other information on results incl. tables
Corrosion test
|
|
Exposure: 3 min |
Exposure: 1h |
||||
Test substance |
|
Tissue 1 |
Tissue 2 |
mean |
Tissue 1 |
Tissue2 |
mean |
NC |
mean OD570
|
1.882 |
1.948 |
1.915 |
1.840 |
1.838 |
1.839 |
Viability [% of NC] |
98.3 |
101.7 |
100 |
100.1 |
99.9 |
100 |
|
12/0027-1 |
mean OD570
|
1.894 |
1.782 |
1.838 |
1.816 |
1.850 |
1.833 |
Viability [% of NC] |
98.9 |
93.1 |
96 |
98.7 |
100.6 |
100 |
|
PC |
mean OD570
|
0.330 |
0.376 |
0.353 |
0.139 |
0.193 |
0.166 |
Viability [% of NC] |
17.2 |
19.6 |
18 |
7.6 |
10.5 |
9 |
Irritation test
Test substance |
|
Tissue 1 |
Tissue 2 |
Tissue 3 |
Mean |
SD |
NC |
mean OD570
|
2.036 |
1.728 |
2.221 |
1.995 |
|
Viability [% of NC] |
102.0 |
86.6 |
111.3 |
100 |
12.49 |
|
12/0027-1 |
mean OD570
|
1.781 |
1.496 |
1.403 |
1.560 |
|
Viability [% of NC] |
89.3 |
75.0 |
70.3 |
78 |
9.88 |
|
PC |
mean OD570
|
0.058 |
0.063 |
0.058 |
0.060 |
|
Viability [% of NC] |
2.9 |
3.2 |
2.9 |
3 |
0.15 |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.