Registration Dossier
Registration Dossier
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EC number: 416-900-5 | CAS number: 79723-02-7 TMAP
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The toxicity of the substance to microorganisms was determined in a 3 h activated sludge respiration inhibition study carried out according to OECD guideline No. 209 and GLP principle. Under the conditions of the final test, the substance did not significantly inhibit the respiration rate of waste water at concentrations upto 1000 mg/L. Therefore the NOEC is 1000 mg/L and the EC50 is > 1000 mg/L
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
The toxicity of the substance to microorganisms was determined in a 3 h activated sludge respiration inhibition study carried out according to OECD guideline No. 209 and GLP principles.
In a combined limit/range-finding test, microorganisms in activated sludge were exposed to the test substance at a concentration of 10, 100 and 1000 mg/L under static conditions.Under test conditions the substance significantly inhibited the respiration rate of waste water (activated sludge) at 1000 mg/L. However, the EC50 exceeded the highest test concentration of 1000 mg/L and no NOEC could be determined. There was no oxygen uptake from abiotic processes and the result at 1000 mg/L with a nitrification inhibitor showed that the heterotrophic inhibition of the respiration rate was 4%. Based on the results of the total and heterotrophic respiration, nitrification inhibition was calculated to be 28%.
In the final test, microorganisms in activated sludge were exposed to the test substance at a concentration of 0, 10, 32, 100, 320 and 1000 mg/L under static conditions. No statistically significant inhibition of the respiration rate of the sludge was recorded at any of the concentrations tested.
In the combined limit/range-finding test a significant effect was observed at 1000 mg/l, based on three replicates. However, considering the higher number of replicates (five replicates) the results of the final test were used for determination of the effect parameter
All criteria for acceptability of the test were met and this study was considered to be valid. The present toxicity study is classified as reliable without restrictions according to the OECD guideline No. 209.
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