2-chlorobenzothiazole

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

other information

Study period:

1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: UBA-Verfahrensvorschlag "Letale Wirkung beim Zebrabaerbling - Brachydanio rerio" (LC0, LC50, LC100; 48-96 Stunden), May 1984

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-chlorobenzothiazole

EC Number:

210-415-0

EC Name:

2-chlorobenzothiazole

Cas Number:

615-20-3

Molecular formula:

C7H4ClNS

IUPAC Name:

2-chloro-1,3-benzothiazole

Details on test material:

purity: 99.4 % w/w

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Analytical sampling: daily

Test solutions

Vehicle:

no

Details on test solutions:

The following nominal concentrations were tested: -
- 2.8 mg/L
- 3.9 mg/L
- 5.5 mg/L
- 7.8 mg/L
- 11 mg/L

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

3-months-old fish used:
- 10 animals for each of the five test vessels for 5 concentrations
- 10 animals for the control
Length: 2.5 to 3.5 cm/animal
Fresh weight (measured 10 animals)
- 1.64 to 2.42 g (test vessels)
- 1.54 g (control)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

Test temperature was between 22.2 and 22.9 °C

pH:

pH-value was between 7.1 and 8.4

Dissolved oxygen:

Oxygen concentration varied between 8.0 and 8.7 mg/L which equals an oxygen saturation of 95.2 to 103.6 % depending on dissolved oxygen concentration and temperature

Salinity:

freshwater

Nominal and measured concentrations:

Measured. However, in all test vessels undissolved particles (oily droplets) of the substance were visible at the surface of the test medium.
The detected concentrations of the substance in the aquatic phase were below the nominal concentrations (< 80 %).

Details on test conditions:

- The test was conducted in ventilated vessels (300 x 135 x 200 mm) each filled with 5 L of test medium.
- Synthetic freshwater (in accordance with ISO, aerated) was used for dilution

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

3.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

5.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

7.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

The GDCh-Advisory Committee on Existing Chemical s (BUA) has evaluated the study and the study was found to be valid (2005, BUA Report 256 2-Chlorobenzothiazole, Stuttgart, S. Hirzel Wissenschaftliche Verlagsgesellschaft)

Conclusions:

The LC50 of the substance is 5.5 mg/L nominal in a 96 h fish test according to UBA-Verfahrensvorschlag "Letale Wirkung beim Zebrabaerbling - Brachydanio rerio" from 1984