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EC number: 200-795-6 | CAS number: 73-22-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-03-12 to 2008-04-23
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study according to OECD guideline 403 (adopted 1981)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Version / remarks:
- traditional inhalation LC50 study
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.2 (Acute Toxicity (Inhalation))
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- L-tryptophan
- EC Number:
- 200-795-6
- EC Name:
- L-tryptophan
- Cas Number:
- 73-22-3
- Molecular formula:
- C11H12N2O2
- IUPAC Name:
- L-tryptophan
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
Source: Charles River Deutschland GmbH, Sulzfeld, Germany
Age at study initiation: 8 – 9 weeks at time of administration
Body weight: 229 - 322 g before exposure
Housing: single caging in Makrolon type III cages; wire mesh lids
Diet: Ssniff R/M-H maintenance diet ad libitum
Water: tap water, ad libitum
Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
Temperature (°C): 21.7-24.3
Humidity (%): 46.2-64.9
Air changes (per hr): 12
Photoperiod (hrs dark / hrs light): 12 / 12
Administration / exposure
- Route of administration:
- inhalation: dust
- Type of inhalation exposure:
- nose only
- Vehicle:
- clean air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
Exposure apparatus: exposure chamber from TSE, Technical & Scientific Equipment GmbH, Germany
Exposure chamber volume: 19 L
System of dust generating: dust generator Technical & Scientific Equipment GmbH, Germany
Method of particle size determination: cascade impactor
Rate of air: 700 L/h
MMAD: 3.1 um - Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- Measurement by gravimetric analysis. Dust was collected 12 times during exposure period. The exact amount of collected air was measured by a gas meter.
- Duration of exposure:
- 4 h
- Concentrations:
- Target concentration: 5 mg/L
Actual concentration of the dust: 5.17 mg/L - No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- Duration of observation period following administration: 14 days
Frequency of observations: 1 - 6 hours after the start of exposure and daily thereafter
Frequency of weighing: day 0 and on days 7 and 14 following administration
Necropsy of survivors performed: yes
Other examinations performed: clinical signs, body weight - Statistics:
- Means and standard deviations of each test group were calculated for body weight and body weight gain. Median was calculated for environmental condition in the animal room. The particle size distribution was statistically evaluated based on probit analysis. As a limit test was performed, no calculation of the LC50 was possible.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 5.17 mg/L air
- Exp. duration:
- 4 h
- Mortality:
- No mortality.
- Clinical signs:
- other: All animals were normal during the whole 14-day observation period
- Body weight:
- Mean body weights: Prior to study: 322 g (males) and 229 g (females); End of study: 407 g (males) and 252 g (females)
Mean weight gain: days 0-7: 38.4 g (males) and 5.4 g (females); days 7-14: 45.8 g (males) and 18.0 g (females)
Two females lost weight in the first week after exposure - Gross pathology:
- Nothing abnormal was seen in any of the animals
Applicant's summary and conclusion
- Interpretation of results:
- practically nontoxic
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- L-tryptophan feed grade was tested in a GLP study according to OECD guideline 403. The 4h-inhalation exposure of male and female Sprague-Dawley rats at a concentration of 5.17 mg/L gave no indications for adverse effects. Therefore, the 4h-LC50 is greater than 5.17 mg/L air.
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