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EC number: 224-137-2 | CAS number: 4210-32-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
An acute oral toxicity study with 4-tert-Butylbenzonitrile according to OECD 423 is available.
The LD50 (oral) of 4-tert-Butylbenzonitrile is estimated to be higher than 200 mg and less than 500 mg per kg body weight in rats.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- january- october 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 tris (Acute Oral Toxicity - Acute Toxic Class Method)
- GLP compliance:
- yes
- Test type:
- acute toxic class method
- Limit test:
- no
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Forschungsinstitut für Versuchstierzucht, A-2325 Himberg.
- Age at study initiation: Approximately 8 weeks (males) and 12 weeks (females) at the time of administration.
- Weight at study initiation: 186 - 246 g
- Fasting period before study: Feed was withdrawn the evening before the administration of the test substance and was offered again about three hours afterwards.
- Housing: Single caging in Makrolon cage type II (39 cm x 23 cm x 15 cm). Wire mesh lids. Sanitization of cages once a week.
- Diet (e.g. ad libitum): Altromin 1314 forte, gamma irradiated with 25 kGy 60Co ad libitum. (Producer: Altromin GmbH, D-32791 Lage)
- Water (e.g. ad libitum): Tap water from an automatical watering system, ad libitum.
- Acclimation period: Between 5 and 11 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): Average of 23 °C.
- Humidity (%): Average of 46 %.
- Air changes (per hr): 12 per hour.
- Photoperiod (hrs dark / hrs light): Artificial light from 6 a.m. to 6 p.m. - Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on oral exposure:
- MAXIMUM DOSE VOLUME APPLIED: 10 mL per kg body weight.
- Doses:
- 200 mg/kg body weight (males)
200 mg/kg body weight (females)
2000 mg/kg body weight (males)
500 mg/kg body weight (males) - No. of animals per sex per dose:
- three males and three females (200 mg/kg body weight)
three females (200 mg/kg body weight)
three males (2000 mg/kg body weight)
three males (500 mg/kg body weight) - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: performed within the periods 0 - 0.5, 0.5 - 1, 1-2, 2 - 4 and 4 - 6 hours after the administration (p.a.) of the test substance and at least once a day for a total of 2 weeks. Observations included, but were not limited to changes of the skin, the fur, the eyes, the occurrence of secretions and excretions, autonomic activity, changes in gait, posture and the presence of convulsions.
- Frequency of weighing: body weights were determined before administration, 7 days p.a. and 14 days p.a.
- Necropsy of survivors performed: yes - Preliminary study:
- not appicable
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Remarks:
- oral
- Effect level:
- > 200 - < 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- 200 mg/kg body weight (males): 3/3 rats survived until the scheduled termination
200 mg/kg body weight (females): 3/3 rats survived until the scheduled termination.
2000 mg/kg body weight (males): 3/3 dies spontaneously within 20 minutes after the administration of the test substance.
500 mg/kg body weight (males): 3/3 rats died spontaneously within one day after the administration of the test substance. - Clinical signs:
- other: All animals were affected. The findings, observed between the administration and 6 h p.a., were: - Autonomous nervous effects: Piloerection in all animals, increased salivation in nearly all males. - Central nervous effects: Convulsions, sedation and hype
- Gross pathology:
- 2/3 spontaneously died males, dosed with 500 mg/kg body weight were affected. The post mortem findings were stomach ulcera and paleness of the gastic mucosa. In the other groups no abnormal findings were made.
- Other findings:
- There was no indication for a sex difference in the susceptibilty to the test substance.
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- 4-tert-Butylbenzonitrile caused effects on the autonomous and the central nervous system and sings of gerneral malaise at doses of 2000 and 500 mg/kg body weight, leading eventually to a 100 % mortality. A dose of 200 mg/kg body weight was suvived with only a few signs of reduced well-being.
The LD50 (oral) of 4-tert-Butylbenonitrile is therefore estimated to be highr than 200 mg and less than 500 mg per kg body weight in rats.
According to EC-Guideline, the test substance 4-tert-Butylbenzonitrile should be classified as harmfull if swallowed. - Executive summary:
In an acute oral toxicity study with rats (according to OECD 423 "acute toxic class method") the test substance caused effects on the autonomous and the central nervous system and signs of general malaise at the doses of 2000 and 500 mg/kg body weight, leading eventually to a 100 % mortality. A dose of 200 mg/kg body weight was survived with only a few signs of reduced well-being.
The LD50 (oral) of 4-tert-Butylbenzonitrile is estimated to be higher than 200 mg and less than 500 mg per kg body weight in rats.
According to EC-guideline, the test substance 4-tert-Butylbenzonitrile should be classified as harmfull if swallowed.
Reference
Table 2: Synopsis of the results
Dose (mg/kg) |
Sex |
Animal No. |
Number of animals |
||
exposed |
affected |
dead |
|||
200 |
m |
21 - 23 |
3 |
3 |
0 |
200 |
f |
26 - 28 |
3 |
3 |
0 |
2000 |
m |
31 - 33 |
3 |
3 |
3 |
500 |
m |
41 - 43 |
3 |
3 |
3 |
Table 3: Time of death
Dose (mg/kg) |
Sex |
Animal No. |
Time of death (p.a.) |
200 |
m |
21 - 23 |
Animals survived |
200 |
f |
26 - 28 |
Animals survived |
2000 |
m |
31 |
20 minutes |
32 |
< 20 minutes |
||
33 |
< 20 minutes |
||
500 |
m |
41 |
< 4 hours |
42 |
< 2 hours |
||
43 |
< 1 day |
Table 4: Body weights and body weight gain (Individual data, mean and standard deviation sd).
Dose (mg/kg) Sex |
Animal No. |
Body weight (g) |
Body weight gain |
||||
Before administration |
7 days p.a. |
14 days p.a. |
death |
0 - 7 days p.a. |
7 - 14 days p.a. |
||
200 m |
21 |
203 |
261 |
293 |
- |
58 |
32 |
22 |
195 |
243 |
282 |
- |
48 |
39 |
|
23 |
189 |
238 |
283 |
- |
49 |
45 |
|
mean |
196 |
247 |
286 |
- |
52 |
39 |
|
SD |
7 |
12 |
6 |
- |
6 |
7 |
|
200 f |
11 |
192 |
226 |
232 |
- |
34 |
6 |
12 |
194 |
225 |
231 |
- |
31 |
6 |
|
13 |
186 |
203 |
228 |
- |
17 |
25 |
|
mean |
191 |
218 |
230 |
- |
27 |
12 |
|
SD |
4 |
13 |
2 |
- |
9 |
11 |
|
2000 m |
31 |
239 |
a) |
a) |
- |
a) |
a) |
32 |
246 |
a) |
a) |
- |
a) |
a) |
|
33 |
239 |
a) |
a) |
- |
a) |
a) |
|
mean |
241 |
- |
- |
- |
- |
- |
|
SD |
4 |
- |
- |
- |
- |
- |
|
500 m |
41 |
208 |
a) |
a) |
- |
a) |
a) |
42 |
214 |
a) |
a) |
- |
a) |
a) |
|
43 |
216 |
a) |
a) |
- |
a) |
a) |
|
mean |
213 |
- |
- |
- |
- |
- |
|
SD |
4 |
- |
- |
- |
- |
- |
a) Animal died spontaneously
Table 5: Clinical observations. A grade of severity was recorded where applicable (low - medium - high).
Findings |
Dose (mg/kg), sex |
No. of the affected animal |
Observation time (p.a.) first last |
Maximum grade of severity |
normal at any time |
200 m |
None |
- / - |
- |
200 f |
None |
- / - |
- |
|
2000 m |
None |
- / - |
- |
|
500 m |
None |
- / - |
- |
|
complete / incomplete eyelid closure |
200 m |
21 |
1 h / 2 h |
- |
22 |
2 h / 4 h |
- |
||
23 |
2 h / 2 h |
- |
||
200 f |
26 |
4 h / 6 h |
- |
|
28 |
4 h / 4h |
- |
||
chromodacryorrhoea |
500 m |
*43 |
6 h / 6 h |
low |
piloerection |
200 m |
21 |
2 h / 6 h |
low |
22 |
2 h / 6 h |
low |
||
23 |
2 h / 6 h |
low |
||
200 f |
26 |
1 h / 6 h |
low |
|
27 |
1 h / 6 h |
low |
||
28 |
1 h / 6 h |
low |
||
500 m |
*41 |
0.5 h / 2 h |
low |
|
*42 |
0.5 h / 1 h |
low |
||
*43 |
0.5 h / 6 h |
low |
||
increased salivation |
2000 m |
*31 |
0.5 h / 0.5 h* |
medium |
*32 |
0.5 h / 0.5 h* |
medium |
||
*33 |
0.5 h / 0.5 h* |
medium |
||
500 m |
*42 |
1 h / 1 h* |
low |
|
*43 |
2 h / 6 h* |
low |
||
abnormal posture |
2000 m |
*31 |
0.5 h / 0.5 h* |
- |
|
*32 |
0.5 h / 0.5 h* |
- |
|
|
*33 |
0.5 h / 0.5 h* |
- |
|
tonic convulsions |
500 m |
*42 |
0.5 h / 1 h* |
- |
epileptiform convulsions |
2000 m |
*31 |
0.5 h / 0.5 h* |
high |
|
*32 |
0.5 h / 0.5 h* |
high |
|
|
*33 |
0.5 h / 0.5 h* |
high |
|
hyperalgesia |
2000 m |
*32 |
0.5 h / 0.5 h* |
- |
500 m |
*33 |
0.5 h / 0.5 h* |
- |
|
*42 |
1 h / 1 h* |
low |
||
sedation |
500 m |
*41 |
0.5 h / 2 h* |
low |
|
*42 |
0.5 h / 1 h* |
medium |
|
|
*43 |
0.5 h / 6 h* |
medium |
*: animal died spontaneously / finding lasted till death
Table 6: Necropsy Findings. Number examined: 9 males ans 3 females.
System Organ, finding |
Dose (mg/kg) |
Sex |
Nos. of the affected animals |
No findings |
200 |
m |
21, 22, 23 |
200 |
f |
26, 27, 28 |
|
2000 |
m |
31, 32, 33 |
|
500 |
m |
41 |
|
Alimentary System Glandular stomach, mucosa, ulcera |
500 |
m |
42 |
Glandular stomach, mucosa, pale |
500 |
m |
43 |
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
Additional information
In an acute oral toxicity study with rats (according to OECD 423 "acute toxic class method") the test substance caused effects on the autonomous and the central nervous system and signs of general malaise at the doses of 2000 and 500 mg/kg body weight, leading eventually to a 100 % mortality. A dose of 200 mg/kg body weight was survived with only a few signs of reduced well-being.
The LD50(oral) of 4-tert-Butylbenzonitrile is estimated to be higher than 200 mg and less than 500 mg per kg body weight in rats.
No data is available on the acute dermal or inhalation toxicity of 4 -tert-Butylbenzonitrile
Justification for classification or non-classification
According to the results of the test regarding acute oral toxicity (according to OECD 423 "acute toxic class method") 4 -tert-Butylbenzonitrile is classified with H302: Harmfull if swallowed.
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