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EC number: 929-513-6 | CAS number: 1400584-86-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 2019
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- sodium (5s,8s)-8-methoxy-2,4-dioxo-1,3-diazaspiro[4.5]decan-3-ide
- EC Number:
- 929-513-6
- Cas Number:
- 1400584-86-2
- Molecular formula:
- C9H13N2O3 · Na
- IUPAC Name:
- sodium (5s,8s)-8-methoxy-2,4-dioxo-1,3-diazaspiro[4.5]decan-3-ide
- Test material form:
- solid: bulk
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling schedule:
Control: at 0 and 96 hours
Test concentration: at 0 and 96 hours
Test solutions
- Vehicle:
- no
- Details on test solutions:
- To achieve the desired test concentration 502.0 mg of the test item were added to 5 litres of dilution water.
The pH was to be pH 9.2 and was adjusted to pH 7.9.
The stock solution was directly used to prepare the exposure concentration.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Name: Zebra fish
Source: Aqua KlöGer (Germany)
Date supplied: 2007-02-13
Acclimatisation: Stock held since 2007-02-13 and acclimatised to the test conditions since then.
Temperature: 20 - 24 °C
Dissolved oxygen: > 5 mg/L
Feeding: Commercial fish food, daily. Discontinued 24 h prior to test.
Mortalities during acclimatisation period: < 5 %
Medication: none
Mean standard length (n = 20): 3.5 cm (S.D. = 0.20 cm)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- The total hardness of the dilution water, measured at test start, was 15.5 °dH (= 277 mg/L CaCO3).
- Test temperature:
- 21.0 - 22.6 °C
- pH:
- 7.7 - 8.1
- Dissolved oxygen:
- 8.1 - 8.5 mg/L
- Nominal and measured concentrations:
- nominal: 100 mg/L
measured: 99.6 mg/L (0 and 96 hours) - Details on test conditions:
- Reconstituted water prepared according to the recommendations of ISO 7346. This freshly prepared standard dilution water is used for both, the maintenance of the test animals under flow-through conditions and the preparation of stock and test solutions of the test item.
Exposure conditions
Test vessels: glass aquaria holding 5 L of test media
Experimental design:
1 test concentration plus 1 control
10 animals per test concentration
Loading: 0.74 g body weight (wet weight) per litre
Photoperiod: 16 h light: 8 h dark
Aeration: gentle aeration via narrow glass tubes
Criteria of effects:
The criterion of death used in this study was the absence of response to physical stimulation.
In addition to observations on mortality at 2, 24, 48, 72 and 96 hours, subjective assessments were made on the type and incidence of sub-lethal effects compared with control fish. - Reference substance (positive control):
- not required
Results and discussion
Effect concentrations
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- No toxic effects against fish at a test concentration of 100 mg/L.
- Executive summary:
A study was performed to assess the acute toxicity of the test item to Danio rerio under static conditions.
The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (OJ. No. L383A, 29.12.92) Part C, Method 1 'Acute toxicity for Fish' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test'.
Groups of ten fish of the recommended size were exposed to a limit test concentration of nominally 100 mg/L test item dissolved in water.
Observations were made on the number of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 hours of exposure. The following values were determined:Time [h] LC 50 [mg/L] 2 > 100 24 > 100 48 > 100 72 > 100 96 > 100 No toxic effects against fish at a test concentration of 100 mg/L.
All results are expressed in terms of nominal concentrations. Recovery rates correspond to 99.6% of nominal values at 0 and 96 hours, respectively.
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