Oxirane, 2-(2-fluorophenyl)-2-(4-fluorophenyl)-

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19.06.2006 - 23.06.2006

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: OSPAR Commission 2005: Protocols on Methods for the Testing of Chemicals used in the Offshore Industry (reference number: 2005-11 [a revised version of agreement 1995-07])

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-(2-Fluorophenyl)-2-(4-fluorophenyl)oxirane

Cas Number:

88374-05-4

Molecular formula:

C14H10F2O

IUPAC Name:

2-(2-Fluorophenyl)-2-(4-fluorophenyl)oxirane

Details on test material:

- Name of test material (as cited in study report): fluorphenylepoxyethan
- Physical state: liquid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: (Water accomodated fraction (WAF)): 40, 100, 200, 400 and 1000 ml WAF/l
- Sampling method: 2 x 50 ml of stock solution
- Sample storage conditions before analysis: Freezer at -20ºC

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Cyprinodon variegatus

Details on test organisms:

TEST ORGANISM
- Common name: Sheephead minnow
- Source: originally obtained from a stock culture at Opus Plus Ltd.(the former ERT Orkney water Technology Centre, and cultured at DHI since June 2003. Animals from this DHI culture was used for this study

ACCLIMATION
- Acclimation conditions : no acclimatation needed, test done under same conditions at same facility as test animals was optained from.

Study design

Test type:

semi-static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

21±0.5ºC

pH:

7.9-8.1

Dissolved oxygen:

> 70%

Salinity:

31 ‰

Nominal and measured concentrations:

Stock solution made by 1000 mg substance dissolved in 2000 ml test media.
Nominal concentration: 500 mg/l.
Substance was not fully dissolved, therfore a test was taken from the middle of the volumetric flask (water accomodated fraction = WAF)
The stock solution was analysed to be 16.63 mg/l.

Details on test conditions:

TEST SYSTEM
- Test vessel: 2 L aquariums containing 1 L test solution
- Renewal rate of test solution (frequency/flow rate): after 48 h.
- No. of organisms per vessel: 10

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol (3,5-CDP), CAS No. : 591-35-5, at 2.3 mg/L & 4.6 mg/l

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC10

Effect conc.:

3.33 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

dissolved

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

4.26 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

dissolved

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- Mortality: 50% at 2.3 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

Mortality in controls < 10 %, oxygen > 60%, mortality with reference test within acceptable range, 20-80 %, average weight of the fish was 0.06±0.02 g giving maximal 1 gram fish per liter testsolution

Conclusions:

LC50(96 h) of fluorphenylepoxyethan, CAS No. 88374-05-4 is 4.26 mg/L for Cyprinodon variegatus