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EC number: 218-145-5 | CAS number: 2052-25-7
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
No toxic effects against fish were observed at a limit test concentration of 100 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
A study was performed to assess the acute toxicity of Mordant Black 9 to Danio rerio under semi static conditions.
The study was conducted in accordance with Council Regulation (EC) No 440/2008, Method C.1 'Acute toxicity for Fish' (2008) which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992).
A group of seven fish of the recommended size was exposed to a limit test concentration of nominally 100 mg/L of Mordant Black 9 dissolved in dilution water. Auxiliaries used to prepare the test media were an ultrasonic bath and a magnetic stirrer.
Observations were made on the number of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 hours of exposure. The following values were determined:
Time LC 50
[h] [mg/L]
2 > 100
24 > 100
48 > 100
72 > 100
96 > 100
No toxic effects against fish were observed at a limit test concentration of 100 mg/L.
All results are expressed in terms of nominal concentrations. Effective concentrations ranged from 102.1 % to 104.4 % of nominal values in the freshly prepared media and from 100.0 % to 101.2 % of nominal values in the media after 24 hours of exposure.
The hardness of the dilution water used was 13.6 °dH (= 243 mg/L CaCO3).
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