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EC number: 219-340-8 | CAS number: 2420-17-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- end on XXX
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: study performed according to OECD guideline and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 5-(4-hydroxyphenyl)imidazolidine-2,4-dione
- EC Number:
- 219-340-8
- EC Name:
- 5-(4-hydroxyphenyl)imidazolidine-2,4-dione
- Cas Number:
- 2420-17-9
- Molecular formula:
- C9H8N2O3
- IUPAC Name:
- 5-(4-hydroxyphenyl)imidazolidine-2,4-dione
- Details on test material:
- - Name of test material (as cited in study report): p-Hydroxy-phenylhydantoin
- Substance type: monoconstituent substance
- Physical state: powder
- Stability under test conditions: will be tested within this study (see analytical section)
- Storage condition of test material: at room temperature, protected from light
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 1, 2, 4, 8 and 10 mg/L
- Sampling method: data not available
- Sample storage conditions before analysis: concentrations were measured immediately after sampling, as it was determined that the test item was not stable upon freezing and thawing procedure originally performed with the test treatments – the test item disappeared during storage at -18°C.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
The test item was introduced into the test solution in one step.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain: Daphnia magna STRAUS (clone 5)
- Source: laboratory bred
- Age at study initiation: not older than 24h
- Method of breeding: data not available
- Feeding during test: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- data not available
- Test temperature:
- 20.7 to 21.6°C
- pH:
- t0: 9.18 (control) and 8.85 (10 mg/L)
t48: 9.13 (control) and 8.56 (10 mg/L) - Dissolved oxygen:
- between 5.2 and 8.7 mg O2/L
- Salinity:
- not applicable (freashwater)
- Nominal and measured concentrations:
- nominal: 0, 1, 2, 4, 8, 10 mg/L
measured at t0 : 0, 0.950, 2.230, 4.480, 7.840, 9.080 mg/L
measured at t48: 0, 0.820, 0.060, 0.530, 1.230, 1.670 mg/L
geom. mean of t0 vs. t48h: 0.88, 0.37, 1.54, 3.11, 3.89 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL carboys with glass stoppers made of glass
- Type: closed (stoppers made of glass were used in order to minimize losses due to evaporation)
- Aeration: test vessel was gently agitated
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
The stock solutions were individually prepared in ultrapure water.
Medium: dilution water according to Elendt M4.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h of light and 8h of darkness
- Light intensity: data not available
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The effect of the test item to the animals was examined by the determination of the swimming behaviour of the daphnia. For this purpose the respective test vessel was gently agitated. Daphnia showing no swimming within 15 sec. were considered as immobile. Moving of antenna was not considered as swimming.
The effect was determined at 24 and 48h.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: between 1.25 and 2
- Range finding study
- Test concentrations: an "enlarged screening-test" was performed in order to fix the concentration range of the test item for the main test, because the results of the first screening test were not clear enough. This enlarged screening was prepared with following concentrations of the test item: 1.0, 5.0, and 10.0 mg, respectively.
- Results used to determine the conditions for the definitive study: 100% mortality was observed at 5 and 10 mg/L after 48h (0% at 1 mg/L) - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (prior to beginning of the test)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.43 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- swimming behaviour
- Remarks on result:
- other: no limits given
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.48 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- swimming behaviour
- Remarks on result:
- other: no limits given
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.88 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- swimming behaviour
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.37 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- swimming behaviour
- Details on results:
- Under the conditions used for the test, immobilisa¬tion of the daphnia was observed at nominal concentrations beginning with 2 mg/L.
As a conclusion of the analytical part of this study, it can be concluded that the test item was not stable upon freezing and thawing procedures and not stable during incubation in the test. Recoveries of the concentrations determined after 48h were in the range of 3.0 to 82.0 % of those determined at time t0. Based on this, the reported effect concentrations refer to mean (geometric mean) of the measured concentrations. The test is considered valid as all criterions for validity were met. - Results with reference substance (positive control):
- The value of the EC50 (24h) was 1.06 mg/L potassium dichromate.
This value is in accordance with the recommendations given in OECD Guideline 202. - Reported statistics and error estimates:
- The statistical calculations were done according to the Guideline using the commercial computer program ToxRatPro Version 2.10.
Any other information on results incl. tables
Table 1: result (Main test)
Nominal Concentration of the testitem(mg/L) |
Ratio immobilised Daphnia [%] |
pH-Value |
O2-Content [mg O2/L] |
Temperature [°C] |
|||||
24h |
48h |
t0 |
48h |
t0 |
48h |
t0 |
48h |
||
Control (0) |
0 [0/20] |
0 [0/20] |
9.18 |
9.13 |
8.7 |
7.9 |
20.8 |
21.4 |
|
1 |
|
0 [0/20] |
5 [1/20] |
9.12 |
9.02 |
6.6 |
6.9 |
20.7 |
21.3 |
2 |
|
0 [0/20] |
75 [15/20] |
9.10 |
8.97 |
6.3 |
6.8 |
20.7 |
21.3 |
4 |
|
80 [16/20] |
100 [20/20] |
9.07 |
8.95 |
6.2 |
6.3 |
20.8 |
21.4 |
8 |
|
100 [20/20] |
100 [20/20] |
8.93 |
8.70 |
6.9 |
5.8 |
21.6 |
21.6 |
10 |
|
100 [20/20] |
100 [20/20] |
8.85 |
8.56 |
6.9 |
5.2 |
21.6 |
21.6 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- p-Hydroxy-phenylhydantoin:
- 48h NOEC = 0.88 mg/L
- 48h EC50 = 0.48 mg/L
(mean measured concentrations) - Executive summary:
p-Hydroxy-phenylhydantoin was tested for acute toxicity towards Daphnia according to OECD-Test Guideline 202 (GLP). In order to investigate the influence of the test item towards the daphnia the swimming behaviour of the animals was recorded. Under the conditions used for the test, immobilisation of the daphnia was observed at nominal concentrations beginning with 2 mg/L. The following EC-values were calculated on basis of the nominal concentrations being tested:
EC-Values
Test Results on the Basis of Nominal Concentrations
t24h
t48h
NOEC
2.00 mg/L
1.00 mg/L
LOEC
4.00 mg/L
2.00 mg/L
EC10
3.11 mg/L [no limits given]
1.12 mg/L [0.77 – 1.34]
EC20
3.28 mg/L [no limits given]
1.27 mg/L [0.96 – 1.49]
EC50
3.62 mg/L [no limits given]
1.63 mg/L [1.38 – 1.92]
EC-Values
Test Results on the Basis of Mean Measured Concentrations (geometric mean)
t24h
t48h
NOEC
0.37 mg/L
0.88 mg/L
LOEC
1.54 mg/L
0.37 mg/L
EC10
1.27 mg/L [no limits given]
n.d.
EC20
1.32 mg/L [no limits given]
n.d.
EC50
1.43 mg/L [no limits given]
0.48 mg/L [no limits given]
Values in brackets indicate the 95% confidence limits. As a conclusion of the analytical part of this study, it can be concluded that the test item was not stable upon freezing and thawing procedures and not stable during incubation in the test. Recoveries of the concentrations determined after 48h were in the range of 3.0 to 82.0 % of those determined at time t0. Based on this, the reported effect concentrations refer to mean (geometric mean) of the measured concentrations.The test is considered valid as all criterions for validity were met.
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