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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_environmental-fate-and-pathways.png)
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Details on test material:
- - Name of test material (as cited in study report): WS400517
- Physical state: Off-white to pale yellow powder
- Analytical purity: Not applicable (complex mixture)
- Lot/batch No.: HH-09-168
- Expiration date of the lot/batch: 21 January 2011
- Storage condition of test material: Room temperature in the dark
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Worlingworth sewage treatment works (Suffolk, UK)
- Method of cultivation / Preparation of inoculum for exposure: At the time of collection, the sludge was sieved (1 mm²) then transported to the laboratory and left stand for approximately 30 minutes to allow the sewage solids to settle. A portion of the supernatant was removed and the sludge aerated until required. - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 76 mg/L
- Based on:
- COD
- Initial conc.:
- 13.8 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral salts medium (MSM) according to guideline, prepared using tap-water that had been softened and treated by reverse osmosis and then purified; nominal resistivity ≥ 18 MegOhm.cm. The pH wasadjusted to 7.4 ± 0.2 with 5M HCl. This water complies with the relevant standards (British Standard (BS) 3978, 1987; American Society for Testing and Materials (ASTM) D 1193-06) for classification as Grade 1 or Type 1 water for laboratory use.
- Test temperature: 20 to 22°C.
- pH: 7.5 at the start of the test and 7.4 to 7.6 at the end
- pH adjusted: not necessary
- Suspended solids concentration: 30 mg/L
- Continuous darkness: not reported
- Other: The inoculum was added to the culture bottles 1 day before test initiation to allow a period of ageing.
TEST SYSTEM
- Culturing apparatus: glass culture bottles in a temperature controlled water bath
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: internal oxygen generating process
- Measuring equipment: Co-ordinated Environmental Services (CES) Ltd automated respirometer
SAMPLING
- Sampling frequency: continuous monitoring of the cumulative amount of oxygen consumed; the cumulative oxygen demand made by each cell was printed at, typically, hourly intervals
CONTROL AND BLANK SYSTEM
- Inoculum blank (2 flasks): inoculated mineral salts medium (MSM)
- Reference (1 flask): inoculated MSM + sodium benzoate (50 mg O₂/L)
- Toxicity control (1 flask): inoculated MSM + test material (76 mg O₂/L) + sodium benzoate (50 mg O₂/L)
- Nitrification test (1 flask): inoculated MSM + test material (76 mg O₂/L) + Allylthiourea (ATU, 11.6 mg/L)
- Nitrification control (1 flask): inoculated MSM + ATU (11.6 mg/L)
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 5
- Sampling time:
- 28 d
- Details on results:
- In the nitrification controls the degradation of the test material was similar to that in uninhibited cultures (Table 1)
In the presence of test material the degradation of sodium benzoate achieved 61% after 3 days indicating that the test substance was not inhibitory to the microbial inoculum (Table 1).
BOD5 / COD results
- Results with reference substance:
- The reference substance sodium benzoate had achieved 60% of the ThOD after 4 days of incubation and 73% by Day 28 (Table 1).
Any other information on results incl. tables
Table 1: Biodegradation of the test and reference substances
% Biodegradation |
||||||
Day |
Test material |
Test material in the presence of ATU |
Sodium Benzoate (Reference) |
Sodium Benzoate in the inhibition mixture |
||
Test 1 |
Test 2 |
Mean |
||||
1 |
0 |
0 |
0 |
0 |
34 |
38 |
2 |
0 |
0 |
0 |
0 |
47 |
53 |
3 |
0 |
0 |
0 |
0 |
54 |
61 |
4 |
0 |
0 |
0 |
0 |
60 |
- |
5 |
1 |
0 |
0 |
0 |
63 |
- |
6 |
1 |
0 |
0 |
0 |
65 |
- |
7 |
1 |
0 |
1 |
0 |
67 |
- |
8 |
1 |
0 |
1 |
0 |
68 |
- |
9 |
2 |
0 |
1 |
0 |
68 |
- |
10 |
2 |
0 |
1 |
1 |
69 |
- |
11 |
2 |
0 |
1 |
1 |
70 |
- |
12 |
3 |
1 |
2 |
1 |
70 |
- |
13 |
3 |
1 |
2 |
1 |
71 |
- |
14 |
3 |
1 |
2 |
1 |
71 |
- |
15 |
4 |
1 |
2 |
1 |
71 |
- |
16 |
4 |
2 |
3 |
1 |
71 |
- |
17 |
4 |
2 |
3 |
1 |
71 |
- |
18 |
5 |
2 |
3 |
1 |
71 |
- |
19 |
5 |
2 |
4 |
1 |
72 |
- |
20 |
5 |
2 |
4 |
1 |
72 |
- |
21 |
5 |
3 |
4 |
1 |
72 |
- |
22 |
6 |
3 |
4 |
1 |
72 |
- |
23 |
6 |
3 |
5 |
1 |
72 |
- |
24 |
6 |
3 |
5 |
2 |
73 |
- |
25 |
7 |
4 |
5 |
2 |
73 |
- |
26 |
7 |
4 |
5 |
2 |
73 |
- |
27 |
7 |
4 |
5 |
1 |
73 |
- |
28 |
7 |
4 |
5 |
1 |
73 |
- |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- the removal of the test substance in replicate values differed less than 20%, the reference compound reached the pass level by day 4, and the cumulative amount of oxygen consumed by the control mixtures was 28-37 mg O2/L
- Interpretation of results:
- under test conditions no biodegradation observed
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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