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EC number: 680-798-0 | CAS number: 886577-76-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06 - 09 Jun 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- 2011
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Staatliches Gewerbeaufsichtsamt Hildesheim
Test material
- Reference substance name:
- 2-isocyanato-2-methyl-3-(prop-2-enoyloxy)propyl prop-2-enoate
- Cas Number:
- 886577-76-0
- Molecular formula:
- C11H13NO5
- IUPAC Name:
- 2-isocyanato-2-methyl-3-(prop-2-enoyloxy)propyl prop-2-enoate
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- TOC
- Details on sampling:
- - Concentrations: All test item concentrations and the control - Sampling method: according to DIN EN 1484 at the start and the end of the exposure
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION - Method: 20 mg test item/L were freshly prepared with dilution water. This stock solution was used to achieve the required test item concentrations.- Evidence of undissolved material: stock solution was agitated until the solution was visually clear
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM - Common name: green algae - Strain: Pseudokirchneriella subcapitata SAG 61.81 - Source: Sammlung von Algenkulturen (SAG) Pflanzenphysiologisches lnstitut der Universitat Gottingen Nikolausberger Weg 18, D-37073 Gottingen, Germany - Age of inoculum: A three days old preculture, was used as inoculum - Method of cultivation: Fresh stocks are prepared every month on Z-Agar. Light intensity amounted to 35-70 μE m-2 s-1 for 24 h per day
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 0.24 mmol Ca+mg/L
- Test temperature:
- 22.0 - 23.0 °C (mean 22.5 °C)
- pH:
- 7.93 - 8.95 (control)7.73 - 8.95 (test item concentration)
- Nominal and measured concentrations:
- Control, 0.200, 0.632, 2.00, 6.32 and 20.0 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEMTest vessel: - Type: open - Material, size, headspace, fill volume: Sterile Erlenmeyer flasks (vol. 250 ml) with cotton wool plugs, 100 mL test volume - Aeration: Test containers were placed on a rotary shaker and oscillated at approximately 70 rpm - Initial cells density: approximately 5 x 10 E+03 - 10^4 cells/mL (corresponds to 6305 cells/mL) - Control end cells density: 1375608 cells/mL - No. of vessels per concentration (replicates): 3 - No. of vessels per control (replicates): 6 GROWTH MEDIUM - Standard medium used: yes TEST MEDIUM / WATER PARAMETERS - Source/preparation of dilution water: according to OECD guideline - Culture medium different from test medium: no - Intervals of water quality measurement: pH was measured at test start and test end, Temperature was measured continously and light intensity was measured prior to test start OTHER TEST CONDITIONS - Sterile test conditions: yes - Photoperiod: 24 hours/day light - Light intensity and quality: approx 4440 to 8880 lux, corresponding to 60 to 120 μE*m-2*s·-1, fluorescent tubes, OSRAM L 36 W/865, cool daylight, within ± 15 % over incubation area - Incubation: The flasks were positioned randomly and repositioned daily EFFECT PARAMETERS MEASURED: cell density via fluorescence - Determination of cell concentrations: Fluorometer: Microplate Reader Chameleon V (HIDEX) with Software Micro Win 4.41 (MIKROTEC LABORSYSTEME GMBH) - Chlorophyll measurement: measured daily via Chlorophyll a fluorescence, excitation at 436 nm, emission at 685 nm TEST CONCENTRATIONS - Spacing factor for test concentrations: dilution factor of √10Range finding study - Test concentrations: Control, 1.00, 10.0 and 100 mg/L - Results used to determine the conditions for the definitive study: The concentration range of the main test was based on the results of the range-finding test
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.47 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% Confidence Intervals
- Remarks:
- 6.34 - 6.60 mg/L
- Details on results:
- - Exponential growth in the control (for algal test): yes - Observation of abnormalities (for algal test): Microscopic evaluation of the cells at the start and end of exposure revealed no morphological abnormalities.
- Results with reference substance (positive control):
- - ErC50: 0.564 mg/L
Any other information on results incl. tables
Table 1: Biological Results: NOEC, LOEC, EC-values and 95% Confidence Intervals of the test item (0 - 72 hours) based on nominal test item concentrations [mg/L]
| Growth Rate Inhibition Nominal test item concentration [mg/L] |
NOEC | 0.632 |
LOEC | 2.00 |
ErC10 | 2.82 (2.59 - 3.06) |
ErC20 | 3.82 (3.61 - 4.02) |
ErC50 | 6.47 (6.34 - 6.60) |
| Yield Inhibition Nominal test item concentration [mg/L] |
NOEC | 0.632 |
LOEC | 2.00 |
EyC10 | 1.65 (1.38 - 1.91) |
EyC20 | 2.01 (1.78 - 2.29) |
EyC50 | 2.99 (2.63 - 3.58) |
Table 2: Analytical Results: TOC Content of the Test Media at the Start and the End of the Exposure
Nominal Test Item Concentration [mg/L] | TOC [mg C/L] | |
Test start (without algae), 0 hours | Test end (without algae), 72 hours | |
20.0 | 11.6 | 12.3 |
6.32 | 2.54 | 5.45 |
2.00 | < 2.00 | 2.63 |
0.632 | < 2.00 | < 2.00 |
0.200 | < 2.00 | < 2.00 |
Control | < 2.00 | < 2.00 |
Limit of quantification = 2.00 mg C/L
Table 3: EC50-Values of the Reference Item based on nominal concentrations [mg/L], (0-72 hours)
| Current Study | Valid Range (average ± 3 x SD) |
| Growth Rate inhibition | |
ErC50 | 0.564 | 0.797 ± 0.428 |
95 % confidence interval | 0.546 - 0.582 | |
| Yield inhibition | |
EyC50 | 0.322 | 0.415 ± 0.275 |
95 % confidence interval | 0.239 - 0.342 |
SD = Standard deviation
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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