Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 700-578-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The reliability is rated 2 because the study followed a standard guideline of reference (NF EN ISO 8692, 2005), which describes a procedure designed to evaluate this endpoint. The results were reviewed for reliability and assessed as valid. However the study was not conducted under GLP conditions.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)
- GLP compliance:
- no
Test material
- Reference substance name:
- Mono and bis and tris{tris[4-(mono and dimethylamino)phenyl]methylium} [12,21,30,32-tetrahydro-29H,31Hphthalocyanine- mono, bis and trisulfonato-k4N29,N30,N31,N32]cuprate.
- IUPAC Name:
- Mono and bis and tris{tris[4-(mono and dimethylamino)phenyl]methylium} [12,21,30,32-tetrahydro-29H,31Hphthalocyanine- mono, bis and trisulfonato-k4N29,N30,N31,N32]cuprate.
- Details on test material:
- - Name of test material (as cited in study report): Sepisol Fast Violet 2B
- Lot/batch No.: 423393
- Substance type: organocopper
- Physical state and appearance: violet dark powder
- Stability under test conditions: stable
- Storage condition of test material: at ambient temperature
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Water solubility (under test conditions): 2 mg/L with a correspondig pH of 2.8
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Age of inoculum (at test initiation): 3 days
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22°C +/2
- pH:
- T0: 8.1 - 8.2
T72: 9.0 - 9.3 - Nominal and measured concentrations:
- Nominal concentration:
0 - 1.0 - 1.2 - 1.4 - 1.6 - 1.8 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer
- Material, size, headspace, fill volume: Erlenmeyer with 60 mL of the tested solution
- Initial cells density: 10000 cells/mL
- No. of vessels per concentration : 3
- No. of vessels per control (replicates): 6
OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Light intensity and quality:4500 lux
- stirring: rev/250 min-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Mallassez counting chamber
TEST CONCENTRATIONS
- the maximum tested concentration was 1,8 mg/L. According to the maximum water solubility (stated by the supplier: 2 mg/L) and the
quantification limit of the algal growth inhibition test: 90 % of the stock solution, the maximal tested concentration was 1.8 mg/L.
A stock solution at 2 mg/L was prepared with pure water. Then a feeding media was added so as to represent 10%. of the final solution.
Therefore the highest concentration was 1.8 mg/L (0.9 x 2) - Reference substance (positive control):
- yes
- Remarks:
- K2Cr2O7
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI: 1.3 - 1.5
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 1.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI: 1.65 - 1.74
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI : 2.2 - 2.5 this is an extrapolated value
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- - Results with reference substance valid: Yes
- EC50 (72h): 1.21 mg/L (belongs to the recommended range of values: 0.92 - 1.46 mg/L)
- Other:K2CrO7 - Reported statistics and error estimates:
- CE x % calculated using the Hill equation (macro Regtox_ev6.6.2xls)
NOEC calculated with the T-test of Bonferroni (Toxcalc software)
Any other information on results incl. tables
Results at t = 24h, 48h and 72h:
t = 72 h |
||||||||
Concentration (mg/L) |
Cells Concentration x 106 |
|
|
|||||
1 |
2 |
3 |
4 |
5 |
6 |
Average Cells number |
Cells Inhibition |
|
0 |
3.65 |
3.63 |
3.82 |
3.83 |
3.57 |
4.17 |
3.78 |
|
1.0 |
3.45 |
3.68 |
4.02 |
|
|
|
3.72 |
2% |
1.2 |
3.14 |
2.40 |
2.77 |
|
|
|
2.77 |
27% |
1.4 |
2.30 |
2.18 |
1.68 |
|
|
|
2.05 |
46% |
1.6 |
1.74 |
1.46 |
1.43 |
|
|
|
1.54 |
59% |
1.8 |
1.03 |
0.88 |
0.77 |
0.89 |
76% |
t = 72 h |
||||||||
Concentration (mg/L) |
Growth Rate |
|
|
|||||
1 |
2 |
3 |
4 |
5 |
6 |
Average Cells number |
Growth rate Inhibition |
|
0 |
1.97 |
1.96 |
1.98 |
1.98 |
1.96 |
2.01 |
1.98 |
|
1.0 |
1.95 |
1.97 |
2.00 |
|
|
|
1.97 |
0% |
1.2 |
1.92 |
1.83 |
1.88 |
|
|
|
1.87 |
5% |
1.4 |
1.81 |
1.79 |
1.71 |
|
|
|
1.77 |
10% |
1.6 |
1.72 |
1.66 |
1.65 |
|
|
|
1.68 |
15% |
1.8 |
1.54 |
1.49 |
1.45 |
1.49 |
24% |
t = 48 h |
||||||||
Concentration (mg/L) |
Cells Concentration x 106 |
|
|
|||||
1 |
2 |
3 |
4 |
5 |
6 |
Average Cells number |
Cells Inhibition |
|
0 |
0.57 |
0.60 |
0.65 |
0.57 |
0.52 |
0.59 |
0.58 |
|
1.0 |
0.49 |
0.60 |
0.56 |
|
|
|
0.55 |
6% |
1.2 |
0.53 |
0.53 |
0.55 |
|
|
|
0.54 |
8% |
1.4 |
0.58 |
0.60 |
0.59 |
|
|
|
0.59 |
-1% |
1.6 |
0.409 |
0.416 |
0.425 |
|
|
|
0.42 |
29% |
1.8 |
0.34 |
0.35 |
0.39 |
0.36 |
38% |
t = 48 h |
||||||||
Concentration (mg/L) |
Growth Rate |
|
|
|||||
1 |
2 |
3 |
4 |
5 |
6 |
Average Cells number |
Growth rate Inhibition |
|
0 |
2.03 |
2.05 |
2.08 |
2.03 |
1.98 |
2.04 |
2.03 |
|
1.0 |
1.94 |
2.05 |
2.02 |
|
|
|
2.00 |
2% |
1.2 |
1.99 |
1.99 |
2.00 |
|
|
|
1.99 |
2% |
1.4 |
2.03 |
2.05 |
2.04 |
|
|
|
2.04 |
0% |
1.6 |
1.86 |
1.86 |
1.87 |
|
|
|
1.86 |
8% |
1.8 |
1.76 |
1.78 |
1.83 |
1.79 |
12% |
t = 24 h |
||||||||
Concentration (mg/L) |
Cells Concentration x 106 |
|
|
|||||
1 |
2 |
3 |
4 |
5 |
6 |
Average Cells number |
Cells Inhibition |
|
0 |
0.082 |
0.063 |
0.087 |
0.078 |
0.064 |
0.07 |
0.07 |
|
1.0 |
0.057 |
0.054 |
0.053 |
|
|
|
0.054 |
27% |
1.2 |
0.058 |
0.051 |
0.046 |
|
|
|
0.052 |
30% |
1.4 |
0.055 |
0.045 |
0.042 |
|
|
|
0.047 |
36% |
1.6 |
0.049 |
0.05 |
0.039 |
|
|
|
0.046 |
38% |
1.8 |
0.036 |
0.035 |
0.031 |
0.034 |
54% |
t = 24 h |
||||||||
Concentration (mg/L) |
Growth Rate |
|
|
|||||
1 |
2 |
3 |
4 |
5 |
6 |
Average Cells number |
Growth rate Inhibition |
|
0 |
2.11 |
1.83 |
2.17 |
2.06 |
1.86 |
1.95 |
2.00 |
|
1.0 |
1.74 |
1.68 |
1.66 |
|
|
|
1.69 |
15% |
1.2 |
1.75 |
1.64 |
1.53 |
|
|
|
1.64 |
18% |
1.4 |
1.70 |
1.51 |
1.44 |
|
|
|
1.55 |
22% |
1.6 |
1.58 |
1.61 |
1.35 |
|
|
|
1.51 |
24% |
1.8 |
1.29 |
1.26 |
1.15 |
1.23 |
38% |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the experimental conditions:
ECr 10 - 72h = 1.4 mg/L (1.3 - 1.5)
ECr 20 - 72h = 1.7 mg/L (1.65 - 1.75)
ECr 50 - 72h = 2.3 mg/L (2.2 - 2.5) which is an extrapolated value
NOEC - 72 h = 1.0 mg/L - Executive summary:
The acute algal toxicity test for the substance Sepisol Fast Violet 2B was carried out to determine the ecotoxicological profile of the test substance against an unicellular green algae. As test organism Pseudokirchnerella subcapitata was used and the NF EN ISO 8692 (2005) standard method was followed.
The parameters which were to be determined were the effective concentrations (EC) and the no observed effect concentration 72h (NOEC-72h) values with regard to the growth rate (ErC).
The following nominal concentrations were tested: 0 - 1.0 - 1.2 - 1.4 - 1.6 - 1.8 mg/L. The maximum tested concentration was 1.8 mg/L. According to the maximum water solubility (stated by the supplier as 2 mg/L) and the quantification limit of the algal growth inhibition test: 90 % of the stock solution, the maximal tested concentration was determined to be 1.8 mg/L.
A stock solution was prepared by diluting 2 mg of test material in 1 liter of pure water.
Each concentration and the controls were inoculated with approximately 10 000 algae per mL.
The test was carried out over 72 hours at a temperature of 22°C +/- 2 and constantly stirred (250 rev.min-1). Each vessel concentration was tested 3 times. 6 controls were run with the same experimental conditions. Algal concentration was counted using a Mallassez counting chamber. Once a day the extinction of an aliquot of the test-vessels (incubation) was measured.
Validation experiments and the precultivation ensured that the algal were of appropriate vialibilty and susceptibility.
The results of this study showed, that the substance Sepisol Fast Violet 2B inhibit cells and reduce the growth rate of the test organism at the tested concentrations. Under experimental conditions, the following parameters were determined:
ErC 10 - 72h = 1.4 mg/L (1.3 - 1.5)
ErC 20 - 72h = 1.7 mg/L (1.65 - 1.75)
ErC 50 - 72h = 2.3 mg/L (2.2 - 2.5) which is an extrapolated value
NOEC - 72 h = 1.0 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.