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EC number: 938-980-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 06 - July 04, 2013
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Glycine max. (L.) Merr. (Fabacee) aqueous alcoholic ext., concentrate by sequential extractions and filtration
- EC Number:
- 938-980-5
- IUPAC Name:
- Glycine max. (L.) Merr. (Fabacee) aqueous alcoholic ext., concentrate by sequential extractions and filtration
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- Test item identification : Soybean (Glycine max) dry purified extract
Batch number : 30432/M1
Internal number : 2912125-001
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- The study was performed using aerobic activated sludge from “BRIANZACQUE” civil wastewater treatment plant. Immediately after the arrival of the sludge inoculum in the laboratory, it was put under constant aeration, in dark condition at about 20 °C ± 2.0 °C. This controlled condition were kept for 2 day. Before the start of the test the activated sludge inoculum was filtered and diluted in culture medium to get a final concentration of 4 mL/L.
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 5 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- The tested concentration of test item was of 5.0 mg/L.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 48.6
- Sampling time:
- 7 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 60.1
- Sampling time:
- 14 d
- Remarks on result:
- other: Corrected values due to nitrification (after 14 the value of BOD without nitrification was 1.01 while the biodegradation value without nitrification was 64.4%)
- Parameter:
- % degradation (O2 consumption)
- Value:
- 67.4
- Sampling time:
- 28 d
- Remarks on result:
- other: ***Corrected values due to nitrification (after 28 the value of BOD without nitrification was 1.12 while the biodegradation value without nitrification was 72.0%)
- Parameter:
- % degradation (O2 consumption)
- Value:
- 70
- Sampling time:
- 21 d
- Details on results:
- After 14 days the oxygen amount used in oxidising ammonium process was determined to be equal to 0.34 mg O2/L for nitrate formation while at the test end was equal to 0.36 mg O2/L for nitrite formation. Therefore, the biodegradation value obtained after 14 and 28 days of exposure (respectively
64.4% and 72.0%, mean value between two test item replicates) was corrected considering
the oxygen uptake due to nitrification.
The obtained biodegradation value after 14 days and 28 days of incubation was calculated to be equal respectively to 60.1% and 67.4% (mean value between two test item replicates).
BOD5 / COD results
BOD5 / COD
- Parameter:
- COD
- Value:
- 1.56 mg O2/g test mat.
- Results with reference substance:
- COD = 1.67 mg O2/mg
The reference item reached the biodegradation pass level (60%) after 14 days showing that the test conditions and the inoculum were appropriate for the use in the study.
Moreover, the test item did not show any inhibitory effect on the microorganism activity at the tested concentration as demonstrated by reaching the 25% of biodegradation in the toxicity control at the 7th day of test period.
Any other information on results incl. tables
The 14-day window started after 7 days of incubation with 48.6% of biodegradation and finished at 21th day; during this period the test item showed a maximum biodegradation of 70.0% (mean percentage values between two test item replicates).
According to OECD Guideline No. 301, nitrite and nitrate produced amount at the test start
and after 7, 14, 21, 28 days of exposure was measured and the oxygen consumed amount
was calculated (Table 4, section “Evaluations”). The nitrification process occurred only at 14th
and 28th day of exposure.
After 14 days the oxygen amount used in oxidising ammonium process was determined to be
equal to 0.34 mg O2/L for nitrate formation while at the test end was equal to 0.36 mg O2/L for
nitrite formation (Table 4, section “Evaluations”).
Therefore, the biodegradation value obtained after 14 and 28 days of exposure (respectively
64.4% and 72.0%, mean value between two test item replicates) was corrected considering
the oxygen uptake due to nitrification.
The obtained biodegradation value after 14 days and 28 days of incubation was calculated to
be equal respectively to 60.1% and 67.4% (mean value between two test item replicates).
The test item, Soybean (Glycine max) dry purified extract, was found to be ready
biodegradable, having exceeded the pass level (60%) within the 14-days window (70.0%,
mean percentage value between two test item replicates).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item Soybean (Glycine max) dry purified extract has showed to be ready biodegradable under the conditions applied in a Closed Bottle method, having reached the pass level (60% biodegradation) within the 14-day window (70.0% of biodegradation reached after 21 days of incubation).
However, the test item showed no inhibitory effects on the microorganism activity at the tested concentration, as demonstrated by the 25% of biodegradation exceeded in the toxicity control after 7 days from the test start (32.3 % of biodegradation)
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