Reaction mass of 1-chloro-3-[(Z)-octadec-9-en-1-yloxy]propan-2-ol and alpha-(Z)-octadec-9-en-1-yl-omega-hydroxy-di[oxy[(chloromethyl)-1,2-ethanediyl]] and alpha-(Z)-octadec-9-en-1-yl-omega-hydroxy-tri[oxy[(chloromethyl)-1,2-ethanediyl]]

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2013-03-20

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study performed according to OECD guideline but the study was not conducted under GLP conditions.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Deviations:

no

Principles of method if other than guideline:

Not relevant

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

reaction mass of 1-chloro-3-[(Z)-octadec-9-en-1-yloxy]propan-2-ol and alpha-(Z)-octadec-9-en-1-yl-omega-hydroxy-di[oxy[(chloromethyl)-1,2-ethanediyl]] and alpha-(Z)-octadec-9-en-1-yl-omega-hydroxy-tri[oxy[(chloromethyl)-1,2-ethanediyl]]

Cas Number:

108726-95-0

Molecular formula:

C21H41O2Cl C24H46O3Cl2 C27H51O4Cl3

IUPAC Name:

reaction mass of 1-chloro-3-[(Z)-octadec-9-en-1-yloxy]propan-2-ol and alpha-(Z)-octadec-9-en-1-yl-omega-hydroxy-di[oxy[(chloromethyl)-1,2-ethanediyl]] and alpha-(Z)-octadec-9-en-1-yl-omega-hydroxy-tri[oxy[(chloromethyl)-1,2-ethanediyl]]

Test material form:

other: Liquid

Details on test material:

- Name of test material (as cited in study report): See confidential informations
- Storage condition of test material: At room temperature

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge: Treatment plant receiving predominantly domestic sewage (>98%), location: Maxéville (France)
- Storage conditions: Sampling date: 2013-03-19
- Preparation of inoculum for exposure: The sludge is centrifuged (1100 g for 10 minutes), the supernatant liquid is discarded and the sludge is re-suspended in the mineral medium and filtered (100µm). The washing and centrifuging process is repeated 3 times . Activated sludges were used within 24 h after sampling.
- Concentration of sludge: < 30 mg dry matter/L
- Water filtered: yes
- Type and size of filter used, if any: Inox sieve (100µm)

Duration of test (contact time):

ca. 28 d

Initial test substance concentration

Initial conc.:

ca. 42.2 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Culturing apparatus: flask 1L
- Composition of medium: 10 mL of solution A + 1mL of solutions B, C and D, completed to 1L with ultra-pure water
Preparation of solution A: 8.5 g KH2PO4, 21.75 g K2HPO4, 33.4 g Na2HPO4.2H2O, 0.5 g NH4Cl per liter of ultra-pure water,
Preparation of solution B: 27.5 g CaCI2 per liter of ultra-pure water
Preparation of solution C: 22.5 g MgSO4.7H2O per liter of ultra-pure water
Preparation of solution D: 0.25 g FeCI3.6H2O per liter of ultra-pure water.
- Source/preparation of dilution water: ultra-pure water
- Test temperature: 20-24 °C
- pH (at start of test): no data
- Continuous darkness: Not specified

TEST SYSTEM
- Number of culture flasks/concentration: 5 bottles containing only inoculum, 3 bottles containing inoculum and test substance, and 3 bottles containing inoculumand sodium acetate
- Measuring equipment: Respirometer CES (measure of the O2)
- Test performed in closed vessels: Yes
- Preparation of flask: the test material is directly added in the flask conatining the mineral medium and the inoculum

SAMPLING
- Sampling method: Direct measure in the flasks

CONTROL AND BLANK SYSTEM
- Inoculum blank: flask containing mineral medium and inoculum
- Reference substance: flask containing mineral medium, inoculum and sodium acetate
- Abiotic sterile control: no
- Toxicity control: no

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

Preliminary study:

No preliminary test

Test performance:

ThDO = 2.37 mg O2/mg test item

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

ca. 14

Sampling time:

4 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

ca. 50

Sampling time:

14 d

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

ca. 60

Sampling time:

24 d

% Degradation 4

Parameter:

% degradation (O2 consumption)

Value:

ca. 66

Sampling time:

28 d

Details on results:

The 10-day window for the test substance started on Day 4 and the test substance attained 50% of biodegradation on Day 14.
The oxygen consumption in the control was 29.3 mg at the test end.
The pH was 7.4 at the end of the test.

BOD5 / COD results

Results with reference substance:

The median percentage of degradation of the reference substance was 77% after 14 d.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable, but failing 10-day window

Conclusions:

Under the conditions of this study, the test substance, which doesn't satisfy the 10-d window and exhibits 66% of biodegradation after 28 days, is considered as readily biodegradable, but failing 10-day window, in accordance with the classification system of the EU (Directive 67/548/EC).

Executive summary:

The aerobic biodegradation of the test item was studied in a ready biodegradation test according to OECD Guideline 301F. Test vessels consisted of flasks filled in with 1L of test suspension. Sewage sludge was used as biological inoculum. Sewage sludge was sampled on a waste water treatment plant feeding on domestic sewage. The medium composition was compliant with OECD Guideline requirements. The following treatments were applied: inoculum blank (biological inoculum only), test item (biological inoculum + test substance) and procedure control (reference substance + biological inoculum). Acetic acid, sodium salt was used as reference substance. The concentration of the test item was 42.2 mg/L, corresponding to a ThDO of 100 mg/L

Test vessels were incubated for 28 days at 20-24°C. The O2 consumption was measured by automatic respirometer CES.

The biodegradation of the test item was ca. 13% after 4 days and reached 50% after 14 days and 66% after 28 days.

Under the test conditions, the test item must be regarded as readily biodegradable but failing 10 -day window.