Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-350-4 | CAS number: 105-99-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 1981
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Only secondary literature. Original report not available.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: secondary effluent, sewage, domestic, non-adapted
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Reference substance:
- not specified
- Parameter:
- % degradation (DOC removal)
- Value:
- 96
- Sampling time:
- 28 d
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Only secondary literature. Original report not available.
- Qualifier:
- according to guideline
- Guideline:
- ISO Draft (BOD Test for Insoluble Substances)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Duration of test (contact time):
- 28 d
- Parameter followed for biodegradation estimation:
- O2 consumption
- Reference substance:
- not specified
- Parameter:
- % degradation (O2 consumption)
- Value:
- > 95
- Sampling time:
- 28 d
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 Oct - 01 Nov 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP-Guideline study with acceptable restrictions.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- yes
- Remarks:
- Test duration was 14 days.
- Qualifier:
- according to guideline
- Guideline:
- other: Circular on Test Methods of New Chemical Substances (Japan), Biodegradation test
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: Mixture of activated sludge from STP with freshwater from rivers and lakes, marine water and marine sediment
- Details on inoculum:
- - Source of inoculum/activated sludge: Activated sludge was sampled from 4 sewage plants, freshwater (surface water) was sampled from 3 rivers and one lake and marine water and sediment was sampled from two bays in Japan.
- Laboratory culture: Thirty minutes after stopping of aeration, discard of about one third of the whole volume of supernatant and addition of an equal volume of a solution (pH adapted to 7.0 with NaOH) containing 5% each of glucose, peptone and potasium orthophosphate, to the settled material; restart with aeration. This procedure was repeated once per day.
- Temperature: 25 ± 2 °C
- Storage length: about one and a half month
- Preparation of inoculum for exposure: Activated sludge was mixed with old sludge which was used before.
- Concentration of sludge: 4200 mg/L - Duration of test (contact time):
- 14 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: as recommended in the guideline
- Test temperature: 25 ± 1 °C
- Suspended solids concentration: 30 mg/L
- Continuous darkness: not stated
TEST SYSTEM
- Culturing apparatus: closed oxgen consumption measuring machine with 300 mL culture bottle
- Number of culture flasks/concentration: 1 (water + test substance), 3 (sludge + test sub.), 1 (sludge + reference sub.), 1 (sludge)
- Measuring equipment: coulometer
- Details of trap for CO2 and volatile organics if used: soda lime
SAMPLING
- Sampling frequency: continuously until 14 days
- Sterility check if applicable: no
- Sample storage before analysis: not stated
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes - Reference substance:
- aniline
- Parameter:
- % degradation (O2 consumption)
- Value:
- 90
- Sampling time:
- 14 d
- Details on results:
- No 10-day window required. Substance was readily biodegradable.
- Results with reference substance:
- The oxygen consumption for aniline in the above used test system indicated 62.7% and 73.7% degradation after 7 days and 14 days, respectively.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 18 Feb - 18 Mar 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study with acceptable restriction: Several basic information about the study design were not stated, but it was referred to the conformity with internal SOPs.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Activated sludge was collected from a waste water treatment plant which predominately treats municipal waste water; Wupperverband, Germany; date of collection: 12 Feb 1999
- Preparation of inoculum for exposure: Inoculum was aerated for 6 days.
- Pretreatment: none
- Water filtered: yes
- Type and size of filter used, if any: coarse folded filter - Duration of test (contact time):
- 28 d
- Initial conc.:
- 2.62 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral medium
- Test temperature: 20 ± 1 °C
- pH: 7.4
- Continuous darkness: yes
TEST SYSTEM
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Inoculum was aerated for 6 days.
- Measuring equipment: O2 measuring advice with electrode
SAMPLING
- Sampling frequency: Samples were taken after 0, 7, 14, 21 and 28 days test duration.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2
- Toxicity control: 2 - Reference substance:
- aniline
- Remarks:
- 2 mg/L
- Parameter:
- % degradation (O2 consumption)
- Value:
- 60
- St. dev.:
- 1.4
- Sampling time:
- 28 d
- Details on results:
- The test substance was degraded to 60% within 28 days test duration. However, the 10 d-window was not passed. Hence, the test substance is considered to be biodegradable but not readily biodegradable according to OECD criteria.
- Parameter:
- COD
- Value:
- 2 291 mg O2/g test mat.
- Results with reference substance:
- The reference substance was degraded to 88% in 14 days and 98% in 28 days test duration.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: biodegradable
Referenceopen allclose all
Table 1. Result of analysis for BOD, HPLC and GC
|
water + test sub. |
sludge + test sub. |
Theoretical amount |
|||
sample No. 4 |
sample No. 1 |
sample No. 2 |
sample No. 3 |
|||
BOD (*1) |
mg |
0.2 |
66.4 |
61.6 |
68.6 |
68.7 |
GC (residue of test substance) |
mg |
28.2 |
0.0 |
0.0 |
0.0 |
30.3 |
% (*2) |
93 |
0 |
0 |
0 |
- |
|
HPLC (production of adipic acid from test water) |
mg |
1 |
0 |
0 |
0 |
17.1 |
% |
6 |
0 |
0 |
0 |
- |
|
HPLC (production of adipic acid from soda lime) |
mg |
0 |
0 |
0 |
0 |
17.1 |
% |
0 |
0 |
0 |
0 |
- |
|
GC (production of 1-butanol) |
mg |
1.2 |
0.0 |
0.0 |
0.0 |
17.4 |
% |
7 |
0 |
0 |
0 |
- |
(*1) (sludge + test sub.) was calculated by substracting blank values.
(*2) Residue and production percentage was calculated as followed.
Reside percentage = Residue amount (mg) / Theoretical amount (mg) x 100
Table 2. Chart of BOD
|
day 7 |
day 14 |
|
BOD (mg) |
BOD (mg) |
Deg (%) |
|
Sludge + Test sub. |
55.8 |
65.6 |
89 |
Sludge + Test sub. |
58.3 |
69.3 |
95 |
Sludge + Test sub. |
54.8 |
63.5 |
86 |
Water + Test sub. |
0.0 |
0.0 |
- |
Control blank |
1.0 |
3.7 |
- |
Sludge + Aniline |
62.7 |
73.7 |
- |
Table 1: Test results
Treatment |
O2 consumption after n days [mg/L] |
|||
7 d |
14 d |
21 d |
28 d |
|
Test item T1 |
1.77 |
2.89 |
3.72 |
3.68 |
Test item T2 |
1.68 |
2.28 |
3.38 |
3.53 |
Degradation [%] T1 |
29 |
48 |
62 |
61 |
Degradation [%] T2 |
28 |
38 |
56 |
59 |
Mean [%] |
29 |
43 |
59 |
60 |
|
|
|
|
|
Reference R1 |
2.76 |
4.36 |
4.70 |
4.71 |
Reference R2 |
2.71 |
4.15 |
4.62 |
4.67 |
Degradation [%] R1 |
57 |
90 |
98 |
98 |
Degradation [%] R2 |
56 |
86 |
96 |
97 |
Mean [%] |
57 |
88 |
97 |
98 |
|
|
|
|
|
Toxicity control 1 |
5.93 |
6.97 |
8.85 |
8.77 |
Toxicity control 2 |
5.20 |
4.75 |
8.85 |
8.77 |
Degradation [%] R1 |
55 |
64 |
82 |
81 |
Degradation [%] R2 |
48 |
44 |
82 |
81 |
Mean [%] |
52 |
54 |
82 |
81 |
Description of key information
Readily biodegradable: 90% (O2 consumption) in 14 days (OECD 301C)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
A reliable study investigating the ready biodegradability of Dibutyl adipate (CAS 105-99-7) is available. The study was conducted by Japanese authorities according to OECD 301C under aerobic conditions using mixtures of activated sludge from STP with freshwater from rivers and lakes, marine water and marine sediments (METI, 1995). Based on oxygen consumption a mean degradation of 90% was observed already after 14 days. Hence, Dibutyl adipate (CAS 105-99-7) is determined to be readily biodegradable according to OECD criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.