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reaction mass of: 7-amino-3,8-bis-[4-(2-sulfoxyethylsulfonyl)phenylazo]-4- hydroxynaphthalene-2-sulfonic acid, Na/K salt; 7-amino-3-[4-(2-sulfoxyethylsulfonyl)phenylazo]-4-hydroxy-8-[4-(2-sulfoxyethylsulfonyl)-2- sulfophenylazo]naphthalene-2-sulfonic acid, Na/K salt; 7-amino-8-[4-(2-sulfoxyethylsulfonyl)-phenylazo]-4-hydroxy-3-[4-(2-sulfoxyethylsulfonyl)- 2-sulfophenylazo]naphthalene-2-sulfonic acid, Na/K salt; 7-amino-3,8-bis-[4-(2-sulfoxyethylsulfonyl)-2-sulfophenylazo]-4-hydroxynaphthalene-2- sulfonic acid, Na/K salt
EC number: 429-070-4 | CAS number: 214362-06-8 SCARLET DER 8107
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- Analytical determinations and new study director
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Swiss GLP
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: TVR50
- Expiration date of the lot/batch: Sep 2004
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
- Solubility of the test substance in the solvent/vehicle: > 80 g/l (in water) - Analytical monitoring:
- yes
- Details on sampling:
- During the test the actual test concentrations in the analysed test media were in the range of 96.3 - 98.1% of the nominal values. The results are related to the nominal values of the test substance concentrations.
- Details on test solutions:
- Stock solution 300.0 mg test substance was mixed with about 200 ml test medium in a beaker, then homogenised by ultrasonic treatment for 2 minutes and filled up to 3000 ml with test medium.
Test concentrations: Due to the results of a pre-test the nominal test concentrations were chosen as 100 mg test substance/l. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- The test organism was the species zebrafish (Brachydanio rerio) supplied by Charles River Aquatics, Sorneren, Netherlands. The quality of the fish was checked with an acclimatization time of 89 days in dechlorinated tap water.
For each concentration, 7 fish were used. The fish were not fed during the test.
Weight of fish : 0.62 g/fish (average)
Length of fish : 26 mm (average)
Treatment : none - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Hardness:
- 142 mg CaCO3/L
- Test temperature:
- 21.1 - 21.4 °C
- pH:
- 7.9 - 8.4
- Dissolved oxygen:
- 95 - 98%
- Nominal and measured concentrations:
- During the test the actual test concentrations in the analysed test media were in the range of 96.3 - 98.1% of the nominal values. The results are related to the nominal values of the test substance concentrations.
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Neither mortality nor symptoms regards swimming behaviour, loss of equilibrium or repsiratory function nor pigmentation was observed in fist exposed to FAT40571/A.
- Results with reference substance (positive control):
- not applicable
- Reported statistics and error estimates:
- Due to lack of effects at limi concentration no statistics was applied.
- Sublethal observations / clinical signs:
Due to the results of a pre-test the nominal concentration of FAT 40571/A for the main test was selected as 100 mg test substance/1. Additionally, a blank control without test substance was tested.
During the test the actual test concentration in the analysed test media was in the range of 96.3 - 98.1 % of the nominal values. Therefore, the EC-values were determined from the nominal test concentration.
The LC50 value after 96 hours was determined to be >100 mg/l.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 and NOEC of FAT 40571/A after 96 h (zebrafish) was determined to be >100 mg/l
- Executive summary:
Acute toxicity of FAT 40571/A for zebrafish was carried out according to OECD 203 and EU C1 guideline to determine the LC50 (concentration at which 50% of the fish population died).
In total 7 fish at each test concentration (100 mg test substance / 1 nominal) used in this static test at 21.1 - 21.4°C and tested for 96 h. Analysis was carried out using Spectophotometry.
During the test the actual test concentrations in the analysed test media were in the range of 96.3 - 9 8 . 1% of the nominal values. The results are related to the nominal values of the test substance concentrations.
The LC50 value after 96 hours was determined to be >100 mg/1. So, in conclusion, the ecotoxicological classification of FAT 40571/A is not toxic to zebrafish after an exposure period of 96 h.
Reference
Description of key information
The LC50 and NOEC of Reactive Brown 49 after 96 h (zebrafish) was determined to be >100 mg/l
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Acute toxicity of Reactive Brown 49 for zebrafish was carried out according to OECD 203 and EU C1 guideline to determine the LC50 (concentration at which 50% of the fish population died).
In total 7 fish at each test concentration (100 mg test substance / 1 nominal) used in this static test at 21.1 - 21.4°C and tested for 96 h. Analysis was carried out using Spectophotometry.
During the test the actual test concentrations in the analysed test media were in the range of 96.3 - 98.1% of the nominal values. The results are related to the nominal values of the test substance concentrations.
The LC50value after 96 hours was determined to be >100 mg/l. So, in conclusion, the ecotoxicological classification of Reactive Brown 49 is not toxic to zebrafish after an exposure period of 96 h.
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