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EC number: 907-706-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- Experimental data of test chemical
- Justification for type of information:
- Experimental data for test chemical is from handbook or collection of data
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- WoE report is prepared based on long term toxicity to fish study:
1 and 2nd study - GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Details on test solutions:
- 2 study: Details on test solutions
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Determination of MIC was by the broth dilution method - Test organisms (species):
- other: 1: Colletotrichum musae DAR 24962, 2: Bacillus subtilis
- Details on inoculum:
- 1: The surface-plated cultures of the decay fungi in plastic Petri dishes were sub-cultured by streaking the spores onto the new potato dextrose agar (PDA) media. The plated cultures were then incubated for 7 days at 25°C.
The spores of 7-day-old cultures of decay fungi dislodged by sterile distilled water to which 0.1 mL/L of Tween 80 had been added. The spores were then filtered with sterile Sinta Glass No. 1 (Gallenkamp, London) to remove debris such as mycelia and condensed agar fragments, and the aliquot was diluted to a concentration of 10(5) fungal spores/mL suspensions. The fungal spore suspensions, 0.1 mL each, were then dispensed into Petri dishes (9-cm diam.) containing agar medium (PDA). The Petri dishes were then incubated for 3 days for the fungal cultures at 25 °C, to allow the spores to grow.
2nd study: Details on test organisms:
2-day-old cultures of the microorganisms were used in the test - Test type:
- other: 1: static, 2: broth dilution method
- Water media type:
- freshwater
- Total exposure duration:
- 10 d
- Post exposure observation period:
- 1. Agar disks of microorganism which failed to grow due to MIC were transferred onto new agar media free from test chemical and incubated for further 5 days at 25°C.
2: 2-5 days - Test temperature:
- 25°C
- Nominal and measured concentrations:
- 1. 4.65 mmol/dish (equivalent to 894.195 mg/L/dish)
- Details on test conditions:
- 1. Agar plugs (5.5-mm diam.) were picked up from the 3-day-old cultures of decay fungi using the bottom end of a sterilized Pasteur pipet and then transferred ontothe centers of new PDA media, in 9-cm plastic Petri dishes. The Petri dishes were then inverted and 7-cm Whatman No. 1 filter papers were attached onto the inner surface of their lids.
Ethanol, the first tested volatile in this experiment, was impregnated into the filter paper with varying volumes from 0.1 to 1.0 mL/dish in the 4°C room. Immediately after the impregnation, the Petri dishes were sealed by wrapping them with plastic film and incubated for 10 days at 25 °C. Experiments were repeated two timeswith four replications for each experiment. The minimum concentration of ethanol (expressed as mmol/dish) required to give complete control or the minimum inhibitory concentration (MIC) for each microorganism was determined.
The MIC of ethanol for each target decay microorganism was used as the initial level to identify the MIC of other tested volatiles. If the MIC level of ethanol used for other volatiles failed to stop the growth of pathogen, the level was increased until the MIC was found. However, if the volume of 1.5 mL/dish still failed to stop the growth of pathogen, the compound was considered ineffective as a vapor to stop the growth of pathogens. When the tested compounds had the same effect as the MIC of ethanol, the concentration was decreased until the MIC of the compound for each microorganism was determined. All the unit concentrations of MIC were then expressed as mmol/dish. - Reference substance (positive control):
- yes
- Remarks:
- 1: Ethanol
- Key result
- Duration:
- 10 d
- Dose descriptor:
- other: minimum inhibitory concentration (MIC)
- Effect conc.:
- 894.195 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: 1st study
- Key result
- Duration:
- 5 d
- Dose descriptor:
- other: MIC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: 2ND STUDY
- Details on results:
- 1. Test chemical exhibited fungistatic activity
- Results with reference substance (positive control):
- 1. Ethanol was considered germistatic against C. musae with MIC of 16.59 mmol/dish
- Validity criteria fulfilled:
- not specified
- Conclusions:
- 1. The Minimum Inhibitory Concentration (MIC) value of test chemical on the fungi Colletotrichum musae DAR 24962 was determine to be 894.195 mg/L.
2. Based on the antimicrobial effect of test chemical on the growth of Bacillus subtilis after the exposure of chemical for 2-5 days, the MIC was observed at 100 mg/l.
Thus based on the above studies, MIC ranges from 100 mg/l to 894.195 mg/l after the exposure of microorganisms with the test chemical for 2-10 days. - Executive summary:
Based on the various experimental data for the test chemical study have been reviewed to determine the toxic nature of test chemical on the growth of microorganisms. The studies are as mentioned below:
The effects of test chemical on the growth of Colletotrichum musae on agar medium were evaluated. Test was performed on the agar medium. Agar plugs (5.5-mm diam.) were picked up from the 3-day-old cultures of decay fungi using the bottom end of a sterilized Pasteur pipet and then transferred onto the centers of new PDA media, in 9-cm plastic Petri dishes. The Petri dishes were then inverted and 7-cm Whatman No. 1 filter papers were attached onto the inner surface of their lids. Ethanol, the first tested volatile in this experiment, was impregnated into the filter paper with varying volumes from 0.1 to 1.0 mL/dish in the 4°C room. Immediately after the impregnation, the Petri dishes were sealed by wrapping them with plastic film and incubated for 10 days at 25 °C. Experiments were repeated two times with four replications for each experiment. The minimum concentration of ethanol (expressed as mmol/dish) required to give complete control or the minimum inhibitory concentration (MIC) for each microorganism was determined. The MIC of ethanol for target decay microorganism was used as the initial level to identify the MIC of other tested volatiles. If the MIC level of ethanol used for other volatiles failed to stop the growth of pathogen, the level was increased until the MIC was found. However, if the volume of 1.5 mL/dish still failed to stop the growth of pathogen, the compound was considered ineffective as a vapor to stop the growth of pathogens. When the tested compounds had the same effect as the MIC of ethanol, the concentration was decreased until the MIC of the compound for each microorganism was determined. All the unit concentrations of MIC were then expressed as mmol/dish. After the incubation of 10 days, the Minimum Inhibitory Concentration (MIC) value of test chemical on the fungi, Colletotrichum musae DAR 24962 was determine to be 894.195 mg/L.
First study was supported by the second experimental study. Aim of this study was to evaluate the effect of test chemical on the growth of Bacillus subtilis and other fungi. The antimicrobial activity of test compounds against various bacteria and fungi was examined by the broth dilution method. Solution of the test compound was added to 2-day-old cultures of the microorganisms. After 2-5 days of incubation, growth of the microorganisms was checked. Minimal inhibitory concentrations (MICs) were measured by two fold serial broth dilution. Based on the antimicrobial effect of test chemical on the growth of Bacillus subtilis after the exposure of chemical for 2-5 days, the MIC was observed at 100 mg/l.
Thus based on the above studies, MIC ranges from 100 mg/l to 894.195 mg/l after the exposure of microorganisms with the test chemical for 2-10 days.
Reference
Description of key information
1. The Minimum Inhibitory Concentration (MIC) value of test chemical on the fungi Colletotrichum musae DAR 24962 was determine to be 894.195 mg/L.
2. Based on the antimicrobial effect of test chemical on the growth of Bacillus subtilis after the exposure of chemical for 2-5 days, the MIC was observed at 100 mg/l.
Thus based on the above studies, MIC ranges from 100 mg/l to 894.195 mg/l after the exposure of microorganisms with the test chemical for 2-10 days.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
Additional information
Based on the various experimental data for the test chemical and structually and functionally similar read across chemicals, study have been reviewed to determine the toxic nature of test chemical on the growth of microorganisms. The studies are as mentioned below:
The effects of test chemical on the growth of Colletotrichum musae on agar medium were evaluated. Test was performed on the agar medium. Agar plugs (5.5-mm diam.) were picked up from the 3-day-old cultures of decay fungi using the bottom end of a sterilized Pasteur pipet and then transferred onto the centers of new PDA media, in 9-cm plastic Petri dishes. The Petri dishes were then inverted and 7-cm Whatman No. 1 filter papers were attached onto the inner surface of their lids. Ethanol, the first tested volatile in this experiment, was impregnated into the filter paper with varying volumes from 0.1 to 1.0 mL/dish in the 4°C room. Immediately after the impregnation, the Petri dishes were sealed by wrapping them with plastic film and incubated for 10 days at 25 °C. Experiments were repeated two times with four replications for each experiment. The minimum concentration of ethanol (expressed as mmol/dish) required to give complete control or the minimum inhibitory concentration (MIC) for each microorganism was determined. The MIC of ethanol for target decay microorganism was used as the initial level to identify the MIC of other tested volatiles. If the MIC level of ethanol used for other volatiles failed to stop the growth of pathogen, the level was increased until the MIC was found. However, if the volume of 1.5 mL/dish still failed to stop the growth of pathogen, the compound was considered ineffective as a vapor to stop the growth of pathogens. When the tested compounds had the same effect as the MIC of ethanol, the concentration was decreased until the MIC of the compound for each microorganism was determined. All the unit concentrations of MIC were then expressed as mmol/dish. After the incubation of 10 days, the Minimum Inhibitory Concentration (MIC) value of test chemical on the fungi, Colletotrichum musae DAR 24962 was determine to be 894.195 mg/L.
First study was supported by the second experimental study. Aim of this study was to evaluate the effect of test chemical on the growth of Bacillus subtilis and other fungi. The antimicrobial activity of test compounds against various bacteria and fungi was examined by the broth dilution method. Solution of the test compound was added to 2-day-old cultures of the microorganisms. After 2-5 days of incubation, growth of the microorganisms was checked. Minimal inhibitory concentrations (MICs) were measured by two fold serial broth dilution. Based on the antimicrobial effect of test chemical on the growth of Bacillus subtilis after the exposure of chemical for 2-5 days, the MIC was observed at 100 mg/l.
Thus based on the above studies, MIC ranges from 100 mg/l to 894.195 mg/l after the exposure of microorganisms with the test chemical for 2-10 days.
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