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EC number: 617-251-2 | CAS number: 816431-72-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06 Mar - 18 Apr 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: aeration tank of a wastewater plant treating predominantly domestic sewage (Wupper area water authority, STP Odenthal)
- Pretreatment: none
- Concentration of sludge: 30mg/Lss - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral medium
- Test temperature: 22 ± 2°C
- pH: 7.2
- pH adjusted: no
- Continuous darkness: yes
TEST SYSTEM
- Test apparatus: Oxi Top System (WTW)
- Number of culture flasks/concentration: 3 (but only 2 used for calculation)
- Measuring equipment: The consumption of oxygen is determined by measuring the
quantity of oxygen (produced electrolytically) required to maintain constant gas volume in the
respirometer flask.
- Details of trap for CO2 and volatile organics if used: Evolved carbon dioxide is absorbed in a solution of potassium hydroxide
SAMPLING
- Sampling frequency: Day 2, 6, 8, 10, 14, 16, 20, 22, 24 and 28
- Sampling method: not specified
- Sample storage before analysis: not specified
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: yes
- Reference control: yes - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- Oxygen uptake by nitrification has been determined within the study. The oxygen consumed by
nitrification was 2 mg/L. This oxygen consumption by nitrification was subtracted from the
respective 28 days measurements of the test item. Results were presented as BOD values which were corrected for the oxygen consumed by nitrification. - Results with reference substance:
- The reference compound showed 85 % degradation after 14 days.
The toxicity control showed 45% degradation after 14 days. - Validity criteria fulfilled:
- yes
- Remarks:
- See more details in the table in the section "Any other information on results incl. tables"
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Within 28 days, a degradation of 0 % was determined for the test substance.
- Executive summary:
The ready biodegradability of the test substance was assessed in accordance with with the Council Directive 92/69/EEC Method C.4-D "Manometric Respirometry Test". The test substance in a mineral medium was inoculated with activated sludge and incubated for 28 d under aerobic conditions. The consumption of oxygen was determined by measuring the quantity of oxygen (produced electrolytically) in the respirometer flask. The oxygen demand values of the test item after 28 days were corrected for the oxygen consumed by nitrification. Percentage biodegradation of the test substance was 0% after 28 days and the reference compound showed 85 % degradation after 14 days. Therefore, the test substance is considered to be "Not Readily Biodegradable".
Reference
Validity criteria for the measurement of the biodegradation:
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
Normally, the test lasts for 28 days. Tests however may be ended before 28 days, i.e. as soon as the biodegradation curve has reached a plateau for at least three determinations. Tests may also be prolonged beyond 28 days when the curve shows that biodegradation has started but that the plateau has not been reached by day 28, but in such cases the chemical would not be classed as readily biodegradable. | The test was conducted for 28 days | Yes |
In order to check the procedure, reference compounds which meet the criteria for ready biodegradability are tested by setting up an appropriate vessel in parallel as part of normal test runs. Suitable compounds are aniline (freshly distilled), sodium acetate and sodium benzoate. | Sodium benzoate was tested as reference substance | Yes |
A test is considered valid if the difference of extremes of replicate values of the removal of test chemical at the plateau, at the end of the test or at the end of the 10 -day window, as appropriate, is less than 20% and if the percentage degradation of the reference substance has reached the level for ready biodegradability by 14 days. If either of these conditions is not met, the test should be repeated. | Out of 3 replicates used for the test, 2 replicates are within 20% variation but values for third replicate exceeded 20% variation. However, this was not used for the calculation of biodegradation since only 2 replicates are required by the guideline. | Yes |
If in a toxicity test, containing both the test substance and a reference compound, less than 35% degradation (based on total DOC) or less than 25% (based on total ThOD or ThCO2) occurred within 14 days, the test substance can be assumed to be inhibitory. | Percentage degradation in the toxicity control was 46% at day 14. | Yes |
The oxygen uptake of the inoculum blank is normally 20-30 mg 02/L and should not be greater than 60 mg/l in 28 days. Values higher than 60 mg/L require critical examination of the data and experimental technique. | The oxygen uptake of the inoculum blank is < 60 mg/L. | Yes |
Description of key information
The ready biodegradability of the test substance was assessed in accordance with with the Council Directive 92/69/EEC Method C.4-D "Manometric Respirometry Test". The test substance in a mineral medium was inoculated with activated sludge and incubated for 28 d under aerobic conditions. The consumption of oxygen was determined by measuring the quantity of oxygen (produced electrolytically) in the respirometer flask. The oxygen demand values of the test item after 28 days were corrected for the oxygen consumed by nitrification. Percentage biodegradation of the test substance was 0% after 28 days and the reference compound showed 85 % degradation after 14 days. Therefore, the test substance is considered to be "Not Readily Biodegradable".
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.