Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 427-820-5 | CAS number: 2701-50-0 1,2-METHYLEN-4,6-DIENACETAT
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 Apr - 03 Sep 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: activated sewage sludge from a municipal teatment plant (Kläranlage Berlin Ruhleben)
- Preparation of inoculum for exposure: effluent concentration in reaction mixture was 5 mL/L
- Pretreatment: the activated sludge was stirred and aerated for about 2 h, then homogenized and settled for 30 min.
- Water filtered: yes - Duration of test (contact time):
- 28 d
- Initial conc.:
- 10 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 28 d
- Remarks on result:
- other: nominal concentration: 20 mg/L
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 2
- Sampling time:
- 28 d
- Remarks on result:
- other: nominal concentration: 10 mg/L
- Results with reference substance:
- The reference compound sodium acetate was degraded to more than 80%.
- Validity criteria fulfilled:
- yes
- Remarks:
- See 'Any other information on results incl. tables'
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The reference compound sodium acetate was degraded to more than 80%, the test substance was degraded to 2% and 5%, respectively. The test substance is not readily biodegradable under the conditions of the test.
- Executive summary:
The study was conducted to assess the biodegradation in water: ready biodegradability. The study was conducted in accordance with the OECD guideline 301 B. The reference compound sodium acetate was degraded to more than 80%, the test substance was degraded to 2% and 5%, respectively. The test substance is not readily biodegradable under the conditions of the test.
Reference
Validity criteria for the measurement of the biodegradation according to OECD 301:
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
In order to check the procedure, reference compounds which meet the criteria for ready biodegradability are tested by setting up an appropriate vessel in parallel as part of normal test runs. Suitable compounds are aniline (freshly distilled), sodium acetate and sodium benzoate. | sodium acetate | Yes |
A test is considered valid if the difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20% and if the percentage degradation of the reference compound has reached the pass levels by day 14. | The % degradation of the two measured values of the test substance at the end of the test (28 d) range between 2 -5% There was 73% degradation of the reference substance after 28 d - the pass level (88 % for ThOD) was reached. | Yes |
If in a toxicity test, containing both the test substance and a reference compound, less than 35% degradation (based on total DOC) or less than 25% (based on total ThOD or ThCO2) occurred within 14 days, the test substance can be assumed to be inhibitory. The test series should be repeated, using a lower concentration of test substance (if this can be done without seriously impairing the accuracy of the DOC determination) and/or a higher concentration of inoculum, but not greater than 30 mg solids/L. | The reference substance sodium acetate was degraded to more than 60% within 9 day. The CO2 produced in the blank control (35 mg) in the normal test period was within the limit set by the OECD guideline (50 mg). Hence, the quality criteria of the OECD guideline were fulfilled, i.e., the inoculum was viable and active. | Yes |
Description of key information
The study was conducted to assess the ready biodegradability. The study was conducted in accordance with the OECD guideline 301 B. The reference compound sodium acetate was degraded to more than 80%, the test substance was degraded to 2% and 5%, respectively. The test substance is not readily biodegradable under the conditions of the test.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.