Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 433-460-1 | CAS number: 210880-92-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01 December 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- TI-435 Technical was not considered sufficiently soluble in water to facilitate dosing from a concentrated aqueous stock solution.
The quantities of TI-435 Technical required were weighed onto strips of aluminium foil and the strips and the test substance added to the respective test vessels which contained reverse-osmosis water and synthetic sewage. The vessels were placed in an ultrasonic bath. The contents of the vessels were subjected to ultrasonication for approximately 10 minutes to disperse the test substance homogeneously in the test medium before adding the inoculum.
Each aliquot of synthetic sewage was amended with water and the appropriate weight of test substance or volume of the reference substance stock solution. The test mixtures were completed sequentially at timed intervals by adding equal volumes of inoculum. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- A sample of activated sludge was collected from one of the return lines at Burley Menston sewage treatment works (Yorkshire Water).
The initial suspended solids concentration was 4.4040 g/L and lay above the permitted limits of 4 g ± 10% per litre. A portion was therefore diluted with de-chlorinated tap water to provide 8 L inoculum with a nominal solids content of 4 g/L. The pH of the
inoculum after dilution was 7.60 and lay within the acceptable range of pH 6 to 8. Further initial adjustment was therefore unnecessary.
The inoculum was used in the range-finder two days after the sludge sample was collected. The inoculum was maintained by aeration at all times and was fed overnight with 400 mL synthetic sewage on two occasions during the interval between collection and use. - Test type:
- static
- Water media type:
- other: de-chlorinated tap water
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- Not specified
- Test temperature:
- 20.9 - 21.6°C
- pH:
- 5.91 and then raised to 6.51
- Dissolved oxygen:
- No indication
- Salinity:
- Not applicable
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Nominal: 1, 10, 100 and 1000 mg test item/L
- Details on test conditions:
- The inoculum was used in the range-finder two days after the sludge sample was collected. The inoculum was maintained by aeration at all times and was fed overnight with 400 mL synthetic sewage on two occasions during the interval between collection and use.
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-DCP
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- TI-435 Technical did not inhibit the respiration of activated sludge at concentrations up to and including 1000 mg/L. The concentration of TI-435 Technical causing a 50% reduction in respiration rate, relative to untreated controls, could not be determined from the results of this study, but it exceeded the highest dose tested, i.e. EC50 > 1000 mg/L.
- Results with reference substance (positive control):
- Regression coefficient: 0.995
EC20: 3 mg/L
EC50: 8 mg/L
EC80: 26 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- The present guideline study was conducted in compliance with GLP. Under the test conditions used, the EC50 3h for activated sludge was > 1000 mg test item/L.
Reference
Please refer to "overall remark/ attached background material" field for result tables.
Table 1: Validity criteria for OECD 209 (2010)
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The blank controls (without the test substance or reference substance) oxygen uptake rate should not be less than 20 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour. |
Lowest consumption in control is 60.5 mg O2/L/hour considering 200 mL of 4 g dw activated sludge/L, the lowest O2 uptake= 37.81 mg O2/g dw sludge/hour |
Yes |
The coefficient of variation of oxygen uptake rate in control replicates should not be more than 30% at the end of definitive test. |
15% |
Yes |
EC50 of the reference substances has to lie in a specific range |
3,5-DCP: 8 mg/L (total respiration) |
Yes |
Description of key information
From the activated sludge key study, the 3-hour EC50 for microorganisms exposed to the present test material was > 1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
One key study (2000) is available for toxicity towards microorganisms. In this study, the effects of the substance towards microorganisms were examined in a 3-h toxicity test with activated sludge under static conditions. The study was carried out according to GLP and OECD guideline 209. Activated sludge was exposed to nominal concentrations of 1, 10,100 and 1000 mg/L, alongside with a control. Based on nominal concentrations an EC50 of > 1000 mg/L was determined.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.