Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 278-636-5 | CAS number: 77182-82-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 81-3 (Acute inhalation toxicity)
- GLP compliance:
- yes
- Test type:
- traditional method
Test material
- Reference substance name:
- Ammonium 2-amino-4-(hydroxymethylphosphinyl)butyrate
- EC Number:
- 278-636-5
- EC Name:
- Ammonium 2-amino-4-(hydroxymethylphosphinyl)butyrate
- Cas Number:
- 77182-82-2
- Molecular formula:
- C5H12NO4P.H3N
- IUPAC Name:
- ammonium 2-amino-4-[hydroxy(methyl)phosphoryl]butanoate
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Hoechst AG, Kastengrund, SPF breeding colony
- Age at study initiation: 8-10 weeks
- Weight at study initiation: males 163-193 g, females 174-193 g
- Housing: in fully air-conditioned rooms in Makrolon cages (Type 4) on softwood granulate in groups of 5 animals
- Diet (e.g. ad libitum): rat diet Altromin 1324 (Altromin GmbH, Lage/Lippe), ad libitum
- Water (e.g. ad libitum): tap water in plastic bottles, ad libitum
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 2
- Humidity (%): 50 +/- 20
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- inhalation: dust
- Type of inhalation exposure:
- nose only
- Vehicle:
- air
- Mass median aerodynamic diameter (MMAD):
- >= 0.6 - <= 10.3 µm
- Details on inhalation exposure:
- The rats were placed individually in cylindrical plastic tubes and exposed to specified dust concentrations for 4 hours. The plastic tubes leading into the exposure cylinder are so arranged that only the noses of the animals are inside the cylinder. The inhalation chamber itself consists of a stainless steel and glass cylinder with a volume of 60 L, standing in a vent pipe with a volume of ca. 4 m3. Particles of test substance escaping from the exposure
chamber into the vent pipe are drawn off and neutralized by gas-cleaning equipment.
The dust was produced with the aid of a "Wright Dust Feed" generator manufactured by L. Adams Ltd., London. The different concentrations were achieved by adjusting the gear transmission ratios. The dust produced by the scraper head was drawn from above into the exposure chamber by a current of air flowing at a rate of 1000 L/h at 4 bar.
A suction device at the bottom of the inhalation chamber drew off the dust at a rate of 1000 L/h through a cotton-wool filter and a washing bottle filled with water. - Remarks on duration:
- 4 hours
- Concentrations:
- 0.12, 0.19, 0.38, 2.0 mg/L
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: weighing took place on days 2, 3, 4, 7 and 14 after exposure
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, macroscopic examination, behavioural tests - Statistics:
- The LC 50, the 95 % range of confidence and the equation of the probit lines were assessed on the basis of the actual death rates by probit analysis (according to the method of UNDER and WEBER); the limits of confidence were calculated according to the method of FIELLER or SIDAK (program supplied by the Department Praktische Mathematik of HOECHST A6)
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- 1.26 mg/L air
- Sex:
- female
- Dose descriptor:
- LC50
- Effect level:
- 2.6 mg/L air
- Mortality:
- 0.12 mg/L: 0/10
0.19 mg/L: 1/10
0.38 mg/L: 2/10
2.0 mg/L: 5/10 - Clinical signs:
- other: The following clinical signs of intoxication were observed: narrowed eye openings, periodic tremors and clonic convulsions, hyperactivity, pilo-erection, increased salivation and passivity.
- Body weight:
- A reduction of bodyweights was recorded in the male and female animals during the days immediately after inhalation, to a greater extent with increasing concentrations. All surviving animals had regained their initial weight on day 14 after inhalation.
- Gross pathology:
- Autopsy of the animals which died during and of those killed at the end of the study revealed no macroscopically visible changes.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.