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EC number: 617-694-1 | CAS number: 85261-19-4
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vitro
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 May 2017- 19 Jun 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 442C (In Chemico Skin Sensitisation: Direct Peptide Reactivity Assay (DPRA))
- Version / remarks:
- February 2015
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Direct Peptide Reactivity Assay (DPRA) for Skin Sensitization Testing, DB-ALM Protocol n°154, January 12, 2013
- Version / remarks:
- January 2013
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- other: (in chemico) reactivity against synthetic peptides with a thiol or amino group
Test material
- Reference substance name:
- N-methyl-N-[(2S,3R,4R,5R)-2,3,4,5,6-pentahydroxyhexyl]nonanamide
- EC Number:
- 617-694-1
- Cas Number:
- 85261-19-4
- Molecular formula:
- C16H33NO6
- IUPAC Name:
- N-methyl-N-[(2S,3R,4R,5R)-2,3,4,5,6-pentahydroxyhexyl]nonanamide
- Test material form:
- solid
- Details on test material:
- sample no. MTDID 48740
MEGA 9: 98.1 % (GC)
Water content: 0.3%
pH (aqueous solution 1%): 7.5
Expiry Date: 2017-12
Product Nr: MTDID 48740
Batch: 549612
Constituent 1
- Specific details on test material used for the study:
- Test Item: MTDID 48740
Expiration: Dec 2017
Ptate/Purity:Solid/ 98.1%
Storage:RT
MW (g/mol): 335.44
Solvent: Water
In vitro test system
- Details on the study design:
- DPRA was developed by Frank Gerberick and colleagues (2004) and was further refined in 2007 (Gerberick et al., 2007), and a full OECD guideline for the assay was released in February of 2015. In this assay, the test article was incubated concurrently in two separate buffers, one with cysteine (Ac-RFAACAA-COOH) and one with lysine (Ac-RFAAKAA-COOH). Reactive chemicals bind one or both of the peptides thereby reducing their free concentration levels. The disappearance of each peptide is measured by HPLC/UV. This method does not require any biological material such as enzymes in order for this reaction to take place. It is important to note that the cysteine peptide captures soft electrophiles, while the lysine peptide captures hard electrophiles. This makes the DPRA assay a good choice to screen for reactive chemicals which are associated with allergic contact dermatitis.
Results and discussion
- Positive control results:
- Positive control, 2,3- butanedione (CAS 431-03-8), was prepared at 100 mM in acetonitrile (CAS 75- 05-8, Lot 160783).
In vitro / in chemico
Resultsopen allclose all
- Key result
- Run / experiment:
- other: cystein
- Parameter:
- other: mean peptide depletion [%]
- Value:
- 0.5
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Run / experiment:
- other: lysin
- Parameter:
- other: mean peptide depletion [%]
- Value:
- 2.1
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- In this study under the given conditions the test item showed no reactivity towards both peptides (cyten and lysin). The test item might be considered as non sensitiser.
- Executive summary:
The purpose of this study was to screen three test articles 48740 for their potential to act as chemical sensitizers using the Direct Peptide Reactivity Assay (DPRA), a test used to assay reactivity of test articles with small peptides according to OECD 442C.
Since the acceptance criteria for the depletion range of the positive control were fulfilled, the observed precipitation and phase separation was regarded as insignificant.
The 100 mM stock solution of the positive control (2,3 -butanedione) showed high reactivity towards the synthetic peptides.
Peptide depletion by 2,3-butandione is expected to be between 10 – 45% for the lysine (23.2%) peptide and 60 – 100% for the cysteine peptide (85.8%). The mean depletion by 2,3 -Butandione of both peptides was 54.5%.
The test Item (MTDID 48740) was found to have no/minimal reactivity with the peptides and was ranked as negative for
sensitization potential (% Cys Dep 0.5 and % lys Dep 2.1) (Mean depletion 1.3%).
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