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Diss Factsheets
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EC number: 284-902-1 | CAS number: 84989-13-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- Well-documented journal article.
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
Refer to Section 13.2 for read-across justification document.
Data source
Reference
- Reference Type:
- publication
- Title:
- The Cytogenetic Studies and Dominant Lethal Tests of Long Term Administration with Butylated Hydroxytoluene (BHT) and Linear Alkylbenzene Sulfonates (LAS) in Mice and Rats
- Author:
- Masubuchi, M, Takahashi, A, Takahashi, O, Hiraga, K
- Year:
- 1 976
- Bibliographic source:
- Ann. Rep. Tokyo Metr. Res. Lab. P.H. 27-2.
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- A group of 5 male mice was fed a diet containing 0.9% test substance for 9 months. At the end of this period, the animals were sacrificed, and the bone marrow cells examined for chromosome aberrations.
- GLP compliance:
- no
- Remarks:
- Study pre-dates GLP
- Type of assay:
- mammalian germ cell chromosome aberration test
Test material
- Details on test material:
- - Name of test material (as cited in study report): linear alkylbenzene sulfonate
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- other: JCL-ICR
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: CLEA Japan, Inc.
- Age at study initiation: 4 weeks
- Housing: individually, except during breeding
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period:
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 25 +/- 1
- Humidity (%): 55 +/- 5
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: feed
- Details on exposure:
- DIET PREPARATION
- Mixing appropriate amounts with (Type of food): feed powder CE-2 - Duration of treatment / exposure:
- 9 months
- Frequency of treatment:
- Daily
Doses / concentrations
- Dose / conc.:
- 1 170 mg/kg bw/day (nominal)
- Remarks:
- Basis: Nominal in diet
- No. of animals per sex per dose:
- 5
- Control animals:
- yes
Examinations
- Tissues and cell types examined:
- femur bone marrow cells
- Details of tissue and slide preparation:
- Animals were sacrificed by administration of 1 ml/kg of 1% colchine solution. Femurs were then removed, and bone marrow cells washed into centrifuge tubes. The cells were then treated with 0.075 M KCl solution at 37 degree C for 15 min, and then fixed with an acetic acid 1: ethanol 3 solution. Samples were then flame dried and treated with Giemsa.
- Evaluation criteria:
- Presence and absence of chromosomal aberrations. 50 metaphases per individual.
- Statistics:
- Rohrborn's method.
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- negative
- Negative controls validity:
- valid
- Remarks on result:
- other: No increase in chromosome aberrations was noted.
- Additional information on results:
- No increase in chromosome aberrations was noted.
Any other information on results incl. tables
Chromosome Aberrations
1170 mg/kg bw/d in diet | Control | |
Number of cells with chromatid breaks | 1 | 2 |
Number of cells with isochromatid breaks | 1 | 0 |
Number of cells with chromatid gaps | 4 | 5 |
Number of cells with isochromatic gaps | 0 | 0 |
Number of cells with other aberrations | 0 | 0 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: negative
The test substance is not clastogenic. - Executive summary:
A group of 5 male mice was fed a diet containing 0.9% test substance for 9 months. At the end of this period, the animals were sacrificed, and the bone marrow cells examined for chromosome aberrations. No increase in chromosome aberrations was seen as compared to controls. The test substance is not clastogenic.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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