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EC number: 602-927-1 | CAS number: 123312-89-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23 Jul 1991 to 6 Aug 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 81-2 (Acute Dermal Toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- 6-methyl-4-[(E)-[(pyridin-3-yl)methylidene]amino]-2,3,4,5-tetrahydro-1,2,4-triazin-3-one
- EC Number:
- 602-927-1
- Cas Number:
- 123312-89-0
- Molecular formula:
- C10H11N5O
- IUPAC Name:
- 6-methyl-4-[(E)-[(pyridin-3-yl)methylidene]amino]-2,3,4,5-tetrahydro-1,2,4-triazin-3-one
- Test material form:
- solid: particulate/powder
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Remarks:
- Hsd:Sprague Dawley SD
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 250 to 286 g (for males), 220 to 244 g (for females)
- Housing: individually housed in screen-bottom, stainless steel cages.
- Diet: a measured amount of Rodent Chow® #5001
- Water: ad libitum
- Acclimation period: at least 7 days
ENVIRONMENTAL CONDITIONS
Animal husbandry and housing comply with standards outlined in the "Guide for the Care and Use of Laboratory Animals" (NIH publication number No. 86-23, revised 1985). Variations from the prescribed environmental conditions existed and were considered to have had no effect on the study outcome. No contaminants were expected to have been present in the feed or water which would have interfered with or affected the results of the study.
- Temperature (°C): 19 to 25
- Humidity (%): 52 to 69
IN-LIFE DATES:
23 July 1991 to 6 August 1991
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- physiological saline
- Details on dermal exposure:
- TEST SITE
- Area of exposure: back of the rat
- % coverage: at least 10%
- Type of wrap if used: The area of application was covered with a 5.0 cm x 5.0 cm gauze patch secured with paper tape, and overwrapped with Saran Wrap and Elastoplast tape.
REMOVAL OF TEST SUBSTANCE
- Washing: washed using tap water and disposable paper towels
- Time after start of exposure: at the end of the 24-hour exposure period - Duration of exposure:
- 24 hours
- Doses:
- 2.0 g/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Clinical signs and mortality checks were conducted at approximately 1, 2.5, and 4 hours after test material administration. The animals were observed daily thereafter for 14 days for clinical signs and dermal irritation and twice a day (morning and afternoon) for mortality. Body weights were determined before test material application (Day 0), at Day 7, and at termination of the experimental phase (Day 14).
- Necropsy of survivors performed: yes - Statistics:
- No statistical analyses were required by the protocol.
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- no indication of skin irritation up to the relevant limit dose level
- Mortality:
- No mortality observed
- Clinical signs:
- other: All animals appeared clinically normal and exhibited no dermal irritation throughout the study
- Gross pathology:
- No deviations from normal morphology were found
Applicant's summary and conclusion
- Interpretation of results:
- other: Not classified according to CLP
- Conclusions:
- In this GLP compliant US EPA 81-2 study in Sprague-Dawley rats, the LD50 was determined to be > 2000 mg/kg body weight for males/females.
- Executive summary:
In this GLP compliant US EPA 81-2 study, the acute dermal toxicity of the test substance was evaluated in male and female Sprague-Dawley rats (5 animals per sex). The moistened test substance was administered as a single topical application under occlusive conditions at a level of 2000 mg/kg of body weight. After 24 hours of exposure, the test substance was washed using tap water and disposable paper towels. Clinical signs, body weight and mortality were recorded for 14 days after the end of the treatment. All animals were clinically normal and exhibited no dermal irritation throughout the study. Body weights were unaffected and no deviations from normal morphology were found. No animals died during the study. The estimated dermal LD50 was determined to be greater than 2000 mg/kg bw.
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