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EC number: 306-529-6 | CAS number: 97281-29-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From June 24, 2010 to August 19, 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Remarks:
- Spectrometry
- Details on sampling:
- Throughout the study (at 0, 24, 48, 72 and 96 h)
- Vehicle:
- no
- Details on test solutions:
- The test solution was prepared by directly dissolving appropriate amount of test substance in dilution medium and then facilitating its dispersion by ultrasonication for 30 min.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Source: Institute of hydrobiology, Chinese academy of sciences
Acclimation: 7 days
Photoperiod: 16 hours light
Oxygen concentration: > 80%
Diet: daily - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 119 - 122 mg CaCO3/L
- Test temperature:
- 23.0 - 23.1
- pH:
- 7.00 - 7.63
- Dissolved oxygen:
- 96 - 103% of the air saturation
- Salinity:
- -
- Conductivity:
- -
- Nominal and measured concentrations:
- 0 (negative control) to 50 mg/L
- Details on test conditions:
- Based on a range-finding test, a definitive test was performed.
Three replicates were assigned for each group
The number of fish was 10
During the definitive test, mortality, inactivity, strange swimming pattern and other abnormal behaviours were recorded.
pH, dissolved oxygen and temperature were recorded as well throughout the experiment. - Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 29.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: analysed concentration of 27.7 mg/L
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: analysed concentration of 19.2 mg/L
- Details on results:
- All fish in the control group were alive and appeared normal. The treated fishes were observed to be lying on side or back and abnormally swift swimming with the test concentration no less than 30 mg/L. Fishes treated with 50 mg/L test substance were also observed to be tumbling during swimming.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the 96 h LC50 and NOEC (in Danio rerio) of the test substance were determined to be 29.4 and 20 mg/L (nominal), corresponding to 27.7 and 19.2 mg/L (measured concentrations), respectively.
- Executive summary:
A study was conducted to determine the short-term toxicity of the test substance, 'di-C18-22 AAEMIM-MS' (active: 100%), to fish according to OECD Guideline 203, in compliance with GLP. Based on a range-finding test, a definitive test was performed with test substance concentrations of 0 to 50 mg/L under static conditions. Three replicates of 10 Danio rerio were assigned for each group. During the definitive test, mortality, inactivity, strange swimming pattern and other abnormal behaviours were recorded. pH, dissolved oxygen and temperature were measured as well throughout the experiment. Test substance concentrations were analysed by spectrometry at 0, 24, 48, 72 and 96 h and showed a good linearity when increasing the dose. All fish in the control group were alive and appeared normal. The treated fishes were observed to be lying on side or back and abnormally swift swimming with the test concentration no less than 30 mg/L. Fishes treated with 50 mg/L test substance were observed to be tumbling during swimming. Under the study conditions, the 96 h LC50 and NOEC of the test substance were determined to be 29.4 and 20 mg/L (nominal), corresponding to 27.7 and 19.2 mg/L (measured concentrations), respectively (Key Lab., 2010).
Reference
Description of key information
Based on the results of the study, the 96 h LC50 and NOEC of the test substance, di-C18-22 AAEMIM-MS, for toxicity to fish were determined to be 29.4 and 20 mg/L (nominal), corresponding to 27.7 and 19.2 mg/L (measured concentration), respectively.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 27.7 mg/L
Additional information
A study was conducted to determine the short-term toxicity of the test substance, 'di-C18-22 AAEMIM-MS' (active: 100%), to fish according to OECD Guideline 203, in compliance with GLP. Based on a range-finding test, a definitive test was performed with test substance concentrations of 0 to 50 mg/L under static conditions. Three replicates of ten Danio rerio were assigned for each group. During the definitive test, mortality, inactivity, strange swimming pattern and other abnormal behaviours were recorded. pH, dissolved oxygen and temperature were measured as well throughout the experiment. Test substance concentrations were analysed by spectrometry at 0, 24, 48, 72 and 96 h and showed a good linearity when increasing the dose. All fish in the control group were alive and appeared normal. The treated fishes were observed to be lying on side or back and abnormally swift swimming with the test concentration no less than 30 mg/L. Fishes treated with 50 mg/L test substance were observed to be tumbling during swimming. Under the study conditions, the 96 h LC50 and NOEC of the test substance were determined to be 29.4 and 20 mg/L (nominal), corresponding to 27.7 and 19.2 mg/L (measured concentrations), respectively (Key Lab., 2010).
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